Publications & CV
Publications & CV
Embedded Files
Publications
Publications
Illuminating Extracellular Vesicles Biology with Super-Resolution Microscopy: Insights into Morphology and Composition L. Alexandre*, D. D’Arrigo*, N. Kuszla, A. Cronemberger Andrade, A. K A Silva, C. Schietroma, F. Gazeau, Q. Lubart, S. Mangenot ACS Nano, 2025
https://doi.org/10.1021/acsnano.5c00380
ArXiv manuscript https://doi.org/10.48550/arXiv.2501.03713
In situ investigation of extracellular vesicles in viscous formulations: interplay of nanoparticle transport and nanorheology through interferometric light microscopy analysis L. Alexandre, A. Dubrova, A. Kunduru, M. Berger, I. Boucenna, F. Gazeau, A. K. A. Silva, S. Mangenot, K. Aubertin Small Science 2024
https://doi.org/10.1002/smsc.202400319
Effect of Sample Preprocessing and Size-Based Extraction Methods on the Physical and Molecular Profiles of Extracellular Vesicles L. Alexandre, M. L Shen, L. Oliveira Fernandes de Araujo, J. Renault, P. DeCorwin-Martin, R. Martel, A. Ng, D. Juncker ACS Sensors (2024) https://pubs.acs.org/doi/10.1021/acssensors.3c02070
BioRxiv manuscript 2023 https://doi.org/10.1101/2023.07.17.549204
Acoustic detection of a mutation-specific Ligase Chain Reaction based on liposome amplification N. Naoumi, M. Araya-Farias, M. Megariti, L. Alexandre, G. Papadakis, S. Descroix and E. Gizeli Analyst (2024) https://doi-org/10.1039/D3AN02142D
Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets S. Dumas, L. Alexandre, M. Richerd; M. Serra; S. Descroix In: Taly, V., Descroix, S., Perez-Toralla, K. (eds) Microfluidics Diagnostics. Methods in Molecular Biology, vol 2804. Humana, New York, NY (2024). https://doi.org/10.1007/978-1-0716-3850-7_10
High-throughput extraction on a dynamic solid phase for low-abundance biomarker isolation from biological samples L. Alexandre, M. Araya-Farias, M.-L. Nguyen, N. Naoumi, G. Gropplero, E. Gizeli, L. Malaquin, S. Descroix Microsystems & Nanoengineering 9, 109 (2023) https://doi.org/10.1038/s41378-023-00582-4
Advances in extracellular vesicle analysis L. Alexandre, J. Sun, M. Taverna, W. Zhong Anal Bioanal Chem 415, 1235–1238 (2023) https://doi.org/10.1007/s00216-023-04536-7
Protein Co-Enrichment Analysis of Extracellular Vesicles M. L. Shen, Z. Jin, R. Martel, A. Wallucks, L. Alexandre, P. DeCorwin-Martin, L. Oliveira Fernandes de Araujo, A.Ng, D. Juncker https://arxiv.org/abs/2301.04051
Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid L. Alexandre, A. Bendali, I. Pereiro, M Azimani, S Dumas, L Malaquin, TD Mai and S Descroix Scientific Reports, 2022, 12 (1), 1-11 https://doi.org/10.1038/s41598-022-13304-z
Development of a microfluidic droplet platform with an antibody-free magnetic-bead-based strategy for high through-put and efficient EVs isolation M Morani, M Taverna, Z Krupova, L Alexandre, P Defrenaix, TD Mai Talanta, 2022, 123625 https://doi.org/10.1016/j.talanta.2022.123625
VEGF functionalized magnetic beads in a microfluidic device to improve the angiogenic balance in preeclampsia E. Lecarpentier, L. Trapiella Alfonso, L. Alexandre, S. Descroix, N. Eilstein, V. Tsatsaris American Journal of Obstetrics & Gynecology, 222, s325, 2020 https://doi.org/10.1016/j.ajog.2019.11.514
VEGF (Vascular Endothelial Growth Factor) Functionalized Magnetic Beads in a Microfluidic Device to Improve the Angiogenic Balance in Preeclampsia L. Trapiella-Alfonso*, L. Alexandre*, C. Fraichard, K. Pons, S. Dumas, L. Huart, J.-F. Gaucher, M. Hebert-Schuster, J. Guibourdenche, T. Fournier, M. Vidal, I. Broutin, L. Lecomte-Raclet, L. Malaquin, S. Descroix, V. Tsatsaris, N. Gagey-Eilstein, E. Lecarpentier Hypertension, 2019, 74:145–153 https://doi.org/10.1161/HYPERTENSIONAHA.118.12380
A microfluidic fluidized bed to capture, amplify and detect bacteria from raw samples L. Alexandre, I. Pereiro, A. Bendali, S. Tabnaoui, J. Srbova, Z. Bilkova, S. Deegan, L. Joshi, J.-L. Viovy, L. Malaquin, B. Dupuy, S. Descroix Methods in cell biology, 2018 https://doi.org/10.1016/bs.mcb.2018.07.001
Microfluidic platform to restore the angiogenic balance in preeclampsia L. Alexandre, L. Trapiella Alfonso, N. Eilstein, J. Guibourdenche, E. Lecarpentier, V. Tsatsaris, J.-L. Viovy, L. Malaquin, S. Descroix Pregnancy Hypertension Volume 13, Supplement 1, October 2018, Page S104 https://doi.org/10.1016/j.preghy.2018.08.308
A new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples I. Pereiro, A. Bendali*, S. Tabnaoui*, L. Alexandre*, J. Srbova, Z. Bilkova, S. Deegan, L. Joshi, J.-L. Viovy, L. Malaquin, B. Dupuy, S. Descroix Chem. Sci., 2017, 8, 1329-1336 https://doi.org/10.1039/C6SC03880H
Publications from international conferences
A novel method of size measurement in super-resolution microscopy for extracellular vesicles characterization N. Kuszla, L. Alexandre, F. Gazeau, S. Mangenot Conference ISEV, 2025
