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From the 18 leads identified by HTPA, the 5 antigens detailed above were taken forward for further analysis by R&D assay on scaled up R&D protein batches (highlighted in bold, italic font in Table 1). The optimized cut-off values determined using sample cohort 2 were validated by applying them to R&D assay results from cohort 1. The new cut-offs derived for the eCDT-Lung R&D assay panel were also applied to the commercial antigens. The cohort 1 R&D assay sensitivity and specificity values for each individual antigen are given in Table 2. It can be seen that the eCDT-Lung R&D test alone gave a panel sensitivity and specificity of 36% and 93% respectively when tested on this patient cohort. The confirmed leads alone gave sensitivity and specificity values ranging from 5 to 9% and 98 to 100% respectively. The sensitivity values for eCDT-Lung R&D panel in combination with each of the confirmed leads individually is highlighted in italic font and it can be seen that addition of each of the leads led to an improvement in sensitivity of the panel but did not always result in a decrease in specificity. Table 2 shows data for every combination of 2 and 3 confirmed leads with the eCDT-Lung R&D panel. The best combination of three confirmed leads with the panel was with either Lmyc2 and cytokeratin 20 or alpha-enolase and cytokeratin 20 which both led to sensitivity of 46% and specificity of 92%.

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Outside Woolwich is the lofty Shooters Hill,commanding a fine view of the Thames valley andLondon, that was in olden times a noted haunt ofhighwaymen, a fact to which it is supposed to oweits name. It is often alluded to by old chroniclers,notably by Phillpott, who declares that it was socalled for the 'thievery there practised wheretravellers in elder times were so much infested withdepredations and bloody mischief, that order wastaken in the 6th year of Richard II. for theenlarging the highway'; but the evil was not remedied,for as late as 1682 Oldham wrote that 'Padders came{90}from Shooters Hill in flocks.' In Hall's Chroniclethere is a noteworthy description of a meeting onShooters Hill between Henry VIII. and his queenand Robin Hood, which deserves quotation at length:'And as they passed by the way,' he says, 'theyespied a company of tall yeomen, clothed all in grenewith grene whodes and bowes and arrowes to thenumber of ii C. Then one of them, which calledhimselfe Robyn Hood, came to the kyng desyringhim to se his men shoote, and the kyng was content.Then he whistled, and al the ii C archers shot andlosed at once, and then he whisteled agayne and theylikewise shot agayne, their arrows whisteled by crafteof the head so that the noyes was strange and greatand much pleased the kynge and quene and all thecompany.' So delighted, indeed, was Henry with theprowess displayed, that when the bold Robin 'desyredthem to come into the grene wood and see how theoutlaws lyve,' they readily consented. 'Then,' addsthe chronicler, 'the hornes blew till they came to thewood under Shoters Hil, and there was an arbormade of boughs, with a hal and a great chambervery well made and covered with floures and sweteherbes, which the kyng much praysed.' Encouragedby this success, the outlaw chief made a yet bolderventure, for though he must have known that he wasrisking the lives of all his merry men as well as hisown, he said to the king, 'Outlawes brekefastes isvenyson, and therefore you must be content withsuch fare as we use.' Even this bold confession ofguilt, however, did not rouse the ire of the usuallyhasty monarch; he and his queen, says Hall, 'sate{91}doune and were served with venyson and wyne byRobin Hood and his men to their contentacion.'

Many significant stories are told of the doings ofthe maiden queen at Greenwich; how, for instance,she caused a dishonest purveyor of poultry to behanged on the complaint of a farmer who boldlyintercepted her on one of her progresses, crying in aloud voice, in spite of all the efforts of the attendantsto silence him, 'Which is the Queen? Which is theQueen?' Elizabeth herself replied to him, listenedto all he had to say, and granted his request withoutmore ado, although he dared to assume that she had{107}devoured the hens and ducks seized by her servant,declaring that she could eat more than his owndaughter, who was blessed with a very goodappetite.

The parish church of Chislehurst, though practicallymodern, was built on the lines of its sixteenth-centurypredecessor, and with its lofty spire presentsa picturesque appearance. It contains thealtar tomb of the Walsingham family and severalother noteworthy memorials, including a fifteenth-centurybrass in memory of Alan Porter, and amonument to William Selwyn, designed by Chantrey.The font is said to be of great antiquity, and maypossibly have been in use in Saxon times, and in{128}the churchyard are some interesting old tombs,including that in which rest the remains of Mr. andMrs. Bonar, who were murdered by their servant in1813.

