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CSEMs (conventional SEMs with a thermic electron source) and FE-SEMs (field emission SEMs with a field emission electron source) from ZEISS deliver high resolution imaging and superior materials contrast.
Workshops, One-on-One Training, and Equipment Demos - ranging from a general introduction to the field of electron microscopy to very specific techniques. Located in Hatfield, PA, our Academy houses several laboratories allowing students to get hands-on training in SEM, TEM and Wet Labs with state-of-the-art equipment. Sign up today!
Updated for 2023! We look forward to meeting with our customers in person or in virtual environments. We welcome you to visit us at these tradeshows throughout the year. Find out how we're about electron and all microscopy sciences!
Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in biomedical research to investigate the detailed structure of tissues, cells, organelles and macromolecular complexes. The high resolution of EM images results from the use of electrons (which have very short wavelengths) as the source of illuminating radiation. Electron microscopy is used in conjunction with a variety of ancillary techniques (e.g. thin sectioning, immuno-labeling, negative staining) to answer specific questions. EM images provide key information on the structural basis of cell function and of cell disease.
Conventional scanning electron microscopy depends on the emission of secondary electrons from the surface of a specimen. Because of its great depth of focus, a scanning electron microscope is the EM analog of a stereo light microscope. It provides detailed images of the surfaces of cells and whole organisms that are not possible by TEM. It can also be used for particle counting and size determination, and for process control. It is termed a scanning electron microscope because the image is formed by scanning a focused electron beam onto the surface of the specimen in a raster pattern. The interaction of the primary electron beam with the atoms near the surface causes the emission of particles at each point in the raster (e.g., low energy secondary electrons, high energy back scatter electrons, X-rays and even photons). These can be collected with a variety of detectors, and their relative number translated to brightness at each equivalent point on a cathode ray tube. Because the size of the raster at the specimen is much smaller than the viewing screen of the CRT, the final picture is a magnified image of the specimen. Appropriately equipped SEMs (with secondary, backscatter and X-ray detectors) can be used to study the topography and atomic composition of specimens, and also, for example, the surface distribution of immuno-labels.
A very nice scanning electron microscope (SEM) plus a focused ion beam (FIB).
The EM Facility is an institutional resource to meet the electron microscopy needs of faculty, staff and students of the Dartmouth College community and beyond.
Understanding the microscopic structure of materials is essential for determining their properties and for the creation of new, useful devices. For decades, electron and X-ray microscopies have been used to look inside matter. Electron microscopes can now resolve single atoms buried within structures, while X-ray microscopes can discern minute lattice distortions in materials. Center for Nanoscale Materials (CNM) researchers with deep expertise in these two areas work closely together to create the most powerful images of material structures and dynamics.
The University of Missouri is home to the most advanced electron microscopy core facility in the Midwest (see our 2021 press release). Located in the Roy Blunt NextGen Precision Health building, the Electron Microscopy Core (EMC) houses a world-class suite of instrumentation capable of addressing research questions across virtually all fields in both materials and life sciences. The EMC serves investigators from academia and industry and offers assistance from project design through execution and delivery.
The generating activity will determine if the electron tubes to be transferred to the DLA Disposition Services site contain radioactive material (radioactivity) in amounts less than, equal to, or in excess of the radioactivity, or qualify as unimportant.
Radioactive material in some electron tubes may not be detectable by radiological survey meters and may only be identified by consulting information sources such as MSDS, HMIRS, Federal Logistics Information System (FLIS), and the product manufacturer.
The DLA Disposition Services site will not accept physical custody of electron tubes that have non-detectable levels of radiation based on radiological survey meters when conflicting data from MSDSs, HMIRS, FLIS, or the manufacturer indicates there is any amount of radioactive material in the electron tube.
Following the determination that an electron tube is non-radioactive generating activities need to be aware that electron tubes may still contain hazardous components such as beryllium containing ceramics, lead, cadmium, mercury, and other regulated substances.
