Synergistical effect of various organelles (organelle interactions) is increasingly recognized to have a vital role in executing diverse physiological cellular functions. Fluorescence microscopy, with target specificity and compatibility with live cells, is a valuable tool for studying complex biological systems and processes. We aim to develop three-dimensional, highly multiplexed, and high-speed spectral microscopy techniques to visualize intracellular activities and quantify chemical/physical parameters of cellular organelles at high spatiotemporal resolution.

As a bottom-up approach, super-resolution microscopy through single-molecule localization achieves ~10 nm spatial resolution that challenges the mindset of traditional microscopy. By characterizing the multidimensional information of single-molecule signals, multifunctional super-resolution microscopy are developed for cell biology beyond the structural information.

Three-dimensional dual-comb CARS imaging of the mixture of RA and β-carotene.

Coherent Raman microscopy provides label-free imaging by interrogating the intrinsic vibration of molecules. Spectral focusing dual-comb CARS might now have advanced potential to evolve into a tool for label-free chemical imaging of highly dynamic and short-lived transient objects in chemical and biological system.