Papers, Posters, & Awards
Grants & Awards:
2018 LIU Pharmacy Internal Research Support Grant (GSTP)
2017 AACP New Investigator Award (Dr. Frey): Pre-Clinical Evaluation of Preclinical Anti-HIV Agents for Mutational Resistance
2017 LIU Pharmacy Internal Research Support Grant (RT)
Selected Abstracts & Presentations:
Risbud, K. and Frey, K.M. (2018) Predicting the Effects of GST Pi Polymorphism on Resistance to Ezatiostat. Experimental Biology, ASPET. San Diego, CA, April 21 – 25, 2018. https://www.fasebj.org/doi/10.1096/fasebj.2018.32.1_supplement.lb97
Muwonge, A., Azeem, S., and Frey, K.M. (2018) Predicting Resistance To Investigational Microbicide MIV-150 Using Structure-based methods and Fluorescence Enzyme Inhibition. Experimental Biology, ASPET. San Diego, CA, April 21 – 25, 2018. https://www.fasebj.org/doi/10.1096/fasebj.2018.32.1_supplement.830.1
Azeem, S., Muwonge, A., Ivatorov, M., Yunayev, M. and Frey, K.M. (2018) Exploiting the Components Leading to Mutational Resistance in Rigid and Flexible Non-nucleoside Reverse Transcriptase Inhibitors (NNRTIs). Experimental Biology, ASPET. San Diego, CA, April 21 – 25, 2018. https://www.fasebj.org/doi/10.1096/fasebj.2018.32.1_supplement.828.4
Alghamdi, A. and Frey, K.M. (2017) Predicting the Toxic Effect of Organophosphates on GST Enzyme Isoforms. Experimental Biology/ASPET. Chicago. IL, USA. http://www.fasebj.org/content/31/1_Supplement/lb623.abstract?sid=ae5bf61c-18c7-4c18-a7dd-40a6a9c382ba
Narke, D., Kurade, M., Dholakia, K., Siddique, A., Thakkar, N., Frey, K.M., and Ponnoth, D. (2017) Limonene Reduces Airway Hyperreactivity and Inflammation through Activation of A2A Adenosine Receptors. Experimental Biology/ASPET. Chicago. IL, USA. http://www.fasebj.org/content/31/1_Supplement/820.1.short
Frey, K.M., Azeem, S.M., Shahed, I., Anderson, K.S. (2016) Predicting Resistance Mutations in HIV Reverse Transcriptase. Drug Discovery & Therapy World Congress. Boston, MA, USA. https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=33&ved=0ahUKEwju3s6Sx_nTAhUFJiYKHXUyB6A4HhAWCC4wAg&url=http%3A%2F%2Fglobalbiotechcongress.com%2Fpdf%2FAbstract-Book(2016).pdf&usg=AFQjCNEDxfj5SvR23BTqwFKqYGQjuAxBsw&sig2=mmox7yi42Tt6qHNB5T832w&cad=rja
Frey, K.M., Gray, W.T., Spasov, K.A., Bollini, M., Cisneros, J.A., Gallardo-Masias, R., Jorgensen, W.L., Anderson, K.S. (2013) Structure-based Evaluation of Modified Derivatives in the Catechol Diether Series Targeting HIV-1 Reverse Transcriptase. Cambridge Health Institute: Structure-based Drug Design. Boston, MA, USA.
Frey, K.M., Lombardo, M.N., Wright, D.L., Anderson, A.C. (2010) Towards the Understanding of Mutational Resistance Mechanisms in MRSA DHFR. Keystone Symposia: New Directions in Small Molecule Drug Discovery. Whistler, BC, Canada.
Frey, K.M., Georgiev, I., Donald, B.R., Anderson, A.C. (2010) Predicting Resistance Mutations Using Protein Design Algorithms. Institute for Drug Resistance: Shifting the Paradigms of Drug Resistance. Shrewsbury, MA, USA.
