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Research Interest
Standardization of organoid models
Standardization of Extracellular vesicles (EVs) (ex, Exosomes)
Quantification of viral genome (ex, SARS-CoV-2)
Genome engineering using CRISPR system
Liquid biopsy for diagnosis using Circulating Tumor Nucleic Acids (ctDNA, cfDNA, miRNA etc)
Cancer genomics using Next Generation Sequencing (NGS)
Post-translational modification in cancer (Ubiquitination, Ufmylation, Sumoylation, ISGylation)
Establishment of national measurement standards
Development of Certified Reference Materials (CRM)
Proteomics and metabolomics using mass spectrometry
Standardization in organoid research is vital for overcoming these challenges. A standardized framework for the culture of organoids, including protocols for cell isolation, media composition, passaging, and quality control, will enable researchers to generate reproducible results. This uniformity will encourage cross-laboratory cooperation and the exchange of information and resources, enhancing our knowledge of the gut and hastening the development of cures.
We tried to establish the best protocol guidelines, quality control measures, and reference materials development. Standardization of organoid culture is paramount for realizing the full potential of this usual tool in gastroenterology and regenerative medicine.
The international scientific community can coordinate efforts, resulting in more reliable research findings, better medication development, and better clinical outcomes by adopting standard protocols and exchanging expertise.
Lee JE, Jeong SY, Li Z, Kim HY, Kim HW, Yoo MJ, Jang HJ, Kim DK, Cho N, Yoo HM*, Kim KH*. Development of a screening platform to discover natural products active against SARS-CoV-2 infection using lung organoid models. Biomaterials Research. 2023 Mar 1;27(1):18. [ IF= 15.863]
UFM1, ubiquitin-fold modifier 1, is a small protein (9 kDa) that is the most recently identified ubiquitin-like protein. Protein modification by UFM1 (ufmylation) follows a mechanism closely related to ubiquitin system: UFM1 is activated by the E1 enzyme UBA5, conjugated by the E2 enzyme UFC1, and ligated to its substrates by E3 ligase UFL1. Ufmylation is a reversible process that is catalyzed by UFM1-specific proteases (UFSPs) and only one functional UFSP2 is expressed in human. It may be possible that UFM1 system acts as a key factor that is essential for proliferation of a large subset of tumors.
Yoo HM, Kang SH, Kim JY, Lee JE, Seong MW, Lee SW, Ka SH, Sou YS, Komatsu M, Tanaka K, Lee ST, Noh DY, Baek SH, Jeon YJ, Chung CH. Mol Cell. 2014 Oct 23;56(2):261-274.
Yoo HM, Park JH, Jeon YJ, Chung CH (2015) Ubiquitin-fold modifier 1 acts as a positive regulator of breast cancer. Front Endocrinol. 2015 Mar 20;6:36.
Fang B, Li Z, Qiu Y, Cho N, Yoo HM. Biomolecules. 2021 Sep 11;11(9):1348.
Yoo HM, Park JH, Kim JY, Chung CH. Mol Cells. 2022 Jun 30;45(6):425-434.
Chung CH, Yoo HM. Biochem Biophys Res Commun. 2022 Dec 10;633:61-63.
The Center for Bio-analysis is conducting researches on establishing ‘measurement standards for major bio-materials’ such as DNA, proteins, sugars and metabolites. We are also conducting researches to expand the established ‘measurement standards on bio-materials’ to ‘measurement standards on living things’ such as cells, microorganisms, and viruses and ‘measurement of complex life phenomena’ such as diseases, immunity, and biological reactions. Establishment of bio-measurement standards includes the development of primary measurement methods, ensuring international measurement equivalence, and the development and dissemination of reference materials on biological measurands.
Established bio-measurement standards are used to improve the accuracy and reliability of various bio-analyses such as clinical tests, molecular diagnostics, drug quality control, and food safety tests, thereby contributing to the improvement of the quality of life of the people.
Its current R&D project include the following subjects :
Genetic and genomic measurement standards: Analysis of DNA and RNA, mutation
Protein and protein measurement standards: Proteomics analysis
Clinical Chemistry Measurement Standards: Biomarkers
Cell and microbial measurement standards
• 유전자 및 유전체 측정표준: DNA 및 RNA 정량분석, 돌연변이, 유전체 표준물질
• 단백질 및 단백체 측정표준: 단백질 정량분석, 단백질 표준물질, 단백체 분석
• 임상화학 측정표준: 기초 진단검사, 바이오마커
• 세포 및 미생물 측정표준: 세포 성장 및 사멸 측정, CBC 표준물질, 미생물 정량분석
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