ラット生殖工学技術

ラット生殖工学技術（ラット新鮮、冷蔵、凍結精子を用いた体外受精、凍結精子の輸送、超過剰排卵誘発剤の開発、受精卵の凍結保存、凍結受精卵を用いたゲノム編集技術など）がほぼ確立されました。

以下、ご興味があれば、ご一読いただければ幸いです。

1 Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats. 

Nakagata N, Mikoda N, Nakao S, Nakatsukasa E, Takeo T.

Sci Rep. 2020 Jan 9;10(1):93.

doi: 10.1038/s41598-019-57090-7. PMID: 31919412. Free PMC article.

2 Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days. 

Yamaga K, Nakao S, Mikoda N, Yoshimoto H, Nakatsukasa E, Nakagata N, Takeo T.

Sci Rep. 2021 Nov 22;11(1):22641.

doi: 10.1038/s41598-021-02166-6. PMID: 34811440. Free PMC article.

3 Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat. 

Takeo T, Nakao S, Mikoda N, Yamaga K, Maeda R, Tsuchiyama S, Nakatsukasa E, Nakagata N.

Lab Anim (NY). 2022 Oct;51(10):256-274.

doi: 10.1038/s41684-022-01053-5. PMID: 36216983. Review.

4 Observation of the in vitro fertilization process in living oocytes using frozen-thawed sperm in rats. 

Nakagata N, Nakao S, Mikoda N, Yamaga K, Takeo T.

Theriogenology. 2023 Mar 15;199:69-76.

doi: 10.1016/j.theriogenology.2023.01.016. PMID: 36696771. Free article.

5 High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm. 

Yamaga K, Nakao S, Mikoda N, Sztein JM, Nakagata N, Takeo T.

J Reprod Dev. 2024 Apr 4;70(2):131-137.

doi: 10.1262/jrd.2023-085. PMID: 38432992. Free PMC article.

6 Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes. 

Nakagata N, Nakao S, Mikoda N, Yamaga K, Takeo T.

J Reprod Dev. 2024 Apr 4;70(2):123-130.

doi: 10.1262/jrd.2023-067. PMID: 38403585. Free PMC article.

7 Birth of offspring derived from cryopreserved rat sperm after shipment in a Styrofoam box at -80°C.

Nakagata N, Nakao S, Mikoda N, Yamaga K, Suzuki H, Takeo T.

Exp Anim. 2025 Jun 26.

doi: 10.1538/expanim.25-0041. PMID: 40571596. Free article.

8 Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age.

Nakagata N, Nakao S, Mikoda N, Yamaga K, Nakagawa Y, Sakuma T, Yamamoto T, Takeo T.

PLoS One. 2025 Sep 8;20(9):e0328718.

doi: 10.1371/journal.pone.0328718. PMID: 40920796. Free PMC article.

なお、詳細は、以下のオンラインショートセミナーでご説明いたします。

11月28日（金）12:15~：第7回　ラット生殖工学技術開発への挑戦　『前編：20年間は、失敗の繰り返し！』

12月26日（金）12:15~：第8回　ラット生殖工学技術開発への挑戦　『後編：ラット生殖工学技術の確立』