P 08 - Advanced GC Approaches for Identification of Unusual Bacterial Fatty acids.
P 08 - Advanced GC Approaches for Identification of Unusual Bacterial Fatty acids.
The determination of the short-chain fatty acids (SCFAs) in biological fluids has gained much attention in the last decades because of their relevant implication as fermentation end products of gut microbiota [1]. Acetic (C2), propionic (C3), and butyric (C4) fatty acids are among the most abundant SCFAs in biological fluids, and their amount can be monitored in order to assess the health state of patients. From an analytical point of view, different approaches have been used to determine SCFAs in biological fluids. The extraction of the SCFA compounds from human plasma is conventionally performed using organic solvent [2].
The goal of this study is to develop a fast and reliable gas chromatography-mass spectrometry GC-MS) method for the measurement of short-chain fatty acids (SCFAs) in biological fluids. The extraction procedure involved the use of acidified water and methyl tert-butyl ether (MTBE). The separation of the analytes was performed using a dedicate narrow-bore GC capillary column of dimensions 30 m × 0.25 mm ID × 0.25 μm df with acid-modified poly(ethylene glycol) stationary phase. The acquisition of signals was performed in single ion monitorin (SIM) mode. The novelty of the research involves reducing the analysis time (less than 7 min) by using the novel fast GC–MS method.
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R. García-Villalba, J.A. Gimenez-Bastida, et al. J Sep Sci. 35 (15) (2012) 1906–1913, https://doi.org/10.1002/jssc.201101121.