Label-free sizing and multiplexed protein detection on single extracellular vesicles A. Wallucks, P. DeCorwin-Martin,M. L. Shen, L. Alexandre, H. Martin, A. Ng, D. Juncker Conference ISEV, 2025
Multiplexed protein detection on single extracellular vesicles by DNA Exchange Imaging A. Wallucks, P. DeCorwin-Martin, L. Alexandre, J. Renault, R. Martel, M. L. Shen, L. Oliveira Fernandes De Araujo, A. Ng, D. Juncker Conference ISEV, 2022 https://doi.org/10.1002/jev2.12224
On-chip magnetic immuno-extraction of small extracellular vesicles from human plasma M. Araya-Farias, D. Brambilla, L. Alexandre, L. Trapiella-Alfonso, G. Gropplero, M. Verhursel, W. Cesar, M. Chiari, T.-D. Mai, M. Morani, S. Descroix, Conference ISEV, 2021 https://doi.org/10.1002/jev2.12083
Characterization of the extraction of extracellular vesicles using a lab on-a-disc filtration system L. Alexandre, P. DeCorwin-Martin, R. Martel, M. Shen, J. Renault, L. Oliveira, A. Ng, D. Juncker Conference ISEV, virtual, 2020 https://doi.org/10.1080/20013078.2020.1784511
Combinatorial antibody microarray profiling of intra- and extravesicular proteins in colorectal cancer cell line extracellular vesicles R. Martel, M. Shen, P. DeCorwin-Martin, L. Alexandre, L. Oliveira, A. Ng, D. Juncker Conference ISEV, virtual, 2020 https://doi.org/10.1080/20013078.2020.1784511
Normalized extravesicular protein expression profiles on antibody microarrays reveal protein associations in EVs of organotropic and metastatic breast cancer cell lines M. Shen, R. Martel, L. Alexandre, P. DeCorwin-Martin, G. Ongo, A. Ng, L. Oliveira, D. Juncker Conference ISEV, virtual, 2020 https://doi.org/10.1080/20013078.2020.1784511
A microfluidic module for extracellular vesicle separation coupled to microarray-based phenotyping M. Cretich, D. Brambilla, A. Romanato, M. T. Odinolfi, S. Descroix, L. Alexandre, L. Trapiella, M. S. Ünlü, N. Zarovni M. Chiari Conference ISEV, 2019 https://doi.org/10.1080/20013078.2019.1593587
Development of a fully automated microfluidic magnetic fluidized bed for circulating tumor DNA detection L. Alexandre, K Perez, JL Viovy, S Descroix, L Malaquin PrCLINICAL & EXPERIMENTAL METASTASIS, 2018
Microfluidic magnetic fluidized bed for bacteria extraction, growth and detection I Pereiro, J Kucerova, L. Alexandre, B Dupuy, Z Bilkova, JL Viovy, L Malaquin, S Descroix MicroTAS, 2014
Curriculum Vitae
Curriculum Vitae
September 2023
to now
Professionnal experience
Professionnal experience
Senior postdoctoral researcher team of Florence Gazeau, Université Paris Cité
Exploration of mRNA loading strategies for extracellular vesicles engineering
PEPR CARN project
May 2020 to
July 2023
Marie Skłodowska-Curie Action fellowship in between Institut Curie and McGill University under the supervision of David Juncker and Stéphanie Descroix
Microfluidic platform for proteomic and genetic extracellular vesicles detection – extraction from bodily fluids, samples storage, RT qPCR, fluorescent detection
March 2019 to
April 2020
Postdoctoral position in Nano and Micro Engineering in the department of Biomedical Engineering of McGill University under the supervision of David Juncker, McGill University, Montréal, Canada
Development of nanoarrays for single extracellular vesicles (EVs) characterization
October 2018 to
March 2019
Postdoctoral position in MMBM Group, under the supervision of Stephanie Descroix, Institut Curie / Institut Pierre Gilles de Gennes (IPGG), Paris, France
Adaptation of a system of microfluidic magnetic fluidized bed to the animal model (mice) for the ANR Apherese
October 2015 to
October 2018
PhD Candidate in collaboration between Jean-Louis Viovy’s team and ELiA team under the supervision of Stephanie Descroix and Laurent Malaquin, Institut Curie / IPGG, Paris, France and LAAS-CNRS, Toulouse, France
Development of the system of microfluidic magnetic fluidized bed for biomedical applications at the interface physics/biology, skills developed in bioassay, microbiology, magnetic field, imaging, microfabrication and microsystems
October 2015 to
October 2018
Presenter at the Museum ‘Palais de la Découverte’, Paris, France
Scientific mediation in diverse fields of physics (electricity, mechanics, chemistry)
March 2015 to
July 2015
Research trainee in the Complex Fluid Group at the Mechanical and Aerospace Engineering department under the direction of Pr Howard Stone, Princeton, US
Study of biofilms in microfluidics, skills in confocal imaging, PIV and microbiology
September 2014 to
March 2015
Assistant Manager of the Scientific Attaché at the Consulate General of France in Houston, Texas, US Work on scientific exchanges between American and French PhD
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