NK Bairwa Lab
School of Biotechnology
Shri Mata Vaishno Devi University
Narendra K Bairwa, PhD
Assistant Professor and Ramalingaswami Fellow
School of Biotechnology,
Shri Mata Vaishno Devi University,
Katra, Reasi- Jammu & Kashmir- 182320 India
Research
The broad basic research focus of lab is to investigate the novel synthetic binary interactions among genes that are involved in conserved biological processes from yeast to humans using S. cerevisiae as a model organism for uncovering novel drug targets. We study gene-gene, protein-protein interactions, and disruption of pathways in understanding the mechanisms of synthetic lethality, genome stability regulation and stress response.
For the applied aspect we are working on synthetic engineering of the probiotic yeast S. boulardii using CRISPER/Cas9 approach for human therapeutics. The lab focus is also on the isolation, characterization, and exploration of wild yeast from the local environment for biotechnological applications.
Latest News
Researchers from Shri Mata Vaishno Devi University (SMVDU), Katra, Develops a Simple and Rapid Method to Study Apoptosis Induced by Chemical Treatment using Yeast as a model organism
Researchers from Shri Mata Vaishno Devi University, Katra led by Dr. Narendra K Bairwa, Assistant Professor, Genome Stability Regulation Lab, School of Biotechnology, SMVDU, recipient of the prestigious Ramalingaswami re-entry fellowship, develops a simple and rapid method to study apoptosis induced by chemical treatment using Yeast (Saccharomyces cerevisiae) as a model organism. The research method is published in the Current Protocols, 7th September 2022 issue and can be accessed using the following URL (https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.525).
Apoptosis is the conserved biological process that regulates growth, differentiation, and development in organisms including humans. The apoptosis process is implicated in various disease processes such as cancer, cardiovascular and aging. The current methods of apoptosis detection require specialized types of equipment, reagents, and skilled personnel that limit the study and assessment of apoptosis. The methods developed by Dr. NK Bairwa and his team can be utilized for the assessment of the toxicity of the chemicals by studying the features of apoptosis rapidly and accurately. The method can also be used for teaching the impact of chemicals derived from plants or industries on cells to a large number of students enrolled in schools, colleges, and universities. The authors, Narendra K Bairwa, Ms. Heena Shoket, Ms. Monika Pandita, and Ms. Meenu Sharma contributed to the published study titled “A Simple Assay for the Detection of Late‐Stage Apoptosis Features in Saccharomyces cerevisiae"
Research Scholar, Ms. Meenu Sharma received her Ph.D. degree certificate in the 8th convocation held on 29th July 2022 .
Research Scholars
Ms. Meenu Sharma
Ms. Monika Pandita
Ms. Heena Shoket
Publications:
Bairwa NK, Shoket H, Pandita M, Sharma M .A Simple Assay for the Detection of Late-Stage Apoptosis Features in Saccharomyces cerevisiae. Curr Protoc. 2022 Sep;2(9):e525. doi: 10.1002/cpz1.525.
Sharma M, Pandita M, Shoket H,Bairwa NK. F-box motif encoding genes as targets for the development of stress-tolerant traits in Saccharomyces cerevisiae. Indian Journal of Experimental Biology (IJEB) 2022 Sept. 60 (09) 667-671
Shoket H, Parvez S, Sharma M, Pandita M, Sharma V, Kumar P, Bairwa NK. Deletion of autophagy related, ATG1 and F-box motif encoding YDR131C, together, lead to synthetic growth defects and flocculation behavior in Saccharomyces cerevisiae. J Biochem Mol Toxicol. 2022 Apr 6:e23064. doi:10.1002/jbt.23064. Epub ahead of print. PMID: 35385166
Sharma M, Verma V, Bairwa NK. Genetic interaction between RLM1 and F-box motif encoding gene SAF1 contributes to stress response in Saccharomyces cerevisiae. Genes Environ. 2021 Oct 9;43(1):45. doi: 10.1186/s41021-021-00218-x. PMID: 34627408; PMCID: PMC8501602.
