• Grifagni D, Lenci E, De Santis A, Orsetti A, Barracchia CG, Tedesco F, Bellini Puglielli R, Lucarelli F, Lauriola A, Assfalg M, Cantini F, Calderone V, Guardavaccaro D, Trabocchi A, D’Onofrio M, Ciofi-Baffoni S. Development of a GC-376 based peptidomimetic PROTAC as a Degrader of 3-Chymotrypsin-like Protease of SARS-CoV-2.

ACS Medicinal Chemistry Letters, 2024. Online ahead of print.

• Lospinoso Severini L, Loricchio E, Navacci S, Basili I, Alfonsi R, Bernardi F, Moretti M, Conenna M, Cucinotta A, Coni S, Petroni M, De Smaele E, Giannini G, Maroder M, Canettieri G, Mastronuzzi A, Guardavaccaro D, Ayrault O, Infante P, Bufalieri F, and Di Marcotullio L. SALL4 is a CRL3REN/KCTD11 substrate that drives Sonic Hedgehog-dependent medulloblastoma.

Cell Death Differ 2023 Dec 7. Online ahead of print.

• Juliana Haydeé Enriqué Steinberg, Matteo Santucci, Fabiana Alejandra Rossi, Roberto Magliozzi, Laurensia Yuniati, Mario Rossi, Daniele Guardavaccaro, and Angela Lauriola. SCFbTrCP-mediated degradation of SHARP1 in triple-negative breast cancer.

Cell Death Dis Nov 8;14(11):726 2023.

• Felipe J. Renna, Juliana H. Enriqué Steinberg, Claudio D. Gonzalez, Maria Manifava, Mariana S. Tadic, Tamara Orquera, Carolina V. Vecino, Alejandro Ropolo, Daniele Guardavaccaro, Mario Rossi, Nicholas T. Ktistakis, Maria I. Vaccaro. Ubiquitination is a novel post-translational modification of VMP1 in autophagy of human tumor cells.

Int J Mol Sci Aug 19;24(16):12981, 2023.

• Dalle Carbonare L, Gomez Lira M, Minoia A, Bertacco J, Orsi S, Lauriola A, Li Vigni V, Gandini A, Antoniazzi F, Zipeto D, Mottes M, Bhandary L, Guardavaccaro D, Valenti MT. Expression of FBXW11 in normal and disease-associated osteogenic cells.

J Cell Mol Med 27(11):1580-1591, 2023.

• Liang D, Jiang L, Bhat SA, Jiang L, Missiroli S, Perrone M, Lauriola A, Adhikari R, Gudur A, Vasi Z, Ahearn I, Guardavaccaro D, Giorgi C, Philips M and Kuchay S. Palmitoylation and PDE6δ Regulate Membrane-compartment Specific Substrate Ubiquitylation and Degradation.

Cell Reports 31;42(1):111999, 2023.

• Lovison D, Alessi D, Allegri L, Baldan F, Ballico M, Damante G, Galasso M, Guardavaccaro D, Ruggieri S, Melchior A, Veclani D, Nardon C, Baratta W. Enantioselective Cytotoxicity of Chiral Diphosphine Ruthenium(II) Complexes Against Cancer Cells.

Chemistry 10;28(33):e202200200, 2022.

• Ishihara S, Sato T, Du G, Guardavaccaro D, Nakajima A, Sawai S, Kataoka T, Katagiri K. Phosphatidic acid-dependent localization and basal de-phosphorylation of RA-GEFs regulate lymphocyte trafficking.

BMC Biol 18(1):75, 2020

• Yuniati L*, Lauriola A*, Gerritsen M, Abreu S, Ni E, Tesoriero C, Onireti JO, Low, TY, Heck, AJ, Vettori A, Cardozo T, and Guardavaccaro D.Ubiquitylation of the ER-shaping protein Lunapark via the CRL3KLHL12 ubiquitin ligase complex. # Equal contribution.

Cell Reports 31(7):107664, 2020. *Equal contribution.

