Research and Publications
Research Directions
Computational design of novel peptide drugs for the treatments of bacterial infections, inflammation, and cancers.
Nano scale structural studies of hepatitis C virus proteomics.
Bioinformatics of mammalian cell receptors and their interactions with ligands.
Atomic Force Microscopy of Biomedical Systems.
Bio-nano-imaging of native proteins, biomembranes, and living cells.
Biomedical applications of Photovoltaic energy.
Biochemical dynamics of biological membranes.
Protein Chemistry and Proteomics.
Biological Membrane Structure and Biological Membrane Micro-domain Biochemistry.
Skills and expertise
Photocatalysts; Peptide Chemistry; Computational Drug Design; Antimicrobials; Bacteriology; Structural Bioinformatics and Structural Biology; Atomic Force Microscopy and Nano-Imaging; Protein Engineering
Selected Journal Publications:
Gao WY, Chen PY, Hsu HJ, Liou JW, Wu CL, Wu MJ, Yen JH. Xanthohumol, a prenylated chalcone, regulates lipid metabolism by modulating the LXRα/RXR-ANGPTL3-LPL axis in hepatic cell lines and high-fat diet-fed zebrafish models. Biomedicine & Pharmacotherapy. 2024. 174:116598. (SCI).
Hsu PH, Hazam PK, Huang YP, Yeh JC, Chen YR, Li CC, Chang CF*, Liou JW*, Chen JY*. Sequential rearrangement and stereochemical reorganization to design an antimicrobial peptide with enhanced stability. Biomedicine & Pharmacotherapy. 2024. 170: 116088. (SCI).
Hsu PH, Hazam PK, Huang YP, Yeh JC, Chen YR, Li CC, Chang CF*, Liou JW*, Chen JY*. Optimization of sequence and chiral content enhances therapeutic potential of tilapia piscidin peptides. Eur J Med Chem. 2024. 265:116083. (SCI).
Chang CC, Yang CH, Chuang CH, Jiang SJ, Hwang YM, Liou JW*, Hsu HJ*. A peptide derived from interleukin-10 exhibits potential anticancer activity and can facilitate cell targeting of gold nanoparticles loaded with anticancer therapeutics. Communications Chemistry. 2023, 6(1), 278 (SCI).
Chen PY, Lin CY, Wu CL, Keak PY, Liou JW, Gao WY, Lin LI, Yen JH. Pinostrobin modulates FOXO3 expression, nuclear localization, and exerts antileukemic effects in AML cells and zebrafish xenografts. Chemico-Biological Interactions. 2023, 385, 110729 (SCI).
Chang PR, Liou JW, Chen PY, Gao WY, Wu CL, Wu MJ, Yen JH. The Neuroprotective Effects of Flavonoid Fisetin against Corticosterone-Induced Cell Death through Modulation of ERK, p38, and PI3K/Akt/FOXO3a-Dependent Pathways in PC12 Cells. Pharmaceutics. 2023, 15(10): 2376 (SCI).
Mani H, Chang CC, Hsu HJ, Yang CH, Yen JH, Liou JW*. Comparison, Analysis, and Molecular Dynamics Simulations of Structures of a Viral Protein Modeled Using Various Computational Tools. Bioengineering. 2023. 10(9): 1004 (SCI).
Wu IC, Liou JW, Yang CH, Chen JH, Chen KY, Hung CH. Self-assembly of gelatin and collagen in the polyvinyl alcohol substrate and its influence on cell adhesion, proliferation, shape, spreading and differentiation. Front Bioeng Biotechnol. 2023.11:1193849 (SCI).
Lo KM, Lin YS, Liou JW, Chiu TC, Hu CC. Electrochemically synthesized green fluorescent carbon dots for quantitation of hypochlorite and carbendazim. J Food Drug Anal. 2023. 31(2):244-253 (SCI).
