PUBLICACIONES

1. Gomez-Lugo, J.J.; Casillas-Vega, N.G.; Gomez-Loredo, A.; Balderas-Renteria, I.; Zarate, X. High-Yield Expression and Purification of Scygonadin, an Antimicrobial Peptide, Using the Small Metal-Binding Protein SmbP. Microorganisms 2024, 12, 278. https://doi.org/10.3390/microorganisms12020278 

2. Narh, J.K.; Casillas-Vega, N.G.; Zarate, X. LL‐37_Renalexin hybrid peptide exhibits antimicrobial activity at lower MICs than its counterpart single peptides. Appl. Microbiol. Biotechnol. 2024, 108, 126. doi.org/10.1007/s00253-023-12887-5 

3. Gonzalez-Contreras, F.; Zarate, X. Neutrophil extracellular traps: Modulation mechanisms by pathogens. Cell. Immunol. 2022, 382, 104640. https://doi.org/10.1016/j.cellimm.2022.104640

4. Martínez-Mora, E.; Arredondo-Espinoza, E.; Casillas-Vega, N.G.; Cantú-Cárdenas, M.E.; Balderas-Rentería, I.; Zarate, X. The Small Metal-Binding Protein SmbP Improves the Expression and Purification of the Recombinant Antitumor-Analgesic Peptide from the Chinese Scorpion Buthus martensii Karsch in Escherichia coli. Curr. Issues Mol. Biol. 2022, 44, 550-558. https://doi.org/10.3390/cimb44020038 

5. Perez-Perez, D.A.; Villanueva-Ramirez, T.d.J.; Hernandez-Pedraza, A.E.; Casillas-Vega, N.G.; Gonzalez-Barranco, P.; Zarate, X. The small metal-binding protein SmbP simplifies the recombinant expression and purification of the antimicrobial peptide LL-37. Antibiotics, 2021, 10, 1271. doi.org/10.3390/antibiotics10101271 

6. Ochoa-Diaz, D.; Mendoza-Olazaran, S.; Zarate, X.; Casillas-Vega, N. Meta-analysis of diagnostic tests for the detection of COVID-19. Rev. Med. Inst. Mex. Seguro Soc. 2021, 59, 182-188. revistamedica.imss.gob.mx/editorial/index.php/revista_medica/article/view/4016 

7. Gomez-Lugo, J.J.; Santos, B.D.; Perez-Perez, D.A.; Montfort-Gardeazabal, J.M.; McEvoy, M.M.; Zarate, X. Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding proteins SmbP and CusF3H+. In: Protein downstream processing: design, development and application of high and low-resolution methods, second edition. Edited by: Labrou N. Methods in Molecular Biology, vol. 2178, Humana Press, New York, 2021, pp 329-344. doi.org/10.1007/978-1-0716-0775-6_22 

8. Martinez-Mora, E.; Gonzalez-Gonzalez, M.d.R.; Zarate, X.; Carranza-Rosales, P.; Ramirez-Cabrera, M.A.; Balderas-Renteria, I.; Arredondo-Espinoza, E. Enhanced in vitro anticancer activity of yeast expressed recombinant glucose oxidase versus commercial enzyme. Appl. Microbiol. Biotechnol. 2021, 105, 2377-2384. doi.org/10.1007/s00253-021-11179-0 

9. Montfort-Gardeazabal, J.M.; Morales-San Claudio, P.C.; Casillas-Vega, N.G.; Zarate, X. Expression and Purification of the VpDef Defensin in Escherichia coli Using the Small Metal-Binding Proteins CusF3H+ and SmbP. Protein Peptide Lett. 2021, 28, 108-114. dx.doi.org/10.2174/0929866527666200610133407 

10. Montfort-Gardeazabal, J.M.; Balderas-Renteria, I: Casillas-Vega, N.G.; Zarate, X. Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP. Protein Expres. Purif. 2021, 178, 105784. doi.org/10.1016/j.pep.2020.105784 

11. Vázquez-Rodríguez, A.; Vasto-Anzaldo, X.G.; León-Buitimea, A.; Zarate, X.; Morones-Ramírez, J.R. Antibacterial and antibiofilm activity of synthesized silver nanoparticles coated with exopolysaccharides obtained from Rhodotorula mucilaginosa. IEEE T. Nanobiosci. 2020, 19, 498-503. doi.org/10.1109/TNB.2020.2985101 

12. Perez-Perez, D.A.; Pioquinto-Avila, E.; Arredondo-Espinoza, E.U.; Morones-Ramirez, J.R.; Balderas-Renteria, I.; Zarate, X. Engineered small metal-binding protein tag improves the expression and purification of recombinant human growth hormone in the periplasm of Escherichia coli. FEBS Open Bio 2020, 10, 546-551. doi.org/10.1002/2211-5463.12808 

13. Casillas Vega, N; Zarate Kalfopulos, X. Técnicas de Microbiología Molecular. In: Procedimientos de Microbiología Médica Diagnóstica, first edition. Edited by: Casillas Vega, N., Mendoza Olazarán, S. Flores Aréchiga, A. McGraw-Hill Interamericana editores, Ciudad de México 2020, pp 139-148.

