Measures native molecular weight and in some cases Kd of binding/oligomerization

Provides information on native molecular weights of species in solutions

Can read wavelengths from 250 nm - 800 nm and refractive index

Requires 400-600 ul of material at concentrations sutiable for detection by absorbance (0.1-1 mg/ml for proteins, less for nucleic acids and molecules with fluorophores)

Temperature from 4C - 37C

Measurements take 17-24 hours

Not for purification (analysis only)

Measure native MW of a single or multiple species in solution

Requires only 1 drop

Measurement takes minutes

Determine Stoke's radius of species in solution 

Can relate measurement to native molecular weight

Can determine native MW of different species in solution

Requires about 10 ul of sample at concentrations ~1 mg/ml

Measurement takes minutes

Lyses cells quickly and easily

Minimum volume 10 ml

No maximum volume

Lyse by passing cells through instrument 3-4 times. Each 250 ml of cell culture takes about 15 minutes