Peer Reviewed Articles:
Azeem, S.M., Muwonge, A.N., Thakkar, N., Lam, K.W., and Frey, K.M. (2018) Structure-based methods to predict mutational resistance to diarylpyrimidine non-nucleoside reverse transcriptase inhibitors. J Mol Graph Model, 79: 133-139. https://doi.org/10.101/j.jmgm.2017.10.023
Frey, K.M. (2015) Structure-enhanced methods in the development of non-nucleoside inhibitors targeting HIV reverse transcriptase variants. Future Microbiol, 10: 1767-1772. http://www.ncbi.nlm.nih.gov/pubmed/26517310
Gray, W.T., Frey, K.M., Laskey, S.B., Mislak, A.C., Spasov, K.A., Lee, W.G., Bollini, M., Siliciano, R.F., Jorgensen, W.L., and Anderson, K.S. (2015) Non-nucleoside Inhibitors Active Against HIV Reverse Transcriptase with K101P, a Mutation that Confers High Level Rilpivirine Resistance. ACS Med Chem Lett, 6: 1075-1079. http://www.ncbi.nlm.nih.gov/pubmed/26487915
Lee. W., Frey, K.M., Gallardo-Macias, R., Spasov, K.A., Chan, A.H., Anderson, K.S., and Jorgensen, W.L. (2015) Discovery and crystallography of bicyclic arylaminoazines as potent inhibitors of HIV-1 reverse transcriptase. Biorg Med Chem Lett, 25: 4824-file://localhost/4827. http/::www.ncbi.nlm.nih.gov:pubmed:26166629
Sohl, C.D., Ryan, M., Luo, B., Frey, K.M., and Anderson, K.S. (2015) Illuminating the molecular mechanism of tyrosine kinase inhibitor resistance for the FGFR1 gatekeeper mutation: the Achilles’ heel of targeted therapy. ACS Chem Biol, 10: 1319-1329. http://www.ncbi.nlm.nih.gov/pubmed/25686244
Frey, K.M., Puleo, D.E., Spasov, K.A., Bollini, M., Jorgensen, W.L. and Anderson, K.S. (2015) Structure-based Evaluation of Non-nucleoside Inhibitors with Improved Potency and Solubility that Target HIV Reverse Transcriptase Variants. J Med Chem, 58: 2737-2745. http://www.ncbi.nlm.nih.gov/pubmed/25700160
Reeve, S.M., Gainza, P., Frey, K.M., Georgiev, I., Donald, B.R., and Anderson, A.C. (2015). Protein Design Algorithms Predict Viable Resistance to an Experimental Antifolate. Proc Natl Acad Sci USA, 112: 749-754. http://www.ncbi.nlm.nih.gov/pubmed/25552560
Lee, W., Frey, K.M., Gallardo-Macias, R., Spasov, K.A., Bollini, M., Anderson, K.S., and Jorgensen, W.L. (2014) Picomolar Inhibitors of HIV-1 Reverse Transcriptase: Design and Crystallography of Naphthyl Phenyl Ethers. ACS Med Chem Lett, 5: 1259-1262. http://www.ncbi.nlm.nih.gov/pubmed/25408842
Kumar, V.P., Cisneros, J.A., Frey, K.M., Castellanos-Gonzalez, A., Wang, Y., Gangjee, A., White Jr., A.C., Jorgensen, W.L., and Anderson, K.S. (2014) Structural studies provide clues for analog design of specific inhibitors of Cryptosporidium hominis Thymidylate Synthase-Dihydrofolate Reductase. Biorg Med Chem Lett, 4: 4158-4161. http://www.ncbi.nlm.nih.gov/pubmed/25127103
Mislak, A.C., Frey, K.M., Bollini, M., Jorgensen, W.L., and Anderson, K.S., (2014). A Mechanistic and Structural Investigation of Modified Derivatives of the Diaryltriazine Class of NNRTIs Targeting HIV-1 Reverse Trancriptase. Biochim Biophys Acta, 1840: 2203-2211. http://www.ncbi.nlm.nih.gov/pubmed/24726448
Frey, K.M., Gray, W.T., Spasov, K.A., Bollini, M., Gallardo-Macias, R., Jorgensen, W.L., and Anderson, K.S. (2014) Structure-based Evaluation of C5 Derivatives in the Catechol Diether Series Targeting HIV-1 Reverse Transcriptase. Chem Biol Drug Des, 83: 541-549 http://www.ncbi.nlm.nih.gov/pubmed/24289305.
