Lasy-Seq protocol

About this site

This is a site to provide updates of protocol of Lasy-Seq, a high throughput and cost-effective RNA-Seq library preparation method. The original protocols was published in Kamitani et al. (2019) Scientific Reports.

Publication

Protocols

Version 1.1

Protocol with 5x WGS kits (Enzymatics) instead of Nextera transposase.

10/24/2019

Version 1.0

Protocol published as supplementary note 1 of Kamitani et al. 2019.

05/10/2019

Version 0.9

Protocol for SE sequence with RNase treatment and Nextera tagmentation. Optimization of input for Nextera reaction was not conducted.

05/10/2019

Version 0.8

Protocol for PE sequence without RNase treatment.

05/10/2019