Holly Rucker | Researcher

Nitrogen fixation

Publications & Preprints:

★ Persistent nitrogenase isotope signatures over two billion years of engineered evolution

★ Enigmatic evolution of microbial nitrogen fixation: insights from Earth’s past

★ Ancient nitrogenases are ATP dependent

★ Emergence of an orphan nitrogenase protein following atmospheric oxygenation

★ Ecological resource competition as a driver of metallome evolution

Conducted at the University of Wisconsin-Madison

My research interests include:

Ancestral sequence reconstruction and resurrection

Precambrian geobiology & the co-evolution of the bio- and geospheres

Stable isotopes to understand microbial metabolism

Microbial genome engineering & synthetic biology

Previous research:

Pheromone synthesis

★ Decreased attractivity in female garter snakes treated with an aromatase inhibitor

★ Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
★ Reconsidering reproductive patterns in a model dissociated species, the red-sided garter snake

Conducted at James Madison University

Bioenergetics of hydrothermal vents

★ Quantifying the Bioavailable Energy in an Ancient Hydrothermal Vent on Mars and a Modern Earth-Based Analog

Conducted at Stony Brook University

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