Fang ST, Huang SH, Yang CH, Liou JW, Mani H, Chen YC. Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin A. Biomolecules. 2022. 12(12):1799 (SCI)
Chang CC, Hsu HJ, Wu TY, Liou JW*. Computer-aided discovery, design, and investigation of COVID-19 therapeutics. Tzu Chi Med J. 2022. 34(3):276-286 (ESCI, Scopus, PubMed)
Liou JW, Mani H, Yen JH. Viral Hepatitis, Cholesterol Metabolism, and Cholesterol-Lowering Natural Compounds. Int J Mol Sci. 2022 Mar 31;23(7):3897 (SCI)
Mani H., Yen JH, Hsu HJ, Chang CC, Liou JW*. Hepatitis C virus core protein: Not just a nucleocapsid building block, but an immunity and inflammation modulator. Tzu Chi Medical Journal. 2022. 34(2):139-147 (ESCI, Scopus, PubMed)
Chen PY, Gao WY, Liou JW, Lin CY, Wu MJ, Yen JH. Angiopoietin-Like Protein 3 (ANGPTL3) Modulates Lipoprotein Metabolism and Dyslipidemia. Int J Mol Sci. 2021 Jul 7;22(14):7310 (SCI)
Mani H, Chen YC, Chen YK, Liu WL, Lo SY, Lin SH , Liou JW*. Nanosized Particles Assembled by a Recombinant Virus Protein Are Able to Encapsulate Negatively Charged Molecules and Structured RNA. Polymers. 2021 Mar; 13(6): 858 (SCI)
Chen MC, Hsiao YC, Chang CC, Pan SF, Peng CW, Li YT, Liu CD, Liou JW*, Hsu HJ*. Valine-279 Deletion–Mutation on Arginine Vasopressin Receptor 2 Causes Obstruction in G-Protein Binding Site: A Clinical Nephrogenic Diabetes Insipidus Case and Its Sub-Molecular Pathogenic Analysis. Biomedicines. 2021 Mar; 9(3):301 (SCI)
Chang CC, Liou JW, Dass KTP, Li YT, Jiang SJ, Pan SF, Yeh YC, Hsu HJ. Internal water channel formation in CXCR4 is crucial for Gi-protein coupling upon activation by CXCL12. Communications Chemistry. 2020 Oct.; 3: 133. (SCI)
Yang CH, Chen YC, Peng SY, Tsai AP, Lee TJ, Yen JH, Liou JW*. An engineered arginine-rich α-helical antimicrobial peptide exhibits broad-spectrum bactericidal activity against pathogenic bacteria and reduces bacterial infections in mice. Sci Rep. 2018 Oct 2; 8(1):14602. (SCI)
Tseng CC, Yu PY, Liou JW, Chang KC. Altered susceptibility to air sampling stress by filtration is related to colistin resistance development in Acinetobacter baumannii. Indoor Air. 2018 Jun 26. 5: 732-743. (SCI)
Chang CC, Hsu HJ, Yen JH, Lo SY, Liou JW*. A Sequence in the loop domain of hepatitis C virus E2 protein identified in silico as crucial for the selective binding to human CD81. PLoS One. 2017. 12(5):e0177383. (SCI)
Chuang CJ, Liu CD, Patil RA, Wu CC, Chang YC, Peng CW, Chao TK, Liou JW, Liou Y, Ma YR. Impact of cuticle photoluminescence on the color morphism of a male damselfly Ischnura senegalensis (Rambur, 1842). Sci Rep. 2016. 6:38051. (SCI)
Tseng CC, Tsai YH, Hu A, Liou JW, Chang KC, Chang HH. Altered susceptibility to the bactericidal effect of photocatalytic oxidation by TiO2 is related to colistin resistance development in Acinetobacter baumannii. Appl Microbiol Biotechnol. 2016. 100(19):8549-61. (SCI)
Chen HY, Nieh HM, Yang MF, Chou YK, Chung JH, Liou JW. Implementation of a Low-Cost Automated LED Photometer for Enzymatic Reaction Detection to Teach Basic Bioelectronics Technologies in Vocational High Schools. IEEE Transactions on Education. 2016. 59(3): 194-201. (SCI)
Shen CL, Liu CD, You RI, Ching YH, Liang J, Ke L, Chen YL, Chen HC, Hsu HJ, Liou JW, Kieff E, Peng CW. Ribosome Protein L4 is essential for Epstein-Barr Virus Nuclear Antigen 1 function. Proc Natl Acad Sci U S A. 2016. 113(8):2229-34. (SCI)
Jiang SJ, Liou JW, Chang CC, Chung Y, Lin LF, Hsu HJ. Peptides derived from CXCL8 based on in silico analysis inhibit CXCL8 interactions with its receptor CXCR1. Sci Rep. 2015. 5:18638. (SCI)
Hsu SY, Liou JW, Cheng TL, Peng SY, Lin CC, Chu YY, Luo WC, Huang ZK, Jiang SJ. beta-Naphthoflavone protects from peritonitis by reducing TNF-alpha-induced endothelial cell activation. Pharmacol Res. 2015. 102:192-9. (SCI)
