遠紅光光合作用的應用

FaRLiP 藍綠菌

藍綠菌的FaRLiP可以有什麼應用？有可能將其轉移使其他物種利用遠紅光嗎? 我們致力於將FaRLiP的基因與機制運用於合成生物學的研究當中，包含但不限於尋找可以受遠紅光調控的啟動子以及在藍綠菌甚至是其他細菌或生物建立可以用遠紅光調控的系統

相關發表與文獻:

Liu T-S*, Wu K-F*, Jiang H-W, Chen K-W, Nien T-S, Bryant DA, Ho M-Y. (2023) Identification of a far-red light-inducible promoter that exhibits light intensity dependency and reversibility in a cyanobacterium. ACS Synth. Biol. 12(4), 1320–1330. (*co-first author) https://doi.org/10.1021/acssynbio.3c00066
Tabor JJ, Levskaya A, & Voigt CA. (2011) Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405(2), 315–324. https://doi.org/10.1016/j.jmb.2010.10.038
Hirose Y, Shimada T, Narikawa R, Katayama M, & Ikeuchi M. (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc. Natl. Acad. Sci. U.S.A. 105(28), 9528–9533. https://doi.org/10.1073/pnas.0801826105

我們分析FaRLiP藍綠菌株在遠紅光下的轉錄組，鑑定出遠紅光誘導表現的基因。透過我們建立的螢光蛋白報導系統，測試這些基因上游可能的啟動子序列。其中PchlFJSC1等啟動子會顯著地被遠紅光誘導。

相關發表與文獻:

Liu T-S*, Wu K-F*, Jiang H-W, Chen K-W, Nien T-S, Bryant DA, Ho M-Y. (2023) Identification of a far-red light-inducible promoter that exhibits light intensity dependency and reversibility in a cyanobacterium. ACS Synth. Biol. 12(4), 1320–1330. (*co-first author) https://doi.org/10.1021/acssynbio.3c00066
Kobayashi S, Atsumi S, Ikebukuro K, Sode K, & Asano R. (2022) Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria. Microb. Cell Factories. 21(1), 7. https://doi.org/10.1186/s12934-021-01732-x

透過篩選出的遠紅光調控啟動子，我們可以結合調控FaRLiP gene cluster表現的調節子rpfABC，使藍綠菌在遠紅光下表現eYPF，並在沒有光照的情況下關閉該表現系統。我們目前也致力於將這套系統運用在其他不能使用遠紅光的藍綠菌或是其他生物中。

相關發表與文獻:

Liu T-S*, Wu K-F*, Jiang H-W, Chen K-W, Nien T-S, Bryant DA, Ho M-Y. (2023) Identification of a far-red light-inducible promoter that exhibits light intensity dependency and reversibility in a cyanobacterium. ACS Synth. Biol. 12(4), 1320–1330. (*co-first author) https://doi.org/10.1021/acssynbio.3c00066
Sarsekeyeva F, Zayadan Bk, Usserbaeva A, Bedbenov VS, Sinetova MA, & Los DA. (2015) Cyanofuels: biofuels from cyanobacteria. Reality and perspectives. Photosynth. Res. 125(1-2), 329–340. https://doi.org/10.1007/s11120-015-0103-3
Colin MB, Léda NG, Deborah LS, Mark EH, Stephen CM, Michael JW, Jefferson WT, Ian A, Joe G, Charles HG. (2015) Algal biofuel production for fuels and feed in a 100-ha facility: A comprehensive techno-economic analysis and life cycle assessment. Algal Res. 10, 266-279. https://www.sciencedirect.com/science/article/pii/S2211926415001125

藍綠菌廣泛運用於生質能源或化合物合成等應用，生長於生物反應器中可能會隨著細胞濃度上升而出現光照不足等問題。透過讓細胞具備利用遠紅光行光合作用的能力，將有機會改善生物反應器的光照問題，增加反應器可使用的場域。

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