本實驗室致力於食品微生物、食品安全與快速檢測技術之研究，主要探討食品在抗生素抗藥性菌株傳播中的潛在角色，並結合生物感測與生物晶片技術，發展快速且具應用潛力的食源性病原菌檢測方法。研究方向主要分為以下兩大主軸：
抗生素抗藥性（Antimicrobial Resistance, AMR）已成為全球重要的公共衛生議題。除了醫療環境外，食品亦可能成為抗藥性菌株及其抗藥性基因在人類、環境與食物鏈之間傳播的重要媒介。
本實驗室著重於食品中抗生素抗藥性菌株的分離、鑑定與抗藥性特徵分析，透過分析食品來源菌株的抗藥性表型與抗藥性基因，希望進一步了解食品在抗藥性菌傳播中的潛在角色，並建立食品安全與抗生素抗藥性監測的科學基礎。
傳統培養與菌落形成單位（Colony-Forming Unit, CFU）計數是食品微生物檢測的重要方法，但通常需要經過樣品稀釋、培養及菌落計數等步驟，且至少需要約 24 小時才能取得結果。因此，如何縮短檢測時間並快速評估食品中的活菌數量，是食品安全檢測的重要研究方向。
本實驗室致力於將生物晶片（Biochip）應用於食源性病原菌檢測，透過場效電晶體生物感測器（Biosensor Field-Effect Transistor, Bio-FET）之開發與應用，期望透過生物感測技術建立快速、靈敏且具實際應用潛力的微生物檢測平台，以縮短傳統培養方法所需時間，並進一步應用於食品安全監測。
Our laboratory focuses on food microbiology, food safety, and rapid microbial detection technologies. Our research aims to understand the potential role of food in the dissemination of antibiotic-resistant bacteria and to develop rapid detection methods for foodborne pathogens through biosensing and biochip technologies. Our research encompasses two major areas:
Antimicrobial resistance (AMR) has become a major global public health concern. In addition to healthcare settings, food may serve as an important vehicle for the transmission of antibiotic-resistant bacteria and resistance genes among humans, the environment, and the food chain.
Our laboratory focuses on the isolation, identification, and characterization of antibiotic-resistant bacteria from food. By investigating antimicrobial resistance phenotypes and resistance-associated genes in foodborne isolates, we aim to better understand the potential role of food in AMR dissemination and provide scientific evidence for food safety and antimicrobial resistance surveillance.
Conventional culture-based methods and colony-forming unit (CFU) counting remain important approaches for microbial assessment in food. However, these methods involve multiple labor-intensive procedures, including sample dilution, plating, incubation, and colony counting, and generally require at least 24 hours before results can be obtained. Therefore, developing rapid approaches for the detection and quantification of viable bacteria is an important goal in food safety testing.
Our laboratory focuses on the application of biochip for the rapid detection of foodborne pathogens, with particular interest in the development and application of biosensor field-effect transistor (Bio-FET) platforms. Our goal is to establish rapid, sensitive, and practical microbial detection systems that reduce the time required by conventional culture-based methods and facilitate their future application in food safety monitoring.
歡迎對微生物及生物晶片有興趣的學生與PI聯絡！
Email: KuoCH.Mark@outlook.com; 108927@ctust.edu.tw