Liou JW, Hung YJ, Yang CH, Chen YC. The Antimicrobial Activity of Gramicidin A Is Associated with Hydroxyl Radical Formation. PLoS One. 2015. 10(1):e0117065 (SCI)
Liou JW, Chang FT, Chung Y, Chen WY, Fischer WB, Hsu HJ. In Silico Analysis Reveals Sequential Interactions and Protein Conformational Changes during the Binding of Chemokine CXCL-8 to Its Receptor CXCR1. PLoS One. 2014. 9(4):e94178. (SCI)
Chang KC, Cheng SJ, Chen YC, Huang HR, Liou JW*. Nanoscopic analysis on pH induced morphological changes of flagella in Escherichia coli. J Microbiol Immunol Infect. 2013. 46(6): 405-412. (SCI)
Chang KC, Chiang YW, Yang CH, Liou JW*. Atomic force microscopy in biology and biomedicine. Tzu Chi Med J. 2012. 24(4): 162-169. (ESCI, Scopus)
Liou JW, Chang HH. Bactericidal effects and mechanisms of visible light-responsive titanium dioxide photocatalysts on pathogenic bacteria. Arch Immunol Ther Exp (Warsz). 2012. 60(4):267-75. (SCI)
Liou JW, Gu MH, Chen YK, Chen WY, Chen YC, Tseng YH, Hung YJ, Chang HH. Visible light responsive photocatalyst induces progressive and apical-terminus preferential damages on Escherichia coli surfaces. PLoS One. 2011. 6(5):e19982. (SCI)
Kumar VB, Yuan TC, Liou JW, Yang CJ, Sung PJ, Weng CF. Antroquinonol inhibits NSCLC proliferation by altering PI3K/mTOR proteins and miRNA expression profiles. Mutat Res. 2011. 707(1-2):42-52. (SCI)
Chan SC, Lo SY, Liou JW, Lin MC, Syu CL, Lai MJ, Chen YC, Li HC. Visualization of the structures of the hepatitis C virus replication complex. Biochem Biophys Res Commun. 2011. 404(1):574-8. (SCI)
Liou JW, Mulet X, Klug DR. Absolute measurement of phosphorylation levels in a biological membrane using atomic force microscopy: the creation of phosphorylation maps. Biochemistry. 2002. 41(27):8535-9. (SCI)
Selected Conference Publications:
Yang, CH, Chang, FC, Tsai, SL, Cheng, MF, Jiang, SJ, Liou, JW. Finding physical characteristic rules of peptides against different target microbes. 2013/Jun. 23rd American Peptide Symposium, Hilton Waikoloa Village, Hawaii, USA. P180.
Lai, PM, Liou, JW. de novo design of antimicrobial and anticancer peptides. 2013/Jun. The 18th Biophysics Conference, Academia Sinica, Taipei, Taiwan. p.78/P2-003.
Lian, YJ, Liou, JW. The relationships between peptide structures and hydroxyl radical productions during peptide bactericidal and tumoricidal treatments. 2013/Jun. The 18th Biophysics Conference, Academia Sinica, Taipei, Taiwan. p.86/P3-002.
Chen, WY, Liou, JW, Hsu, HJ. In silico investigations of possible therapeutic peptides as drugs for anti-inflammatory responses. 2013/Feb. Biophysical Society 57th annual meeting, San Francisco, California, USA. p.129/2062-Pos.
Chen, YK, Liou, JW. Hepatitis C Virus core protein secondary structure changes induced by protein/nucleic acid interactions. 2012/May. The 17th Biophysics Conference, Academia Sinica, Taipei, Taiwan. p.110/P4-014.
Liu, WL, Liou, JW. Interactions between hepatitis C virus core protein and negatively charged molecules. 2011/May. The 16th Biophysics Conference, National Dong Hwa University, Haulien, Taiwan. p.83/P1-029.
Huang, HR, Liou, JW. The relationship between alpha-helix contents of antimicronial peptide and hydroxyl radical productions during bactericidal treatments. 2011/May. The 16th Biophysics Conference, National Dong Hwa University, Haulien, Taiwan. p.68/P1-011.
Yang, CH, Liou, JW. Design, synthesis, and characterization of antimicrobial peptides against Staphylococcus aureus. 2011/Jan. 7th Asian Biophysical Association Symposium, New Delhi, India. p.64/O-12.
Jung, JL, Lin, SH, Chen, YC, Liou, JW, Structural analysis of hepatitis C virus (HCV) core assembly and HCV core-lipid interactions. 2010/May. The 15th Joint Biophysics Conference, Academia Sinica, Taipei, Taiwan. p.P1-014.