• Lauriola A, Martello A, Fantini S, Marverti G, Zanocco-Marani T, Davalli P, Guardavaccaro D, Mai S, Caporali A, and D’Arca D. Depletion of Trichoplein (TpMs) Causes Chromosome Mis-segregation, DNA Damage and Chromosome Instability in Cancer Cells.

Cancers 12(4):993, 2020.

• Spiombi E, Angrisani A, Fonte S, De Feudis G, Fabretti F, Cucchi D, Izzo M, Infante P, Miele E, Po A, Di Magno L, Magliozzi R, Guardavaccaro D, Maroder M, Canettieri G, Giannini G, Ferretti E, Gulino A, Di Marcotullio L, Moretti M, De Smaele E. KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.

Oncogenesis 8(11):64, 2019.

• Yuan R, Liu Q, Segeren HA, Yuniati L, Guardavaccaro D, Lebbink RJ, Westendorp B, de Bruin A. Cyclin F-dependent degradation of E2F7 is critical for DNA repair and G2-phase progression.

EMBO J 38(20):e101430, 2019 

• Bufalieri F, Infante P, Bernardi F, Caimano M, Romania P, Moretti M, Lospinoso Severini L, Talbot J, Melaiu O, Tanori M, Di Magno L, Bellavia D, Puget S, De Smaele E, Canettieri G, Guardavaccaro D, Busino L, Peschiaroli A, Pazzaglia S, Giannini G, Melino G, Locatelli F, Gulino A, Ayrault O, Fruci D, Capalbo C, and Di Marcotullio L. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.

Nature Commun 10(1):3304, 2019

• Mavrommati, I., Faedda, R., Galasso, G., Li, J., Burdova, K., Fisher, R., Kessler, B.M., Carrero, Z.I., Guardavaccaro, D., Pagano M., D’Angiolella V. βTrCP and Casein Kinase IIα mediated degradation of cyclin F controls timely mitotic progression.

Cell Reports 24(13):3404-3412, 2018

• Magliozzi, R.*, Carrero, Z.I.*, Low, T.Y., Yuniati, L., Valdes-Quezada. C., Kruiswijk, F., van Wijk, K., Heck, A.J., Jackson, C.L., Guardavaccaro, D. Inheritance of Golgi apparatus and cytokinesis are controlled by degradation of GBF1. 

    Cell Reports 23(11):3381-3391, 2018. *Equal contribution.

• Infante, P., Faedda, R., Bernardi, F., Bufalieri, F., Severini, L.L., Alfonsi, R., Mazzà, D., Siler, M., Coni, S., Po, A., Petroni, M., Ferretti, E., Mori, M., De Smaele, E., Canettieri, G., Capalbo, C., Maroder, M., Screpanti, I., Kool, M., Pfister, S., Guardavaccaro, D., Gulino, A., Di Marcotullio, L. Itch/βarrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signaling and medulloblastoma tumorigenesis. 

Nature Commun 9(1):976, 2018.

• D’Angiolella, V., Guardavaccaro, D. Two paths to let the replisome go.

Cell Death Differ 24(7):1140-1141, 2017.

• Tessadori, F., Noël, E.S., Rens, E.G., Magliozzi, R., Evers-van Gogh, I.J.A., Guardavaccaro, D., Merks, R.M.H., Bakkers, J. Nodal signalling range is regulated by proprotein convertase-mediated secretion. 

    Dev Cell 9;32(5):631-9, 2015.

• Low, T.Y., Peng, M., Magliozzi, R., Mohammed, S., Guardavaccaro, D., Heck, A.J. A systems-wide screen identifies substrates of the SCFβTrCPubiquitin ligase. 

    Science Signaling 7(356):rs8, 2014.

• Kim, J., D'Annibale, S., Magliozzi, R., Low, T.Y., Jansen, P., Shaltiel, I.A., Mohammed, S., Heck, A.J., Medema, R.H., Guardavaccaro, D. USP17- and SCFβTrCP-regulated degradation of DEC1 controls the DNA damage response.

