Research Skills

Research Skills

I am trained in the following techniques

Molecular biology Skills

• Mammalian cell culture: Culture of cell lines in 2D and 3D environments.

• Nucleic acid manipulation: Transfection, transduction, RNAi, and establishment of stable cell lines

• Cytolytic assay: practical approaches to study Natural killer (NK) cell and T cell effector functions

• Polymerase Chain Reaction (PCR): Primer design, optimization, nested PCR, Real Time-qPCR

• Electrophoresis: Agarose and polyacrylamide gel electrophoresis for nucleic acids and proteins

• Microscopy: General fluorescence, wide-field, and confocal imaging techniques

• Microbial cell culture: plasmid DNA isolation, purification, antibiotic susceptibility, essential oil extraction, and characterization

Immunology skills

• Flow cytometry, Fluorescent activated cell sorting (FACS) analysis, Antibody panel design

• Animal handling, mouse xenograft models, drug administration, in vivo experimental setup

Analytical techniques

• Use of GC-MS and HPLC to analyze, purify and characterize various compounds

Computational skills

• Bioinformatics databases such as NCBI and cBioPortal for Cancer Genomics

• xCELLigence Real-Time Cell Analyzers (RTCA ACEA Biosciences)

• Statistical analysis using GraphPad Prism and SPSS

• Microsoft Office packages, Endnote, Adobe packages