Shoket H, Pandita M, Sharma M, Kumar R, Rakwal A, Wazir S, Verma V, Salunke DB, Bairwa NK. Genetic interaction between F-box motif encoding YDR131C and retrograde signaling-related RTG1 regulates the stress response and apoptosis in Saccharomyces cerevisiae. J Biochem Mol Toxicol. 2021 Oct;35(10):e22864. doi: 10.1002/jbt.22864. Epub 2021 Jul 26. PMID: 34309121
Pandita M, Shoket H, Rakewal A, Wazir S, Kumar P, Kumar R, Bairwa NK. Genetic interaction between glyoxylate pathway regulator UCC1 and La-motif-encoding SRO9 regulates stress response and growth rate improvement in Saccharomyces cerevisiae. J Biochem Mol Toxicol. 2021 Jul;35(7):e22781. doi: 10.1002/jbt.22781. Epub 2021 Apr 2. PMID: 33797855.
The absence of F-box motif Encoding Gene SAF1 and Chromatin Associated factor CTF8 together contributes to MMS Resistant and HU Sensitive phenotype in S. cerevisiae
Meenu Sharma, V. Verma, Narendra K Bairwa
bioRxiv 656363; doi: https://doi.org/10.1101/656363
Loss of F-box Motif Encoding Gene SAF1 and RRM3 Together Leads to Synthetic Growth Defect and Sensitivity to HU, MMS in S.cerevisiae
Meenu Sharma, V. Verma, Narendra K Bairwa
bioRxiv 636902; doi: https://doi.org/10.1101/636902
Absence of Replication fork associated factor CTF4 and F-box motif Encoding Gene SAF1 leads to reduction in Cell Size and Stress Tolerance Phenotype in S. cerevisiae
Meenu Sharma, Samar Singh, V. Verma, Narendra K Bairwa
bioRxiv 664185; doi: https://doi.org/10.1101/664185
Deletion of Autophagy gene ATG1 and Glyoxylate cycle Regulator encoding gene UCC1 together leads to Synthetic Growth Defects and Sensitivity to Genotoxic agents in Saccharomyces cerevisiae
Monika Pandita, Vishali Sharma, Meenu Sharma, Heena Shoket, Sadia Parvez, Prabhat Kumar, Narendra K Bairwa
bioRxiv 2020.03.03.974568; doi: https://doi.org/10.1101/2020.03.03.974568
An Assay for Apoptosis detection based on Quantification of Multi nuclei feature and Nucleus to Cytoplasm ratio in S. cerevisiae cells treated with Acetic Acid and Hydrogen peroxide
Narendra K Bairwa, Heena Shoket, Monika Pandita, Meenu Sharma
bioRxiv 2020.03.11.987024; doi: https://doi.org/10.1101/2020.03.11.987024
Other publications :
Nucleotide Sequence Database
Trainee Students
Ms. Adrija Mahajan (2022)
Student of B.Sc. Biotechnology at Amity University
Ms. Samiksha Sharma (2022)
Student of M.Sc. Biotechnology , SMVDU
Ms. Vaishnavi Verma (2022)
Student of 10th Grade at DPS, Katra
Ms. Ruhi Singh (2021)
Student of M.Sc. Biotechnology, SMVDU
Ms. Sadia Pervez (2020)
Student of M.Sc. Biotechnology, SMVDU
Ms. Neha Sharma (2021)
Student of M.Sc. Biotechnology, SMVDU
Photo Gallery
ICBYFF-2019
School of Life Sciences, University of Hyderabad
ICBYFF-2019 : Poster Presentation
ICBYFF-2019
GSR LAB
GSR Lab Members
National Symposium on Recent Trends in Biotechnology & Drug Discovery - 2017,
School of Biotechnology, SMVDU , Katra
Young Investigators Meeting -2015 Gulmerg
FASEB SUMMER RESEARCH CONFERENCES-2010 Carefree, Arizona
Outdoor activity
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Tea time
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Join us : Vacancy Available
We look forward meeting enthusiastic students (School, Colleges or any Curious mind) to explore the unexplored microscopic world of the region for internship or training.
Contact us
School of Biotechnology
Shri Mata Vaishno Devi University
Katra, Reasi- 182320
Jammu & Kashmir, India
Outreach Program (Public Engagement)
Funding
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GSA