Embedded Files
Objectives
Objectives
The Department of Biotechnology at IIT Madras is organizing the first-of-its-kind workshop in India on:
1) Förster Resonance Energy Transfer (FRET)
2) Fluorescence Lifetime Imaging Microscopy (FLIM)
3) Fluorescence Correlation Spectroscopy (FCS)
4) Fluorescence Recovery After Photobleaching (FRAP)
Hands-on instructions on Olympus wide-field and confocal microscopy systems
Internationally recognized faculty and microscopy support specialists
Best imaging and analysis solutions
Individual problem solving, discussion and trouble-shooting
Free access to training toolkit, reference guides, and image analysis software.
ORGANIZING COMMITTEE
ORGANIZING COMMITTEE
Dr. Shantanu Pradhan
Dept. of Biotechnology
IIT Madras
Dr. Ammasi Periasamy
Director and Founder, Keck Center for Cellular Imaging, University of Virginia, USA
Dr. Amal Kanti Bera
Dept. of Biotechnology
IIT Madras
Dr. Ganesh Kadasoor
Senior Application Scientist,
Evident Scientific India
Dr. Sebastian Raja
Applications Manager, DSS Imagetech India
Dr. Yuansheng Sun
Director of Microscopy & Spectroscopy,
ISS Inc., USA
SPEAKERS & TRAINERS
SPEAKERS & TRAINERS
Dr. Ammasi Periasamy
Director and Founder, Keck Center for Cellular Imaging, University of Virginia, USA
Dr. Amal Kanti Bera
Professor, Dept. of Biotechnology
IIT Madras, Chennai, India
Dr. Ganesh Kadasoor
Senior Application Scientist,
Evident Scientific India
Dr. Sebastian Raja
Applications Manager, DSS Imagetech India
Dr. Sudipta Maiti
Professor and Chair,
Dept. of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai, India
Dr. Michael Börsch
Head - Microscopy Methodology Research Group
University Hospital Jena, Germany
Dr. Krishanu Ray
Associate Professor, Institute of Human Virology, University of Maryland, USA
Dr. Huiwang Ai
Professor of Molecular Physiology & Biophysics, Chemistry, and Biomedical Engineering, University of Virginia, USA
Dr. Klaus Suhling
Professor, Dept. of Physics, King's College London, UK
Dr. Shagufta Rehman Alam
Senior Scientist,
Keck Center for Cellular Imaging, University of Virginia, USA
Dr. Margarida M Barroso
Professor,
Molecular & Cellular Physiology, Albany Medical College, USA
Dr. Wolfgang Becker
President, Becker & Hickl GmbH, Germany
INSTRUMENTS & TECHNIQUES
INSTRUMENTS & TECHNIQUES
Olympus FV3000 laser scanning confocal microscope
Olympus IX83 wide-field camera-based microscope
ISS Inc. – FLIM, FLIM-FRET, FCS modules
Confocal/spectral FRET
FCS
FLIM-FRET
Imaging live/fixed cells
Anisotropy / Homo-FRET
FLIM analysis: Fitting and Phasor plots
FLIM-FRET Microscopy to investigate proteins dimerizing in the live cell nucleus. Nature Protocols 6 (9), 1324-1340, 2011
Comparing uFRET (uncorrected for spectral bleed-through - SBT) with PFRET (Processed FRET, corrected for SBT)
Label-free NADH fluorescence lifetime imaging and quantitative analysis by phasor plots
Fluorescence Correlation Spectroscopy (FCS)
Binding of mAb F240, A32, C11, b12 and Synagis to intact AT-2–inactivated HIV-1 BaL virion as determined by FCS. Sci Rep. 2016; 6: 36685
ABSTRACT SUBMISSION
ABSTRACT SUBMISSION
REGISTRATION
REGISTRATION
Registration deadline: CLOSED
Registration link: https://elearn.nptel.ac.in/F4ABW2023/
Fees: IIT Madras participants - Rs. 5000/- (+ GST), External participants – Rs. 8000/- (+ GST) (includes food, accommodation, and workshop materials)
Participation certificates will be provided. Participation is limited to 15 students/postdocs/young faculty.
PARTNERS
PARTNERS
IIT Madras Global Engagement Office
IITM Centre for Outreach & Digital Education
Evident Scientific India
ISS Inc.
DSS Imagetech
CONTACT
CONTACT
Email: advancedbioimagingworkshop2023@gmail.com
Address: Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences,
Indian Institute of Technology Madras, Chennai 600036
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