Education

Career

Career

Ph.D. Scholar

Structural Virology Laboratory

Monash Biomedicine Discovery Institute (BDI), Monash University, Clayton, Australia

Project:

Structural characterization of assembly in Poxviruses

Senior Research Fellow

Structural and Molecular Virology Group

Indian Institute of Technology (IIT) Delhi, India

Project 1:

CryoEM structural studies of proteins in Plasmodium falciparum merozoite surface

Project 2:

Structural characterization of the membrane proteins in Chikungunya virus

Junior Research Fellow

Integrative Structural Biology Lab

National Institute of Immunology (NII), Delhi, India

Project 1:

Structural basis for host-pathogen interaction mediated by DENV EDIII domain

Project 2:

Molecular basis for the function of protein tyrosine phosphatase SHP2

MSc. Biotechnology & Bioinformatics

Institute of Bioinformatics and Applied Biotechnology (IBAB), Bengaluru, Karnataka, India

Areas of study:

Wet Lab:

• Genetic Engineering • Cell & Molecular Biology

• Biochemistry • Immunology • Fermentation

• Structural Biology • Downstream Processing

Dry Lab:

• Omics • Next Gen Sequencing

• Big Data Analysis • Cheminformatics

• C, C++, Python, JAVA, R, and Shell scripting

• Web development & SQL

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BSc. Biotechnology (Honors)

Garden City University, Bengaluru, Karnataka, India

Areas of study:

• Biotechnology • Bioinformatics • Immunology

• Microbiology • Biochemistry • Phytochemistry

• Molecular Biology • Cell Biology • Genetics

• r-DNA Technology • Bioprocess Technology

• Research Methodologies • Intellectual Property Rights

Technical Experience

Experimental

Cell Biology:

Characterization and Identification of micro-organisms, Karyotyping, Chromosome banding (G & C banding), Phytochemical extraction, Trypan blue cell viability assay

Molecular Biology:

Nucleic acid Isolation (DNA, RNA & plasmid), Gel Electrophoresis, DNA amplification using PCR, PCR (rtPCR & RT-PCR), Cloning, Restriction-digestion-Ligation experiments, Bacterial transformation and selection using Blue-White screening

Protein Study:

Protein extraction and Purification, Affinity chromatography, SDS-PAGE, Western Blotting, ELISA (direct, indirect, sandwich & ODD), Immunoelectrophoresis, Immuno Fluorescence (IF), Enzymatic assays, mass-spectrophotometry

Cell Culture:

Mammalian cell culture, Mammalian cell transfection, MTT & LDH cell viability assays

Histology:

Sectioning, Mounting of mouse tissues, and Staining of mouse tissues

Fermentation:

Setting up a lab scale fermentor, Upstream processing, Downstream processing, Biomass clearance

Structural Biology:

Solving X-Ray crystal structures, Processing the X-Ray diffraction data (iMosfilm, PhaserMR, Molrep) and Structure refinement (Refmac5)

Computational Structural Biology:

Homology modeling, Molecular docking, Molecular dynamic simulations, Essential dynamics, PCA analysis for macromolecular simulation trajectories, Vaccine designing, Epitope mapping, Drug repurposing, Virtual screening, Immunoinformatic analysis, Drug characterization, MM-PBSA studies (Binding free energy calculation), Cross-correlation analysis

Bioinformatics:

Data mining, Detailed study of algorithms, Phylogenetic analysis, Sequence comparison, Primer designing

Data Analysis:

Genomic Analysis:

Whole Genome Assembly (QIIME, VELVET), Metagenomic analysis & Clustering (DESeq, Tophat), Differential gene analysis (Cufflinks), Gene prediction (Genscan), Functional annotation (DAVID)

Programming:

Languages & Statistical Tools:

C, C++, JAVA, Shell Scripting, Python, SQL, Web development, R Statistical Package

Others:

Molecular modeling of secondary & super-secondary protein structures, Molecular modeling of polyproline and collagen models