Lee, W., Gallardo-Macias, R., Frey, K.M., Spasov, K.A., Bollini, M., Anderson, K.S., and Jorgensen, W.L. (2013) Picomolar Inhibitors of HIV Reverse Transcriptase Featuring Bicyclic Replacement of a Cyanovinylphenyl Group. J Am Chem Soc, 135: 16705-16713. http://www.ncbi.nlm.nih.gov/pubmed/24151856
Iyidogan, P., Sullivan, T.J., Chordia, M., Frey, K.M., and Anderson, K.S. (2013) Design, Synthesis, and Antiviral Evaluation of Chimeric Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase. ACS Med Chem Lett, 4: 1183-1188. http://www.ncbi.nlm.nih.gov/pubmed/24900627
Kumar, V.P., Frey, K.M., Wang, Y., Jain, H.K., Gangjee, A., and Anderson, K.S. (2013) Substituted Pyrrolo[2,3-d]pyrimidines as Cryptosporidium hominis Thymidylate Synthase Inhibitors. Biorg Med Chem Lett, 23: 5426-5428. http://www.ncbi.nlm.nih.gov/pubmed/23927969
Bollini, M., Frey, K.M., Cisneros, J.A., Spasov, K.A., Das, K., Bauman, J.D., Arnold, E., Anderson, K.S., and Jorgensen, W.L. (2013) Extension into the Entrance Channel of HIV-1 Reverse Transcriptase – Crystallography and Enhanced Solubility. Biorg Med Chem Lett, 23: 5209-5212. http://www.ncbi.nlm.nih.gov/pubmed/23899617
Frey, K.M., Bollini, M., Mislak, A.C., Cisneros, J.A., Gallardo-Macias, R., Jorgensen, W.L., and Anderson, K.S. (2012) Crystal Structures of HIV-1 Reverse Transcriptase Reveal Key Interactions for Drug Design. J Am Chem Soc, 134: 19501-19503. http://www.ncbi.nlm.nih.gov/pubmed/23163887
Kim, J., Wang, L., Li, Y., Becnel, K.D., Frey, K.M., Garforth, S.J., Prasad, V.R., Schinazi, R.F., Liotta, D.C., and Anderson, K.S. (2012) Pre-steady State Kinetic Analysis of Cyclobutyl Derivatives of 2'-deoxyadenosine 5'-triphosphate as Inhibitors of HIV-1 Reverse Transcriptase. Biorg Med Chem Lett, 22: 4064-4067. http://www.ncbi.nlm.nih.gov/pubmed/22595174
Frey, K.M., Viswanathan, K., Wright, D.L., and Anderson, A.C. (2012) Prospective Screening of Novel Antibacterial Inhibitors of Dihydrofolate Reductase for Mutational Resistance. Antimicrob Agents Chemother, 56: 3556-3562. http://www.ncbi.nlm.nih.gov/pubmed/22491688
Viswanathan, K., Frey, K.M., Scocchera, E.W., Martin, B.D., Swain III, P.W. Alverson, J., Priestley, N.D., Anderson, A.C., and Wright, D.L. (2012) Toward New Therapeutics for Skin and Soft Tissue Infections: Propargyl-linked Antifolates are Potent Inhibitors of Streptococcus pyognes. PLoS One, 7(2): e29434. http://www.ncbi.nlm.nih.gov/pubmed/22347365
Frey, K.M., Georgiev, I., Donald, B.R., and Anderson, A.C. (2010) Predicting Resistance Mutations Using Protein Design Algorithms. Proc Natl Acad Sci USA, 107: 13707-13712. http://www.ncbi.nlm.nih.gov/pubmed/20643959
Frey, K.M., Lombardo, M.N., Wright, D.L., and Anderson, A.C. (2010) Towards the Understanding of Resistance Mechanisms in Clinically Isolated Trimethoprim-resistant, Methicillin-resistant Staphylococcus aureus Dihydrofolate Reductase. J Struct Biol, 170: 93-97. http://www.ncbi.nlm.nih.gov/pubmed/20026215
Beierlein, J.M.,* Deshmukh, L.,* Frey, K.M.,* Vinogradova, O., and Anderson, A.C. (2009) The Solution Structure of Bacillus anthracis Dihydrofolate Reductase Yields Insight into the Analysis of Structure Activity Relationships for Novel Inhibitors. Biochemistry, 48: 4100-4108. http://www.ncbi.nlm.nih.gov/pubmed/19323450
Frey, K.M., Liu, J., Lombardo, M.N., Bolstad, D.B., Smith, A.E., Priestley, N.D., Wright, D.L., and Anderson, A.C. (2009) Crystallographic Complexes of Wild-type and Mutant MRSA DHFR Reveal an Alternate Conformation of NADPH that May be Linked to Trimethoprim Resistance. J Mol Bio, 387: 1298-1308. http://www.ncbi.nlm.nih.gov/pubmed/19249312
Beierlein, J.M., Frey, K.M., Bolstad, D.B., Pelphrey, P.P., Joska, T., Smith, A.E., Priestley, N.D., Wright, D.L., and Anderson, A.C. (2008) Synthetic and Crystallographic Studies of a New Inhibitor Series Targeting Bacillus anthracis Dihydrofolate Reductase. J Med Chem, 54: 7532-7540. http://www.ncbi.nlm.nih.gov/pubmed/19007108
Bolstad, D.B., Bolstad, E.S., Frey, K.M., Wright, D.L., and Anderson, A.C. (2008) A structure-based approach to the development of potent and selective inhibitors of dihydrofolate reductase from Cryptosporidium. J Med Chem, 51: 6839-6852. http://www.ncbi.nlm.nih.gov/pubmed/18834108