Physio- or Patho-physiolocal functional studies of ion channels using interactomes based on PPIs in the brain

• Physiological functions of ion channels in the brain

- TREK-1 channel mediates fast glutamate release in astrocytes upon GPCR activation (2012, Cell)

- TREK-1 makes a heterodimer with TWIK-1 and mediates passive conductance in astrocytes (2014, Nature Comm.)

- TWIK-1 contributes to the intrinsic excitability of dentate gyrus granule cells (2014, Mol Brain)

- TWIK-1/TASK-3 heterodimeric channels contribute to the neurotensin-mediated excitation of hippocampal dentate gyrus granule cells (2018, Exp Mol Med)

- TTYH1 and TTYH2 Serve as LRRC8A-Independent Volume-Regulated Anion Channels in Cancer Cells (2019, Cells)

- Spadin Modulates Astrocytic Passive Conductance via Inhibition of TWIK-1/TREK-1 Heterodimeric Channels (2021, IJMS)

• Pathophysiological functions of ion channels in the brain

- GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's disease (2014, Nat Med.)

- Depletion of 14-3-3 gamma attenuates TRPM4-mediated glutamate-induced neuronal cell death (2014, Mol Brain)

- Suppression of 14-3-3 gamma-mediated surface expression of ANO1 inhibits cancer progression of glioblastoma cells (2016, Sci rep.)

- The Knockdown of TREK-1 in Hippocampal Neurons Attenuate Lipopolysaccharide-Induced Depressive-Like Behavior in Mice (2019, Int J Mol Sci.)

- Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression (2020, Cells)

- TMEM16A expression in cholinergic neurons of the medial habenula mediates anxiety-related behaviors (2020, EMBO Rep.)

• Development of molecular tools for controlling the cell-type specific gene expression and optogenetic stimulation

- Optogenetic Inactivation of the subthalamic nucleus improves forelimb akinesia in a rat model in parkinson disease (2014, Neurosurgery)

- Neuronal expression and cell-type specific gene-silencing of BEST1 in thalamic reticular neucleus neurons using pSico-Red system (2016, Exp Neu.)

- Optogenetic retinal prosthesis system (국내 특허등록, 2016 Feb)

- Improved AAV vector system for cell-type-specific RNA interference (Addgene donation: 2022, J Neuro Methods)

- Caspase-dependent apoptosis induces reactivation and gliogenesis of astrocytes in adult mice (TaCasp-TG, 2022, Front Cell Neurosci.)