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The overall objective of the research is to evaluate how environmental (e)DNA and other DNA-based methods can complement and support traditional monitoring methods for the Water Framework Directive (WFD) across a range of biological quality elements and for other regulatory objectives related to biodiversity in rivers.
Moreover, we will outline the added values to be gained from using DNA-based approaches, including potential new DNA metrics, and provide guidance for their integration into existing programmes.
Our aim is to develop methods for the rapid generation of DNA barcoding protocols to supplement sequence archives with Irish specimens and to evaluate the possibility of using DNA methods for macrophyte diversity and species identification.
https://www.synaptiq.ai
Macroinvertebrates
Macroinvertebrates
We are looking to compare DNA estimates of species diversity, Q-values and taxa abundances obtained from metabarcoding of water and bulk samples with those from traditional methods (e.g., kick-sampling) for freshwater invertebrates.
https://www.mccrone.com
Diatoms
Diatoms
Our purpose is to compare the assemblage composition from traditional and DNA-based analyses of biofilm samples, thus developing new metrics optimised for DNA-based data and evaluating them against key pressure gradients.
https://www.fisheriesireland.ie
Fish
Fish
By implementing DNA metabarcoding methods to assess the presence of fish species, we aim to test the possibility of quantifying fish in rivers by comparing DNA with existing field data. In addition, we will model the cost/benefit ratio of DNA compared to traditional methods.
https://fishhealthforum.com
Microbes
Microbes
We plan to use DNA metabarcoding to trace microbial sources in freshwater sites, comparing the results of traditional and genetic methods.
https://www.thoughtco.com
We aspire to exploit DNA technologies to produce new sequence data on Irish freshwater species that will fill gaps in existing biodiversity archives (https://freshbase.myspecies.info, BOLD , and Genbank).
https://www.biologyonline.com
The ambition is to test the generated sequence data for the presence of anthropogenic inputs, such as the presence of wastewater, sewage and other agricultural runoff in the water, using DNA to trace sources of contamination within sub-catchments.
The ambition is to test the generated sequence data for the presence of anthropogenic inputs, such as the presence of wastewater, sewage and other agricultural runoff in the water, using DNA to trace sources of contamination within sub-catchments.
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