Features

MG instrumentation (Magnetic Force Generator, MFG)

• Long working distance (~1 meter: similar to MRI bore size)

• Scalable: 16-, 35-, and 70-cm diameter à larger setup is possible

• Stimulation frequency: up to 10 Hz (permanent magnet), ~30 Hz (electromagnet)

Mechanosensitive proteins

• Versatile platform for diverse mechanosensitive ion channels & receptors

• Ion channels: (for excitation) Piezo1/2, TRP family, (for inhibition) FLYC1

• Receptors: T cell receptor, Notch (synNotch), integrin,…

• Delivery: viral (AAV, Lenti, AV), non-viral (LNP)

Target molecules:

Our MG is the versatile platform for any in vivo mechanobiology systems

Mechanosensitive ion channels (Piezo1/2, TRP, FLYC…)

Mechanosensitive receptors (T cell receptor, Notch/synNotch, Integrin…)

Molecular specificity is achieved by nanoparticles selectively binding and activating target mechanoreceptors via antigen-antibody interaction

Cellular specificity is achieved by selective genetic encoding of mechanosensitive ion channels in any target neuronal types

- genetic: neuron-type specific promoter, Cre-loxP, transgenic animal

- non-genetic: tissue-specific virus

Temporal control:

With current rotating magnetic field setup, continuous or pulsed (up to 10 Hz) stimulation is possible.

New electromagnetic setups being developed for >30 Hz stimulation

Fast cellular response:

Direct force delivery and activation of ion channels

Nearly instantaneous activation & deactivation depending on ion channel gating kinetics (<10 ms)