Lauriola A*, Enriqué Steinberg JH*, Sarubo M, Maspero E, Rossi FA, Mouri Y, Pedretti M, Hajisadeghian M, Taibi V, Vettori A, Vitulo N, Assfalg M, D’Onofrio M, Rossi M, Yasue A, Astegno A, Polo S, Santi S, Kudo Y*, and Guardavaccaro D*. The E3 ligase RNF32 controls the IkB kinase complex and NF-kB signaling in intestinal stem cells.
Tsunematsu T, Mouri Y, Shao W, Arakaki R, Jan G. Ruppert JG, Murano K, Ishimaru N, Guardavaccaro D, Pagano M, and Kudo Y.
Sustained chromosomal passenger complex activity preserves the pluripotency of human embryonic carcinoma cells.
Science Signaling, 2025 Feb 18;18(874):eadg4626
Dalle Carbonare L, Minoia A, Gandini A, Piritore FC, Patuzzo C, Ceretti L, Vareschi A, Aparo A, Cominacini M, Malerba G, Romanelli MG, Pessoa J, Guardavaccaro D, Antoniazzi F, Valenti MT. Unraveling RUNX2 mutation in a cleidocranial dysplasia patient: Molecular insights into osteogenesis and proteostasis.
Genes & Diseases, 2024 Nov 6;12(4):101449
Dalle Carbonare L, Minoia A, Vareschi A, Piritore FC, Zouari S, Gandini A, Meneghel M, Elia R, Lorenzi P, Antoniazzi F, Pessoa J, Zipeto D, Romanelli MG, Guardavaccaro D, Valenti MT. Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression.
Grifagni D, Lenci E, De Santis A, Orsetti A, Barracchia CG, Tedesco F, Bellini Puglielli R, Lucarelli F, Lauriola A, Assfalg M, Cantini F, Calderone V, Guardavaccaro D, Trabocchi A, D’Onofrio M, Ciofi-Baffoni S. Development of a GC-376 based peptidomimetic PROTAC as a Degrader of 3-Chymotrypsin-like Protease of SARS-CoV-2.
ACS Medicinal Chemistry Letters, 2024 Jan 10;15(2):250-257
Lospinoso Severini L, Loricchio E, Navacci S, Basili I, Alfonsi R, Bernardi F, Moretti M, Conenna M, Cucinotta A, Coni S, Petroni M, De Smaele E, Giannini G, Maroder M, Canettieri G, Mastronuzzi A, Guardavaccaro D, Ayrault O, Infante P, Bufalieri F, and Di Marcotullio L. SALL4 is a CRL3REN/KCTD11 substrate that drives Sonic Hedgehog-dependent medulloblastoma.
Cell Death Differ 2024 Feb;31(2):170-187
Juliana Haydeé Enriqué Steinberg, Matteo Santucci, Fabiana Alejandra Rossi, Roberto Magliozzi, Laurensia Yuniati, Mario Rossi, Daniele Guardavaccaro, and Angela Lauriola. SCFbTrCP-mediated degradation of SHARP1 in triple-negative breast cancer.
Cell Death Dis Nov 8;14(11):726 2023
Felipe J. Renna, Juliana H. Enriqué Steinberg, Claudio D. Gonzalez, Maria Manifava, Mariana S. Tadic, Tamara Orquera, Carolina V. Vecino, Alejandro Ropolo, Daniele Guardavaccaro, Mario Rossi, Nicholas T. Ktistakis, Maria I. Vaccaro. Ubiquitination is a novel post-translational modification of VMP1 in autophagy of human tumor cells.
Int J Mol Sci Aug 19;24(16):12981, 2023
Dalle Carbonare L, Gomez Lira M, Minoia A, Bertacco J, Orsi S, Lauriola A, Li Vigni V, Gandini A, Antoniazzi F, Zipeto D, Mottes M, Bhandary L, Guardavaccaro D, Valenti MT. Expression of FBXW11 in normal and disease-associated osteogenic cells.
J Cell Mol Med 27(11):1580-1591, 2023
Liang D, Jiang L, Bhat SA, Jiang L, Missiroli S, Perrone M, Lauriola A, Adhikari R, Gudur A, Vasi Z, Ahearn I, Guardavaccaro D, Giorgi C, Philips M and Kuchay S. Palmitoylation and PDE6δ Regulate Membrane-compartment Specific Substrate Ubiquitylation and Degradation.
Cell Reports 31;42(1):111999, 2023
Lovison D, Alessi D, Allegri L, Baldan F, Ballico M, Damante G, Galasso M, Guardavaccaro D, Ruggieri S, Melchior A, Veclani D, Nardon C, Baratta W. Enantioselective Cytotoxicity of Chiral Diphosphine Ruthenium(II) Complexes Against Cancer Cells.
Chemistry 10;28(33):e202200200, 2022
Ishihara S, Sato T, Du G, Guardavaccaro D, Nakajima A, Sawai S, Kataoka T, Katagiri K. Phosphatidic acid-dependent localization and basal dephosphorylation of RA-GEFs regulate lymphocyte trafficking.
Yuniati L*, Lauriola A*, Gerritsen M, Abreu S, Ni E, Tesoriero C, Onireti JO, Low, TY, Heck, AJ, Vettori A, Cardozo T, and Guardavaccaro D. Ubiquitylation of the ER-shaping protein Lunapark via the CRL3KLHL12 ubiquitin ligase complex. # Equal contribution.
Cell Reports 31(7):107664, 2020 *Equal contribution.
Lauriola A, Martello A, Fantini S, Marverti G, Zanocco-Marani T, Davalli P, Guardavaccaro D, Mai S, Caporali A, and D’Arca D. Depletion of Trichoplein (TpMs) Causes Chromosome Mis-segregation, DNA Damage and Chromosome Instability in Cancer Cells.
Spiombi E, Angrisani A, Fonte S, De Feudis G, Fabretti F, Cucchi D, Izzo M, Infante P, Miele E, Po A, Di Magno L, Magliozzi R, Guardavaccaro D, Maroder M, Canettieri G, Giannini G, Ferretti E, Gulino A, Di Marcotullio L, Moretti M, De Smaele E. KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.
Yuan R, Liu Q, Segeren HA, Yuniati L, Guardavaccaro D, Lebbink RJ, Westendorp B, de Bruin A. Cyclin F-dependent degradation of E2F7 is critical for DNA repair and G2-phase progression.
Bufalieri F, Infante P, Bernardi F, Caimano M, Romania P, Moretti M, Lospinoso Severini L, Talbot J, Melaiu O, Tanori M, Di Magno L, Bellavia D, Puget S, De Smaele E, Canettieri G, Guardavaccaro D, Busino L, Peschiaroli A, Pazzaglia S, Giannini G, Melino G, Locatelli F, Gulino A, Ayrault O, Fruci D, Capalbo C, and Di Marcotullio L. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.
Nature Commun 10(1):3304, 2019
Mavrommati, I., Faedda, R., Galasso, G., Li, J., Burdova, K., Fisher, R., Kessler, B.M., Carrero, Z.I., Guardavaccaro, D., Pagano M., D’Angiolella V. βTrCP and Casein Kinase IIα mediated degradation of cyclin F controls timely mitotic progression.
Cell Reports 24(13):3404-3412, 2018
Magliozzi, R.*, Carrero, Z.I.*, Low, T.Y., Yuniati, L., Valdes-Quezada. C., Kruiswijk, F., van Wijk, K., Heck, A.J., Jackson, C.L., Guardavaccaro, D. Inheritance of Golgi apparatus and cytokinesis are controlled by degradation of GBF1.
Cell Reports 23(11):3381-3391, 2018 *Equal contribution.
