Determining Potential Regulators of SLC30A10 using ENCODE SCREEN Data

The SCREEN function of ENCODE project can be used to search for Candidate Cis-Regulatory Elements (cCRE) of a gene. SCREEN search for human SLC30A10 yields 67 cCREs within the region of SLC30A10. However, most of these cCRE's are very likely not affecting SLC30A10 expression, because they are found at the exonic regions of SLC30A10, have very weak scores for DNAse hypersensitivity and methylation/acetylation, or clearly located close to the transcription start site of other genes, such as lncRNAs.

Limiting the SCREEN of human SLC30A10 to relevant cell types, such as primary hepatocyte samples and HepG2 human liver cancer cell line yields a short list of 4 cCRE's:

ENCODE SCREEN also provides a list of Transcription Factor peaks that intersect with the selected cCRE's. While there are several cell types that show potential binding to the aforementioned 4 cCRE's, limiting the search again to relevant cell lines (hepatocytes, HepG2) yields a list of potential transcription factors: