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One of the potential modes of dissemination of this pathogen in the environment is by insects that are associated with animal feces and manure, primarily houseflies (HF; Musca domestica L.). HF larvae develop in animal feces, including cattle manure. Consequently, HF commonly build up very large populations on cattle farms and in other animal facilities. Previously, a laboratory-based study demonstrated that E. coli O157:H7 ingested by HF remained viable in the fly excreta and that the HF were able to carry and disseminate E. coli for several days (9). In Japan, HF were implicated in the transmission of E. coli O157:H7 from reservoir animals to other animals and humans (10).

The objectives of this study were (i) to assess the temporal prevalence and concentration of E. coli O157:H7 associated with HF collected on a beef cattle farm, (ii) to characterize E. coli O157:H7 isolates by screening for the virulence factors genes and antibiotic resistance, and (iii) to determine the concentration of fecal coliforms carried by HF in the cattle farm environment as an indicator of fecal contamination.

HF were collected by sweep net from two sites (feed bunks and a storage shed containing steam-flaked corn) on a cattle feedlot in northeastern Kansas four times per week from mid-June until the end of October 2003. From each collection, 50 HF were randomly selected and processed for E. coli O157 isolation and quantification. Individual HF (n = 3,440) were homogenized in 1 ml of phosphate-buffered saline (pH 7.2; ICN Biomedicals) and serially diluted. Dilutions were plated by a direct drop plate technique onto sorbitol MacConkey agar (Becton Dickinson) with cefixime (25 g/liter) and tellurite (1.25 mg/liter). Plates were incubated overnight at 37C, and sorbitol-negative colonies with a morphology characteristic of E. coli O157 were tested for the O157 antigen by the latex agglutination assay (Oxoid Limited, Basingstoke, England). Colonies positive for the O157 serogroup were counted, and up to three positive colonies per sample were subcultured on Trypticase soy agar (Becton Dickinson) and identified by the API Rapid 20E test (Biomerieux Vitek). Isolated E. coli O157 strains were tested for the virulence genes (stx1, stx2, and eaeA) and the flagellar H7 gene (fliC) by PCR as described previously (4, 6). The sensitivity of E. coli O157:H7 isolates (n = 108) to 10 antibiotics (ampicillin, apramycin, ceftiofur, chlortetracycline, enrofloxacin, gentamicin, neomycin, oxytetracycline, spectinomycin, and trimethoprim-sulfamethoxazole) was measured by determination of the MIC in accordance with the National Committee for Clinical Laboratory Standards (NCCLS) guidelines (12) with a diagnostic system (TREK Diagnostic Systems, Cleveland, Ohio) with the Sensititre AutoInoculator, sensitivity antibiotic plates, and the SensiTouch plate reader. Fecal coliform counts in randomly selected individual fly homogenates (n = 350) were determined by a drop plate technique with mFC (membrane fecal coliform) agar (Oxoid Limited) incubated at 44.5C for 18 to 24 h. Fecal coliform colonies were counted from dilutions and calculated per fly.

The very large populations, suitable habitats (feces, manure), unrestricted movement, and mode of feeding (regurgitation) of HF represent a likely mechanism for the spread of E. coli O157:H7 on cattle farms and into the surrounding environment. The infectious dose of E. coli O157:H7 is very low, probably fewer than 100 cells (17). Humans can become infected through the consumption of contaminated food, particularly inadequately cooked ground beef (often in the form of beef burgers) and milk (nonpasteurized or contaminated postpasteurization). Other sources of infection include contaminated nonpasteurized apple cider, water (drinking and swimming), vegetables, mayonnaise, lamb, venison, deer jerky, cured salami, and direct contact (animal to person or person to person) (2, 15, 16). Because of the attraction of HF to places where food is prepared, stored, and consumed, these insects present a potential route for the contamination of food and drinks. The dispersal range of HF is usually 0.5 to 2 miles, although distances as great as 10 to 20 miles have been reported (3). Human infections associated with E. coli O157:H7 are more common during summer months, with the majority of cases occurring from May to September. This correlates with an increased shedding of E. coli O157:H7 by cattle, as well as with very large populations of HF on cattle farms and in other animal operations.

A beloved 100-year-old cattle farm in Livingston, Michigan, has ceased operations after state officials found PFAS pollution contaminating the grains, groundwater, and livestock. VICE recently covered the story, showcasing how a serial polluter devastated the livelihood of a Michigan family. The report also includes the Ecology Center's research on PFAS in sewage sludge made from biosolids, showing widespread PFAS contamination in sewage sludge.