Infante, P., Faedda, R., Bernardi, F., Bufalieri, F., Severini, L.L., Alfonsi, R., Mazzà, D., Siler, M., Coni, S., Po, A., Petroni, M., Ferretti, E., Mori, M., De Smaele, E., Canettieri, G., Capalbo, C., Maroder, M., Screpanti, I., Kool, M., Pfister, S., Guardavaccaro, D., Gulino, A., Di Marcotullio, L. Itch/βarrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signaling and medulloblastoma tumorigenesis.
D’Angiolella, V., Guardavaccaro, D. Two paths to let the replisome go.
Cell Death Differ 24(7):1140-1141, 2017
Tessadori, F., Noël, E.S., Rens, E.G., Magliozzi, R., Evers-van Gogh, I.J.A., Guardavaccaro, D., Merks, R.M.H., Bakkers, J. Nodal signalling range is regulated by proprotein convertase-mediated secretion.
Low, T.Y., Peng, M., Magliozzi, R., Mohammed, S., Guardavaccaro, D., Heck, A.J. A systems-wide screen identifies substrates of the SCFβTrCPubiquitin ligase.
Science Signaling 7(356):rs8, 2014
Kim, J., D'Annibale, S., Magliozzi, R., Low, T.Y., Jansen, P., Shaltiel, I.A., Mohammed, S., Heck, A.J., Medema, R.H., Guardavaccaro, D. USP17- and SCFβTrCP-regulated degradation of DEC1 controls the DNA damage response.
Mol Cell Biol. 34(22):4177-85, 2014
Magliozzi, R., Kim, J., Low, T.Y., Heck, A.J., Guardavaccaro, D. Degradation of Tiam1 by casein kinase 1 and the SCFβTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling.
J Biol Chem. 289(40):27400-9, 2014
D'Annibale, S., Kim, J., Magliozzi, R., Low, T.Y., Mohammed, S., Heck, A.J., Guardavaccaro, D. Proteasome-dependent degradation of Transcription Factor AP4 (TFAP4) controls mitotic division.
J Biol Chem 289(11):7730-7, 2014
Magliozzi, R., Low, T.W., Weijts, B.G.M.W., Cheng, T., Spanjaard, E., Mohammed, S., van Veen, A., Ovaa, H., de Rooij, J., Zwartkruis, F.J.T., Bos, J.L., de Bruin, A., Heck, A.J.R., Guardavaccaro, D. Control of epithelial cell migration and invasion by the IKKβ- and CK1α-mediated degradation of RAPGEF2.
Karim, R., Tummers, B., Meyers, C., Backendorf, C., Offringa, R., van Ommen, G.B., Melief, C.J.M., M. Boer, J, Guardavaccaro, D., van der Burg, S.H. Human papillomavirus (HPV) upregulates the cellular deubiquitinase UCHL1 to suppress the keratinocyte's innate immune response.
Low, T.Y., Magliozzi, R., Guardavaccaro, D., Heck, A.J.R. Unraveling the ubiquitin-regulated signaling networks by mass spectrometry-based proteomics
Proteomics 13(3-4):526-3,7 2013
Kruiswijk, F., Yuniati, L., Magliozzi, R., Low, T.W., Lim, R., Bolder, R., Mohammed, S., Proud, C.G., Heck, A.J.R., Pagano, M., Guardavaccaro, D. Coupled activation-degradation of eEF2K regulates protein synthesis in response to genotoxic stress.
Science Signaling 5(227):ra40, 2012
Comment in: Science Signaling 5(227):pe25, 2012
Ping, Z., Lim, R., Bashir, T., Pagano, M., Guardavaccaro, D. APC/CCdh1 controls the proteasome-mediated degradation of E2F3 during cell cycle exit.
Cell Cycle 11(10):1999-2005, 2012
Tee, J.M., Sartori da Silva, M., Rygiel, A., Muncan, V., Bink, R., van den Brink, G., van Tijn, P., Zivkovic, D., Kodach, L., Guardavaccaro, D., Diks, S., Peppelenbosch, M. Asb11 is a regulator of embryonic and adult regenerative myogenesis.
