Research Title: In the synthesis of bioethanol from banana, what contributes greatest to the consequent yield of bioethanol, the concentration of amylase supplied during enzymatic pre-treatment or that of yeast catalysing the fermentation process?
Type: Extended Research Thesis/Independent Research Project/Peer-Reviewed Publication
Research Supervisor: Rachel Ingram, Head of Chemistry Department - United World College Dover
Abstract:
Given my background in farming and agriculture I was particularly interested in the role that biofuels could potentially play in mitigating the effects of climate change and reducing our reliance on fossil fuels. I wanted to investigate the role enzymes played in the synthesis of biofuel from biomass and whether amylase supplied during enzymatic pre-treatment, or yeast catalysing the fermentation process had a prominence over the other in the consequent bioethanol yield produced. Three different fermentation batch samples were categorized based on the enzyme compositions that they would receive. The samples of the fermentation batch samples were then subjected to enzymatic pre-treatment by addition of their respective compositions of amylase and yeast for a period of 15 minutes. Making use of a standardised glucose calibration curve, the glucose concentrations of the varying samples of the fermentation batches of banana post enzymatic pre-treatment was determined. This provided preliminary evidence of amylase having greater prominence than yeast in producing a greater bioethanol yield. Since the more glucose present the more was available for fermentation. Afterwards the banana samples were sealed within conical flasks and submerged within water baths, with optimal conditions for fermentation to take place for a period of 3 days. The samples were then removed and suspended over acidified potassium dichromate and were left overnight to undergo oxidation. The bioethanol concentration of the fermentation batch samples was then determined by performing a titration against the varying degrees of the oxidised dichromate.
Experimental results for the bioethanol concentration of the fermentation batch samples indicated that amylase when supplied independently vs yeast resulted in greater bioethanol yields and concentrations. However, for the samples where both amylase and yeast were added concurrently the results demonstrated that the optimal enzyme composition for maximising the yield and concentration of the bioethanol was a 3:2 proportion of amylase vs yeast. The conjugate proportion of 2:3 however, still produced a greater yield than the samples where the proportion of enzyme added was entirely amylase. Which indicates that yeast is essential to producing bioethanol from biomass since it catalyses the fermentation process. However, when added concurrently with amylase its proportion is not as significant in producing the consequent bioethanol yield than amylase. This provides some insight into what factors industrial engineers would need to take into consideration when mass producing bioethanol on the industrial scale, the optimal enzyme composition with respect to cost and the bioethanol yield.