    Mol Cell Biol. 34(22):4177-85, 2014. 

• Magliozzi, R., Kim, J., Low, T.Y., Heck, A.J., Guardavaccaro, D. Degradation of Tiam1 by casein kinase 1 and the SCFβTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling.

    J Biol Chem. 289(40):27400-9, 2014. 

• D'Annibale, S., Kim, J., Magliozzi, R., Low, T.Y., Mohammed, S., Heck, A.J., Guardavaccaro, D. Proteasome-dependent degradation of Transcription Factor AP4 (TFAP4) controls mitotic division.

    J Biol Chem 289(11):7730-7, 2014.

• Magliozzi, R., Low, T.W., Weijts, B.G.M.W., Cheng, T., Spanjaard, E., Mohammed, S., van Veen, A., Ovaa, H., de Rooij, J., Zwartkruis, F.J.T., Bos, J.L., de Bruin, A., Heck, A.J.R., Guardavaccaro, D. Control of epithelial cell migration and invasion by the IKKβ- and CK1α-mediated degradation of RAPGEF2.

    Dev Cell 27(5):574-85, 2013.

• Karim, R., Tummers, B., Meyers, C., Backendorf, C., Offringa, R., van Ommen, G.B., Melief, C.J.M., M. Boer, J, Guardavaccaro, D., van der Burg, S.H. Human papillomavirus (HPV) upregulates the cellular deubiquitinase UCHL1 to suppress the keratinocyte's innate immune response.

    PLoS Pathogens 9(5), May 2013.

• Low, T.Y., Magliozzi, R., Guardavaccaro, D., Heck, A.J.R. Unraveling the ubiquitin-regulated signaling networks by mass spectrometry-based proteomics

    Proteomics 13(3-4):526-3,7 2013.

• Kruiswijk, F., Yuniati, L., Magliozzi, R., Low, T.W., Lim, R., Bolder, R., Mohammed, S., Proud, C.G., Heck, A.J.R., Pagano, M., Guardavaccaro, D. Coupled activation-degradation of eEF2K regulates protein synthesis in response to genotoxic stress.

    Science Signaling 5(227):ra40, 2012.

    Comment in: Science Signaling 5(227):pe25, 2012

• Ping, Z., Lim, R., Bashir, T., Pagano, M., Guardavaccaro, D. APC/CCdh1 controls the proteasome-mediated degradation of E2F3 during cell cycle exit.

    Cell Cycle 11(10):1999-2005, 2012.

• Tee, J.M., Sartori da Silva, M., Rygiel, A., Muncan, V., Bink, R., van den Brink, G., van Tijn, P., Zivkovic, D., Kodach, L., Guardavaccaro, D., Diks, S., Peppelenbosch, M. Asb11 is a regulator of embryonic and adult regenerative myogenesis.

    Stem Cells Dev 21(17):3091-103, 2012.

• Guardavaccaro, D., Clevers, H. Wnt/β-Catenin and MAPK Signaling: Allies and Enemies in Different Battlefields.

    Science Signaling 5(219):pe155, 2012.

• de Lau, W., Barker, N., Low, T.Y., Koo, B.K., Li, V.S., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P.J., van de Wetering, M., Stange, D.E., van Es, J.E., Guardavaccaro, D., Schasfoort, R.B., Mohri, Y., Nishimori, K., Mohammed, S., Heck, A.J., Clevers, H. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling.

    Nature 476(7360):293-7, 2011.

    Comment in: Nature 476(7360), 2011.

• Sartori da Silva, M.A., Tee, J.M., Paridaen, J., Brouwers, A., Runtuwene, V., Zivkovic, D., Diks, S.H., Guardavaccaro, D., Peppelenbosch, M.P. Essential role for the d-Asb11 cul5 Box domain for proper notch signaling and neural cell fate decisions in vivo.

    PLoS One 5(11):e14023, 2010.

