- Structural study of a 70 kDa tetrameric chaperone using a peptide mimic of a protein interacting partner
- Development of a force field method to solve the chiral configuration of complex natural and synthetic products using RDCs
- RNA structure refinement using RDCs measured in stretched gels, MSA, and pf1, in conjunction with SAXS data
- RDC driven NMR study of structural variation of the Vitamin D receptor upon various ligand binding (including natural hormone [1,25(OH)2D3] along with various agonists and antagonists
- Determination of the NMR structures of two phytochrome chromophore-binding domains in both ground and light excited states (collaboration with R. Vierstra's group)
- Measurement of site-specific protein backbone CSA tensors using six alignment media (collaboration with Ad Bax)
- Fast NMR methods: "HIFI-C"
- Refining an RNA structure using measured RDCs (collaboration with S. Butcher's group)
- Structure elucidation of a homodimeric protein (structural genomics target)
- NMR structural study (including protein expression and purification) of a 25 kDa protein-protein complex: (IIAMannitol)-HPr Complex of the E. coli Phosphotransferase System.
- Detecting hydrogen bonds through scalar coupling: Identification of the hydrogen bonding network in a protein by measurement of the weak (0.15-0.8 Hz) scalar couplings. Correlation with the hydrogen bond lengths (calculated from the X-ray structure).
- Protein average backbone Chemical Shift Anisotropy (CSA) tensors. Measurement of backbone amide 1H, 15N and carbonyl 13C CSA tensor components and orientation using weakly aligned proteins in a liquid crystalline medium.
- TALOS (Torsion Angles Likeness Optimized by Shifts) A database program for predicting protein backbone dihedral angles using NMR chemical shifts and residue homology.
- Correlation betweeen one-bond Ca-Cb scalar couplings and protein backbone conformation