Stem Cells Dev 21(17):3091-103, 2012
Guardavaccaro, D., Clevers, H. Wnt/β-Catenin and MAPK Signaling: Allies and Enemies in Different Battlefields.
Science Signaling 5(219):pe155, 2012
de Lau, W., Barker, N., Low, T.Y., Koo, B.K., Li, V.S., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P.J., van de Wetering, M., Stange, D.E., van Es, J.E., Guardavaccaro, D., Schasfoort, R.B., Mohri, Y., Nishimori, K., Mohammed, S., Heck, A.J., Clevers, H. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling.
Comment in: Nature 476(7360), 2011
Sartori da Silva, M.A., Tee, J.M., Paridaen, J., Brouwers, A., Runtuwene, V., Zivkovic, D., Diks, S.H., Guardavaccaro, D., Peppelenbosch, M.P. Essential role for the d-Asb11 cul5 Box domain for proper notch signaling and neural cell fate decisions in vivo.
Dehan, E., Bassermann, F., Guardavaccaro, D., Vasiliver-Shamis, G., Cohen, M.S., Lowes, K.N., Dustin, M., Huang, D.S., Taunton, J., Pagano, M. bTrCP- and Rsk1/2-mediated degradation of BimEL inhibits apoptosis.
Frescas, D., Guardavaccaro, D., Kuchay, S.M., Kato, H., Poleshko, A., Basrur, V., Elenitoba-Johnson, K.S., Katz, R.A., Pagano, M. KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state.
Cell Cycle 7(22):3539-47, 2008
Comment in: Cell Cycle 7(22):3479-80, 2008
Bassermann, F., Frescas, D., Guardavaccaro, D., Busino, L., Peschiaroli, A., Pagano, M. The Cdc14B-Cdh1-Plk1 axis in the control of the G2 DNA damage response checkpoint.
Comment in: Cell 134(2):210-2, 2008
Zhao, X., Ik-Tsen Heng, J., Guardavaccaro, D., Jiang, R., Pagano, M., Guillemot, F., Iavarone, A., Lasorella, A. The HECT-domain ubiquitin ligase Ureb1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein.
Nat Cell Biol 10(6):643-53, 2008
Michaelson, D., Abidi, W., Guardavaccaro, D., Zhou, M., Ahearn, I., Pagano, M., Philips, M.R. Rac1 accumulates in the nucleus during the G2 phase of the cell cycle and promotes cell division.
J Cell Biol 181(3), 485-496, 2008
Guardavaccaro, D., Frescas, D., Dorrello, N.V., Peschiaroli, A., Multani, A.S., Cardozo, T., Lasorella, A., Iavarone, A., Chang, S., Hernando, E., Pagano, M. Control of chromosome stability by the βTRCP-REST-MAD2 axis.
Comment in: Cancer Cell. 13(5):381-3, 2008
Comment in: Nature Reviews Cancer. 8(5):327, 2008
Frescas, D., Guardavaccaro, D., Bassermann, F., Koyama-Nasu, R., Pagano, M. The histone demethylase JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes.
Amador, V., Ge, S., Santamaria, P.G., Guardavaccaro, D., Pagano, M. APC/CCdc20 controls the ubiquitin-mediated degradation of p21 in prometaphase
Fielenbach, N., Guardavaccaro, D., Neubert, K., Chan, T., Li, D., Feng, Q., Hutter, H., Pagano M., Antebi A. DRE-1, an evolutionarily conserved F-box protein that regulates C. elegans developmental age.
Dorrello, N.V., Peschiaroli, A., Guardavaccaro, D., Colburn, N.H., Sherman, N.E., Pagano, M. SCFβTrCP- and S6K1-mediated degradation of PDCD4 promotes protein translation and cell growth.
Science 314(5798):467-71, 2006
Comment in: Science 314(5798):428-9, 2006
Peschiaroli, A., Dorrello, N.V., Guardavaccaro, D., Venere, M., Halazonetis, T., Sherman, N.E., Pagano, M. SCFβTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response.