• Dehan, E., Bassermann, F., Guardavaccaro, D., Vasiliver-Shamis, G., Cohen, M.S., Lowes, K.N., Dustin, M., Huang, D.S., Taunton, J., Pagano, M. bTrCP- and Rsk1/2-mediated degradation of BimEL inhibits apoptosis.

    Mol Cell 33(1):109-16, 2009.

• Frescas, D., Guardavaccaro, D., Kuchay, S.M., Kato, H., Poleshko, A., Basrur, V., Elenitoba-Johnson, K.S., Katz, R.A., Pagano, M. KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state.

    Cell Cycle 7(22):3539-47, 2008.

    Comment in: Cell Cycle 7(22):3479-80, 2008. 

• Bassermann, F., Frescas, D., Guardavaccaro, D., Busino, L., Peschiaroli, A., Pagano, M. The Cdc14B-Cdh1-Plk1 axis in the control of the G2 DNA damage response checkpoint.

    Cell 134(2):256-67, 2008.

    Comment in: Cell 134(2):210-2, 2008.

• Zhao, X., Ik-Tsen Heng, J., Guardavaccaro, D., Jiang, R., Pagano, M., Guillemot, F., Iavarone, A., Lasorella, A. The HECT-domain ubiquitin ligase Ureb1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein.

    Nat Cell Biol 10(6):643-53, 2008.

• Michaelson, D., Abidi, W., Guardavaccaro, D., Zhou, M., Ahearn, I., Pagano, M., Philips, M.R. Rac1 accumulates in the nucleus during the G2 phase of the cell cycle and promotes cell division.

    J Cell Biol 181(3), 485-496, 2008.

• Guardavaccaro, D., Frescas, D., Dorrello, N.V., Peschiaroli, A., Multani, A.S., Cardozo, T., Lasorella, A., Iavarone, A., Chang, S., Hernando, E., Pagano, M. Control of chromosome stability by the βTRCP-REST-MAD2 axis.

Nature 452(7185) 365-70, 2008. 

    Comment in: Cancer Cell. 13(5):381-3, 2008 

    Comment in: Nature Reviews Cancer. 8(5):327, 2008.

• Frescas, D., Guardavaccaro, D., Bassermann, F., Koyama-Nasu, R., Pagano, M. The histone demethylase JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes. 

    Nature 450(7167) 309-13, 2007.

• Amador, V., Ge, S., Santamaria, P.G., Guardavaccaro, D., Pagano, M. APC/CCdc20 controls the ubiquitin-mediated degradation of p21 in prometaphase

    Mol Cell 27(3):462-73, 2007.

• Fielenbach, N., Guardavaccaro, D., Neubert, K., Chan, T., Li, D., Feng, Q., Hutter, H., Pagano M., Antebi A. DRE-1, an evolutionarily conserved F-box protein that regulates C. elegans developmental age.

    Dev Cell 12(3):443-55, 2007.

• Dorrello, N.V., Peschiaroli, A., Guardavaccaro, D., Colburn, N.H., Sherman, N.E., Pagano, M. SCFβTrCP- and S6K1-mediated degradation of PDCD4 promotes protein translation and cell growth.

    Science 314(5798):467-71, 2006.

    Comment in: Science 314(5798):428-9, 2006

• Peschiaroli, A., Dorrello, N.V., Guardavaccaro, D., Venere, M., Halazonetis, T., Sherman, N.E., Pagano, M. SCFβTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response.  

    Mol Cell 23(3):319-29, 2006.

    Comment in: Curr Biol 16(21):R932-4, 2006.

• Lasorella, A., Stegmüller, J. Guardavaccaro, D., Liu, G., Carro, M.S., Rothschild, G., de la Torre-Ubieta, L., Pagano, M., Bonni, A., Iavarone, A. Degradation of Id2 by the Anaphase Promoting Complex couples control of cell cycle exit and axonal growth.

    Nature 442(7101):471-4, 2006.