14. Santos, B.D.; Morones-Ramirez, J.R.; Balderas-Renteria, I.; Casillas-Vega, N.G.; Galbraith, D.W.; Zarate, X. Optimizing periplasmic expression in Escherichia coli for the production of recombinant proteins tagged with the small metal-binding protein SmbP. Mol. Biotechnol. 2019, 61, 451-460. doi.org/10.1007/s12033-019-00176-4 

15. Vázquez-Rodríguez, A.; Vasto-Anzaldo, X.G.; Barboza-Pérez, D.; Vázquez-Garza, E.; Chapoy-Villanueva, H.; García-Rivas, G.; Garza-Cervantes, J.A.; Gómez-Lugo, J.J.; Gómez-Loredo, A.E.; Garza-González, M.T.; Zárate, X.; Morones-Ramírez, J.R. Microbial competition of Rhodotorula mucilaginosa UANL-001L and E. coli increase biosynthesis of non-toxic exopolysaccharide with applications as a wide-spectrum antimicrobial. Sci. Rep. 2018, 8,798. doi.org/10.1038/s41598-017-17908-8 

16. Garza-Cervantes, J.A.; Chávez-Reyes, A.; Castillo, E.C.; García-Rivas G.; Ortega-Rivera, O.A.; Salinas, E.; Ortíz-Martínez, M.; Gómez-Flores S.L.; Peña-Martínez, J.A.; Pepi-Molina, A.; Treviño-González, M.T.; Zarate, X.; Cantú-Cárdenas, M.E.; Escárcega-González, C.E.; Morones-Ramírez, J.R. Synergistic antimicrobial effects of silver/transition-metal combinatorial treatments. Sci. Rep. 2017, 7, 903. doi.org/10.1038/s41598-017-01017-7 

17. Vargas-Cortez, T.; Morones-Ramírez, J.R.; Balderas-Rentería, I.; Zarate, X. Production of recombinant proteins in Escherichia coli tagged with the fusion protein CusF3H+. Protein Expres. Purif. 2017, 132, 44-49. dx.doi.org/10.1016/j.pep.2017.01.006 

18. Cantú-Bustos, J.E.; Cano del Villar, K.D.; Vargas-Cortez, T.; Morones-Ramírez, J.R.; Balderas-Rentería, I.; Zarate, X. Recombinant protein production data after expression in the bacterium Escherichia coli. Data Brief 2016, 7, 502-508. dx.doi.org/10.1016/j.dib.2016.02.074 

19. Garza Gonzalez, M.T.; Barboza Pérez, D.; Vázquez Rodríguez, A.; García-Gutiérrez D.I.; Zarate, X.; Cantú-Cárdenas, M.E.; Urraca-Botello, L.I.; López-Chucken, U.J.; Treviño-Torres, A.L.; Cerino-Córdoba, F.J.; Medina-Ruiz, P.; Villarreal-Chiu, J.F.; Morones-Ramírez, J.R. Metal-induced production of a novel bioadsorbent exopolysaccharide in a native Rhodotorula mucilaginosa from the Mexican northeastern region. PLoS One 2016, 11, e0148430. doi.org/10.1371/journal.pone.0148430 

20. Cantú-Bustos, J.E.; Vargas-Cortez, T.; Morones-Ramírez, J.R.; Balderas-Rentería, I.; Galbraith, D.W.; McEvoy, M.M.; Zarate, X. Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF. Protein Expres. Purif. 2016, 121, 61-65. dx.doi.org/10.1016/j.pep.2016.01.007 

21. Vargas-Cortez, T.; Morones-Ramírez, J.R.; Balderas-Rentería, I.; Zarate, X. Expression and purification of recombinant proteins in Escherichia coli tagged with a small-metal binding protein from Nitrosomonas europaea. Protein Expres. Purif. 2016, 118, 49-54. dx.doi.org/10.1016/j.pep.2015.10.009 

22. Zarate, X.; McEvoy, M.M.; Vargas-Cortez, T.; Gómez-Lugo, J.J.; Barahona, C.J.; Cantú-Cárdenas, M.E.; Gómez-Treviño, J.A. Purification of the Drosophila melanogaster proteins Inscuteable and Staufen expressed in Escherichia coli. Advances Bioscience Biotechnol. 2015, 6, 485-493. dx.doi.org/10.4236/abb.2015.67050 

23. Zarate, X.; Galbraith, D.W. A cell-free expression platform for production of protein microarrays. In: Cell-Free Protein Synthesis: Methods and Protocols. Edited by: Alexandrov K. y Johnston W.A. Methods in Molecular Biology, vol. 1118, Humana Press, Nueva York 2014, pp 297-307. link.springer.com/protocol/10.1007/978-1-62703-782-2_21 

PATENTES

Zarate Kalfopulos, X.; Vargas Cortez, T.; Cantú Bustos, J.E. Sistema de expresión y purificación de proteínas recombinantes marcadas con CusF3His+ como proteína de fusión. Solicitud de patente ante el Instituto Mexicano de la Propiedad Industrial: MX/a/2015/014635. Título: 378537, otorgado diciembre 2020.

Zarate Kalfopulos, X.; Vargas Cortez, T. Sistema de expresión y purificación de proteínas recombinantes marcadas con SmbP como proteína de fusión. Solicitud de patente ante el Instituto Mexicano de la Propiedad Industrial: MX/a/2015/010453. Título: 370879, otorgado diciembre 2019.