Comment in: Curr Biol 16(21):R932-4, 2006
Lasorella, A., Stegmüller, J. Guardavaccaro, D., Liu, G., Carro, M.S., Rothschild, G., de la Torre-Ubieta, L., Pagano, M., Bonni, A., Iavarone, A. Degradation of Id2 by the Anaphase Promoting Complex couples control of cell cycle exit and axonal growth.
Comment in: Nature 442(7101):365-6, 2006
Ji, P., Goldin, L., Ren, H., Sun, D., Guardavaccaro, D., Pagano, M., Zhu, L. Skp2 contains a novel cyclin A binding domain that directly protects cyclin A from inhibition by p27.
J Biol Chem 281(33):24058-69, 2006
Guardavaccaro, D., Pagano M. Stabilizers and destabilizers controlling cell cycle oscillators.
Kudo, Y.*, Guardavaccaro, D.*, Gonzalez Santamaria, P., Koyama-Nasu, R., Latres, E. Bronson, R. Yamasaki, L. Pagano M. Role of F-box protein βTrCP1 in mammary gland development and tumorigenesis.
Mol Cell Biol 24(18):8184-8194, 2004. *Equal contribution.
Guardavaccaro, D., Pagano, M. Oncogenic aberrations of cullin-dependent ubiquitin ligases.
Bashir, T., Dorrello, N.V., Amador, V., Guardavaccaro, D., Pagano, M. Control of the SCFSkp2-Cks1 ubiquitin ligase by the APC/CCdh1 ubiquitin ligase.
Comment in: Nature Reviews Molecular Cell Biology 5, 339 2004
Busino, L., Donzelli, M., Chiesa, M., Guardavaccaro, D., Ganoth, D., Dorrello, N.V., Hershko, A., Pagano M., Draetta G. Ubiquitin-mediated degradation of Cdc25 by βTrCP during S phase and in response to DNA damage.
Guardavaccaro, D., Kudo, Y. Boulaire, J., Barchi, M., Busino, L., Donzelli, M., Yamasaki, L., Pagano, M. Control of meiotic and mitotic progression by the F-box protein βTrCP1 in vivo.
Comment in: Mol Cell 11(6):1420-1, 2003
Corrente, G., Guardavaccaro, D., Tirone, F. PC3 potentiates NGF-induced differentiation and protects neurons from apoptosis.
Neuroreport 13(4):417-22, 2002
Guardavaccaro, D., Corrente, G., Covone, F., Micheli, L., D'Agnano, I., Starace, G., Caruso M., Tirone F. Arrest of G(1)-S progression by the p53-inducible gene PC3 is Rb dependent and relies on the inhibition of cyclin D1 transcription.
Mol Cell Biol 20: 1797-815, 2000
Cenciarelli, C., Chiaur, D. S., Guardavaccaro, D., Parks, W., Vidal, M., Pagano M. Identification of a family of human F-box proteins.
Buanne, P., Incerti, B., Guardavaccaro, D., Avvantaggiato, V., Simeone A., Tirone F. Cloning of the human Interferon-Related Developmental Regulator (IFRD1) gene coding for the PC4 protein, member of a novel family of developmentally regulated genes.
Montagnoli, A., Guardavaccaro, D., Starace G., Tirone F. Overexpression of the PC3 immediate early gene is associated to inhibition of cell proliferation.
Cell Growth Differ 7: 1327-1336, 1996
Guardavaccaro, D., Ciotti, M.T., Schafer, B.W., Montagnoli A., Tirone F. Inhibition of differentiation in myoblasts deprived of the interferon-related protein PC4.
Cell Growth Differ 6: 159-169, 1995
Guardavaccaro, D., Montagnoli, A., Ciotti, M.T., Lotti, L., Di Lazzaro, C., Torrisi, M.R., Gatti A., Tirone F. Nerve Growth Factor regulates the subcellular localization of the NGF inducible protein PC4 in PC12 cells.
J Neurosci Res 37: 660-674, 1994