Nanopore sequencing of nascent RNA

I also had the opportunity to contribute to an interesting nanopore sequencing project led by my colleagues Jenny Vo and Logan Mulroney. Using SMIT I was able to demonstrate that Jenny and Logan’s new approach to enzymatically attach a tail on inosines onto a pool of cellular RNAs in yeast could be used to detect nascent transcripts undergoing transcription. This work was published in the journal RNA (Vo et al. 2021).