    Comment in: Nature 442(7101):365-6, 2006

• Ji, P., Goldin, L., Ren, H., Sun, D., Guardavaccaro, D., Pagano, M., Zhu, L. Skp2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27.

    J Biol Chem 281(33):24058-69, 2006.

• Guardavaccaro, D., Pagano M. Stabilizers and destabilizers controlling cell cycle oscillators.

    Mol Cell 22(1):1-4, 2006.

• Kudo, Y.*, Guardavaccaro, D.*, Gonzalez Santamaria, P., Koyama-Nasu, R., Latres, E. Bronson, R. Yamasaki, L. Pagano M.  Role of F-box protein βTrCP1 in mammary gland development and tumorigenesis. 

    Mol Cell Biol 24(18):8184-8194, 2004. *Equal contribution.

• Guardavaccaro, D., Pagano, M. Oncogenic aberrations of cullin-dependent ubiquitin ligases. 

    Oncogene  23(11):2037-49, 2004.

• Bashir, T., Dorrello, N.V., Amador, V., Guardavaccaro, D., Pagano, M. Control of the SCFSkp2-Cks1 ubiquitin ligase by the APC/CCdh1 ubiquitin ligase.  

    Nature 428(6979):190-3, 2004.

    Comment in: Nature Reviews Molecular Cell Biology 5, 339 2004

• Busino, L., Donzelli, M., Chiesa, M., Guardavaccaro, D., Ganoth, D., Dorrello, N.V., Hershko, A., Pagano M., Draetta G. Ubiquitin-mediated degradation of Cdc25 by βTrCP during S phase and in response to DNA damage. 

    Nature 426(6962):87-9, 2003.

• Guardavaccaro, D., Kudo, Y. Boulaire, J., Barchi, M., Busino, L., Donzelli, M., Yamasaki, L., Pagano, M. Control of meiotic and mitotic progression by the F-box protein βTrCP1 in vivo. 

    Dev Cell 4: 799–812, 2003.

    Comment in: Mol Cell 11(6):1420-1, 2003.

• Corrente, G., Guardavaccaro, D., Tirone, F. PC3 potentiates NGF-induced differentiation and protects neurons from apoptosis. 

    Neuroreport 13(4):417-22, 2002.

• Guardavaccaro, D., Corrente, G., Covone, F., Micheli, L., D'Agnano, I., Starace, G., Caruso M., Tirone F. Arrest of G(1)-S progression by the p53-inducible gene PC3 is Rb dependent and relies on the inhibition of cyclin D1 transcription. 

    Mol Cell Biol 20: 1797-815, 2000.

• Cenciarelli, C., Chiaur, D. S., Guardavaccaro, D., Parks, W., Vidal, M., Pagano M. Identification of a family of human F-box proteins.  

    Curr Biol 9: 1177-1179, 1999.

• Buanne, P., Incerti, B., Guardavaccaro, D., Avvantaggiato, V., Simeone A., Tirone F. Cloning of the human Interferon-Related Developmental Regulator (IFRD1) gene coding for the PC4 protein, member of a novel family of developmentally regulated genes.  

    Genomics 51: 233-42, 1998.

• Montagnoli, A., Guardavaccaro, D., Starace G., Tirone F. Overexpression of the PC3 immediate early gene is associated to inhibition of cell proliferation. 

    Cell Growth Differ 7: 1327-1336, 1996.

• Guardavaccaro, D., Ciotti, M.T., Schafer, B.W., Montagnoli A., Tirone F. Inhibition of differentiation in myoblasts deprived of the interferon-related protein PC4. 

    Cell Growth Differ 6: 159-169, 1995.

• Guardavaccaro, D., Montagnoli, A., Ciotti, M.T., Lotti, L., Di Lazzaro, C., Torrisi, M.R., Gatti A., Tirone F. Nerve Growth Factor regulates the subcellular localization of the NGF inducible protein PC4 in PC12 cells. 

    J Neurosci Res 37: 660-674, 1994.