Templated chemistry has been achieved due to the enhanced local concentration of reactants arising from attractive interactions between biomolecules. Through this unique reaction, several biomolecular operations, such as synthesis, cleavage, and transformation, can be conducted. Based on these operations, we develop biomolecular circuits that can be employed for important diagnostic and therapeutic applications, including biomolecule sensing, site-specific functionalization of biomolecules, bioactive molecule (drug) release, and the regulation of biological processes.

DNA-encoded libraries (DELs) have become an important platform in modern drug discovery by enabling the efficient screening of vast small-molecule chemical space. DEL construction relies on on-DNA synthesis, where diverse compounds are assembled under DNA-compatible conditions while preserving the encoding tags. In this context, we pursue research to develop and expand robust on-DNA reactions and scaffold-forming strategies, ultimately advancing DEL-based hit discovery and lead generation.