Ewell, the name of which is a corruption of theSaxon twelle, signifying the village on the well,so called because it is close to the springs formingthe source of a stream known as the Hogsmill, thatjoins the Thames at Kingston, was but a shorttime ago a secluded village, but is now rapidlygrowing into a popular suburb. Unfortunately itscharacteristic old market-hall has been pulleddown, and of the ancient church the tower aloneremains, but in its modern successor are preservedseveral old monuments, tablets, and brassescommemorating residents of days gone by, and inthe churchyard are some ancient tombs with curious{160}inscriptions. [Sidenote: Nonsuch Palace] Ewell,however, owes its chiefdistinction to its nearness to the site of NonsuchPalace, and the whole surrounding district is fullof memories connected with the Tudor sovereigns.Situated in the still sparsely populated parish ofCuddington, that owned a manor-house and churchat the time of the Doomsday Survey, the history ofwhich can be traced down to the sixteenth century,the property was acquired in 1539 by Henry VIII.,who added it to his Hampton Court estate. Withhis usual reckless lavishness he resolved to clearaway all the existing buildings to make room fora palace that should excel all his other residences.He enclosed sixteen hundred acres as pleasuregrounds, and brought down from London a wholearmy of architects and workmen, under whoseauspices quickly rose up a truly beautifulstructure, to which the name of Nonsuch was given,because, said its proud owner, it had no equal.It was not quite completed when Henry's careerwas cut short by death, and his son, Edward VI.,seems to have cared nothing for it. He simplyhanded it over to the care of the then Master ofthe Revels, Sir Thomas Carwardine, who evidentlyappreciated it greatly, for the story goes that whenQueen Mary came to the throne and instructed himto vacate her palace at Nonsuch, he at first refused toleave it. Indeed, he remained till the royal retainersarrived, and many unseemly quarrels took placebetween them and their servants about trivial detailssuch as the division of the produce of the royalgardens. There were armed encounters in the park{161}before her majesty took over the custody of theproperty, to which, however, she was really asindifferent as her brother had been. She actuallydecided that the best way to save herself fromfurther trouble connected with it would be to pulldown the palace and sell the materials. It wassaved from this untimely fate only through thegenerosity of the Earl of Arundel, who for love ofhis former master, who had taken such pride in it,persuaded the queen to exchange it for certain fairlands in his possession elsewhere. The transferhaving been duly arranged, the new owner, asrelated by his biographer Lyons, proceeded 'fullyto finish the house in building, reparations,pavements, and gardens in as complete and perfect sortas by the first intent and meaning of the King.' Init, thirty years after the first stone was laid, the Earlof Arundel entertained Queen Elizabeth and hercourt for a week, presenting her before she leftwith a costly set of plate. So greatly, indeed, didthe maiden queen enjoy herself at Nonsuch thatshe longed to become its owner, and when a fewyears later it passed from the possession of her hostto that of his son-in-law, Lord Lumley, she madeovertures to the latter for its purchase, which had,of course, the force of a command. During the restof her life Elizabeth was often at the palace, andfrom it many important state papers, and even moreinteresting private letters, were dated. It was therethat took place the remarkable interview with herdisgraced favourite, the Earl of Essex, after hisreturn from Holland on Michaelmas Eve 1599, that{162}is so vividly described by Rowland Whyte in anoft-quoted letter to Sir Robert Sydney, in which hesays: 'At about ten o'clock in the morning myLord of Essex lighted at Court Gate in post, andmade all hast up to the Presence, and so to thePrivy Chamber, and stayed not till he came to theQueen's Bedchamber, where he found the Queenall newly up, the hair about her face; he kneeledunto her, kissed her hands, and had some privatespeech with her, which seemed to give him greatcontentment for coming from her Majesty to goshift himself in his chamber, he was very pleasant,and thanked God, though he had suffered muchtrouble amid storms abroad, he found a sweet calmat home. 'Tis much wondered at here,' commentsthe writer, 'that he went so boldly to her Majesty'spresence, she not being ready, and he so full of dirtand mire, that his face was full of it.' It was thisvery boldness, as Essex knew full well, that was hisone chance with his angry mistress, but this time itdid not serve him long. The memory was still freshwith them both of the bitter quarrel six monthsbefore, when Elizabeth, stung to the quick by hisinsolent assertion that 'her conditions were ascrooked as her carcase,' had boxed his ears andtold him to go and be hanged, and on the verynight of his arrival at Nonsuch, after the apparentreconciliation, the earl was ordered to considerhimself a prisoner. A few days later he left thepalace in custody, and the next year he was beheadedin the Tower, all the appeals he had addressed tothe woman, to whom, in spite of all his plots against{163}her, he pretended to the last to have been devoted,having been in vain. 006ab0faaa