The generating activities will determine if the electron tubes to be physically turned in to the DLA Disposition Services site contain any regulated hazardous substances, which are a safety hazard for handling and storage, regulated for transportation or final disposal.
Located 3.5 miles north of I-85 on SC 187, our EM Lab has several state-of-art high resolution transmission electron microscopes (TEM), scanning electron microscopes (SEM) and a combined Focused Ion Beam (FIB)/SEM microscope. Our goal is to provide best microscopy and analytical services to our faculty and industrial collaborators. We frequently utilize Energy Dispersive X-ray Spectroscopy (EDS), Electron Backscatter Diffraction (EBSD), Wavelength dispersive spectroscopy (WDS), sample manipulation and surface modification capabilities installed on our electron microscopes. The multi-user facility attracts clients from _________________________________________________________________________________________________. The affordable cost, wide range of capabilities and minimum wait time make the facility very attractive to area researchers.
SEM stands for scanning electron microscope. The SEM is a microscope that uses electrons instead of light to form an image. Since their development in the early 1950's, scanning electron microscopes have developed new areas of study in the medical and physical science communities. The SEM has allowed researchers to examine a much bigger variety of specimens.
The scanning electron microscope has many advantages over traditional microscopes. The SEM has a large depth of field, which allows more of a specimen to be in focus at one time. The SEM also has much higher resolution, so closely spaced specimens can be magnified at much higher levels. Because the SEM uses electromagnets rather than lenses, the researcher has much more control in the degree of magnification. All of these advantages, as well as the actual strikingly clear images, make the scanning electron microscope one of the most useful instruments in research today.
The SEM is an instrument that produces a largely magnified image by using electrons instead of light to form an image. A beam of electrons is produced at the top of the microscope by an electron gun. The electron beam follows a vertical path through the microscope, which is held within a vacuum. The beam travels through electromagnetic fields and lenses, which focus the beam down toward the sample. Once the beam hits the sample, electrons and X-rays are ejected from the sample.
Because the SEM utilizes vacuum conditions and uses electrons to form an image, special preparations must be done to the sample. All water must be removed from the samples because the water would vaporize in the vacuum. All metals are conductive and require no preparation before being used. All non-metals need to be made conductive by covering the sample with a thin layer of conductive material. This is done by using a device called a "sputter coater."
The sputter coater uses an electric field and argon gas. The sample is placed in a small chamber that is at a vacuum. Argon gas and an electric field cause an electron to be removed from the argon, making the atoms positively charged. The argon ions then become attracted to a negatively charged gold foil. The argon ions knock gold atoms from the surface of the gold foil. These gold atoms fall and settle onto the surface of the sample producing a thin gold coating.
The radiation safety concerns are related to the electrons that are backscattered from the sample, as well as X-rays produced in the process. Most SEMs are extremely well shielded and do not produce exposure rates greater than background. However, scanning electron microscopes are radiation-generating devices and should be at least inventoried. The Indiana State Department of Health requires that the machines be registered with their office using State Form 16866, Radiation Machine Registration Application. It is also important that the integrity of the shielding is maintained, that all existing interlocks are functioning, and that workers are aware of radiation safety considerations.
Tumor necrosis factor (TNF) is a critical host resistance factor against tuberculosis. However, excess TNF produces susceptibility by increasing mitochondrial reactive oxygen species (mROS), which initiate a signaling cascade to cause pathogenic necrosis of mycobacterium-infected macrophages. In zebrafish, we identified the mechanism of TNF-induced mROS in tuberculosis. Excess TNF in mycobacterium-infected macrophages elevates mROS production by reverse electron transport (RET) through complex I. TNF-activated cellular glutamine uptake leads to an increased concentration of succinate, a Krebs cycle intermediate. Oxidation of this elevated succinate by complex II drives RET, thereby generating the mROS superoxide at complex I. The complex I inhibitor metformin, a widely used antidiabetic drug, prevents TNF-induced mROS and necrosis of 1__________________________-infected zebrafish and human macrophages; metformin may therefore be useful in tuberculosis therapy. 5376163bf9
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