We don't buy it either. In 2021, the Ecology Center and the Sierra Club looked at PFAS in fertilizer products made from sewage sludge. We found these "forever chemicals" in all nine products tested. Maine has already taken action by banning the spreading of sewage sludge as farm fertilizer due to PFAS contamination in 2022.

Though the state has been helping with feed costs and buying some cattle ready for market (to send to a landfill), the Grostic's farm has been left with fields full of PFAS, no income, cattle still needing tending, and no answers, as experts don't know how to remediate the soil.

Alongside the Great Lakes PFAS Action Network, the Ecology Center encourages the state to develop a financial safety net for farmers affected by PFAS in their water or soils. Other states, like Maine, have created funds to help farmers lessen the load of the financial burdens when PFAS are discovered. Our policy agenda for Michigan and the Great Lakes region outlines further steps needed to turn off the tap on PFAS for good.

The primary research emphases at the Beef and Sheep Research Field Laboratory are applied nutrition genetics, physiology, lactation, production, and management. Eight cattle barns and one sheep barn house 1,000 beef cattle (800 feedlot cattle and about 200 purebred Angus) and 100 sheep. This state-of-the-art facility features a GrowSafe water intake and body weight monitoring system. It also includes a metabolism lab, and Ruminant Emissions Measurement System (REMS) that measures greenhouse gas production.

We do not compromise our progress to compare against other farms. We firmly believe our differentiating factor which sets us apart is found in the flavor of our beef. As a family who has lived here our entire lives, we make it our eager ambition and passion to provide locally raised cattle for our community.


When we make the statement that our farm is "family owned and operated", we mean it. My Husband and I work the farm with the help of our in-laws who live just a few hundred feet across the pasture. This farm is our pride and joy and we hope you enjoy the fruits of our labors as much as we do!

The Beef Teaching Farm strives for excellence in cattle production and hands-on teaching. The herd has both spring and fall calving programs. Purebred calves are born in the spring - primarily March and April. Angus and Simmental breeds comprise the purebred herds.

The surplus spring calves and fall born crossbred calves are fed retained ownership at the Ames unit. The cattle are used in 10 undergraduate classes, judging contests, ICA events and competitions, ultrasound training and certification, artificial insemination schools, and other beef promotions and educational training purposes.

Dairy cows are all female because the industry requires them to give birth and produce milk for their babies. That milk is then taken to be marketed for consumption by humans. Cattle farmers specifically breed dairy cows to produce large quantities of milk. In the U.S., the Holstein Friesian and Jersey breeds are usually used on dairy farms.

Beef cattle are bulls and calves raised to be killed for meat. Many of the calves are turned into veal, by being killed at 2 or 3 days old, and sometimes even at just 2 or 3 hours. The rest are raised to be fattened for beef. Just as with dairy cows, beef cattle are selectively bred, to help produce and sell different commodities, such as leaner meats. Most beef cattle in the U.S. are eventually put in enclosures in feedlots before slaughter, where they survive in unsanitary conditions, while only a small percentage are given continuous access to pasture.

Often thought of as a byproduct of the dairy and meat industries, leather has in fact become one of the main sources of profit for cattle farmers. Leather is the skin of a cow, bull or calf. It is usually young calves that are killed, for their soft and unmarked skin.

Cattle farms are often known as either beef farms or dairy farms, depending on the purpose for which the cows are being raised. The majority of cows are also raised in large, intensive farms that are classed by the Environmental Protection Agency as Concentrated Animal Feeding Operations or CAFOs, and which are commonly called factory farms.

How cattle farms are maintained differs depending on what kind of cattle farm it is. Dairy farms require milking equipment, for example, while the farms that buy mature beef cows to ready them for slaughter require feedlots where cattle are fed grains to fatten them up. Some important aspects of running and maintaining a cattle farm include handling, grazing, housing, fly control and reproduction.

Cows need to be handled in a way that prevents stress or injury, and that ensures their overall well-being. But quite the opposite tends to be the case in cattle farms. Cows are often stationed on concrete floors for a long period, damaging their hooves and causing sore joints. Many dairy cows are milked using machines, which among other things cause mastitis, leading to pus in milk. Cattle handling also involves literally breaking up families, by separating lactating cows from their babies, so that the milk can be used for dairy and the babies can be used as meat. 5376163bf9

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