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We explore and manipulate the light-matter interaction of molecules. Our recent research activities focus on studying and developing biomolecules and methods for biomedical imaging and sensing applications.
We explore and manipulate the light-matter interaction of molecules. Our recent research activities focus on studying and developing biomolecules and methods for biomedical imaging and sensing applications.
Selected Research Projects
Selected Research Projects
Genetically encodable fluorescent probes
Genetically encodable fluorescent probes
Fluorescence microscopy is a major approach to investigating biological systems due to its non-invasive detection, sensitivity and imaging flexibility. Super-resolution microscopy (nanoscopy) further breaks the Abbe diffraction limit offering the unprecedented spatial resolution that was only achievable with electron microscopy previously. A critical requirement in the development and applications of nanoscopy is proper molecular probes. Fluorescent proteins (FPs) are an essential category of such molecular probes owing to their unique genetic encodability and the ability to be modulated by light. We are developing a rare type FPs suitable for nanoscopy through directed evolution.
PCCP 24, 14310 (2022)
J Phys Chem B 126, 4659 (2022)
Biochem 59, 3669 (2020)
Integr Biol 10, 516 (2018)
Cell isolation
Cell isolation
Cell sorting platforms are essential tools for the development of biomedicine and biotechnology. Isolation of cells can be achieved with various methods, such as flow cytometers, microfluidic sorters and imaging cytometers. We develop a variety of cell screening and sorting platforms for various requirements and purposes of research.
Lab Chip 20, 834 (2020)
Metal enhanced fluorescence
Metal enhanced fluorescence
Fluorescence is an essential detection technique in medical diagnostics and biotechnology. It is challenging to balance fluorescence signals and the photostability of fluorescent tags in fluorescence imaging and sensing. Metal-Enhanced Fluorescence (MEF), demonstrated by K. H. Drexhage in the 1970s, primarily utilizes the coupling between plasmons in metal nanostructures and fluorophores to enhance their fluorescence. MEF simultaneously enhances fluorescence intensity and photostability and thus is advantageous in bioimaging and sensing. We are investigating the mechanism of MEF with a novel approach using the two-photon fluorescence technique. We are also developing MEF sensing methods to lower the detection limit in regular fluorescence detection.
Raman spectroscopy study for reversibly switchable fluorescent proteins
Raman spectroscopy study for reversibly switchable fluorescent proteins
Fluorescent proteins (FPs) are indispensable tools in biological and biomedical research owing to their genetic encodability and light-modulation capability. Reversibly switchable FPs play an important role in super-resolution microscopy such as Reversible Saturable Optical Fluorescence Transition microscopy (RESOLFT), due to their photo-induced cis-trans isomerization. However, the broad fluorescence bandwidth hampers FPs’ applications in multi-color imaging. In contrast, stimulated Raman spectroscopy (SRS) possesses narrow bandwidths and thus is advantageous for multiplex super-resolution imaging techniques. This work explores Raman signals of FPs under various excitation conditions for potential super-resolution SRS microscopy applications.
Biosensing chip
Biosensing chip
Pancreatic cancer is the twelfth most common cancer globally and the prognosis five-year survival rate remains low due to the difficulty in diagnosis at the early stage. We are developing an electronic biosensing chip and exploring various biomarkers to achieve the early detection of pancreatic cancer.
IEEE Sens. J. 24, 31754 (2024)
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