I was wondering if you can create a similar type of AI enabled tool, but this tool looks for interior windows and automatically masks the inside area of all window frames. Please see the screenshot below for an idea.
I have had good luck with the windows program mtail. It does a very nice job of monitoring an active log. You can configure quite a few options but in general I have found the defaults work very well.
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Intravital microscopy is a powerful technique for observing various cellular processes in live animals1. Techniques for intravital microscopy via surgically implanted windows have been developed for many organs including the brain2,3,4, spinal cord5, liver6,7, lung8, skin9,10, and small intestine11,12,13, among others14. These windows can be maintained in mice for weeks at a time, allowing high resolution, chronic fluorescent imaging of the organs and tissues14. However, no such techniques have been developed for the colon to date, which is a particularly challenging target due to the high amplitude and frequency of distension and sensitivity to obstructions. There is a need for a colonic window to visualize the dynamics of the colon, such as the epithelial stem cell population, the immune response to inflammatory injury, and the neural circuitry the mediate colonic function. This new technology emphasizes chronic intravital imaging, making it possible to track the same location in colon in the same subject for multiple days.
The field of intravital imaging has been expanding the range of organs that can be observed in vivo. However, each tissue poses unique challenges due to morphology and location. While alternatives such as endoscopic techniques can provide access to certain regions, windows have three main benefits: a large field-of-view, compatibility with multiphoton imaging for deeper tissue penetration, and generating non-linear harmonic fluorescence from unlabeled tissue to provide structural context during imaing52. They also allow for easier accessibility of the target for manipulation through methods, such as optogenetic stimulation or laser ablation11,53,54. Previously, techniques have been developed to image moving tissues such as the beating of the heart55 or the expansion of the lungs8. However, colonic motility patterns and large volume fluctuations has caused longstanding difficulties for imaging due to their highly variable nature7,11. Furthermore, small animal models are limited to extremely small and lightweight devices, making most battery-powered systems too bulky for implantation. Both of these issues were overcome with the ferromagnetic scaffold, which can reversibly restrain the colon without a battery. Physical stabilization via the ferromagnetic scaffold is supported by existing tools for image post-processing to track single cells, similar to methods used in the brain, spine and heart55. The combination of our colonic window design and ferromagnetic scaffold overcome the constraints of colon physiology and can be further expanded to other moving tissues and mouse models. 0852c4b9a8
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