Since love is a sensing of something living, lovers can be distinct only in so far as they are mortal and do not look upon this possibility of separation as if there were really a separation or as if reality were a sort of conjunction between possibility and existence.[6] In the lovers there is no matter; they are a living whole. To say that the lovers have an independence and a living principle peculiar to each of themselves means only that they may die [and may be separated by death]. To say that salt and other minerals are part of the makeup of a plant and that these carry in themselves their own laws governing their operation is the judgment of external reflection and means no more than that the plant may rot. But love strives to annul even this distinction [between the lover as lover and the lover as physical organism], to annul this possibility [of separation] as a mere abstract possibility, to unite [with itself] even the mortal element [within the lover] and to make it immortal.

This unity [the child], however, is only a point, [an undifferentiated unity,] a seed; the lovers cannot so contribute to it as to give it a manifold in itself at the start. Their union is free from all inner division; in it there is no working on an opposite. Everything which gives the newly begotten child a manifold life and a specific existence, it must draw into itself, set over against itself, and unify with itself. The seed breaks free from its original unity, turns ever more and more to opposition, and begins to develop. Each stage of its development is a separation, and its aim in each is to regain for itself the full riches of life [enjoyed by the parents]. Thus the process is: unity, separated opposites, reunion.[8] After their union the lovers separate again, but in the child their union has become unseparated.


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Two and a half years ago, I moved 2,000 miles away from my beloved home in Atlanta to take a job on the West Coast. I settled in to my new home, with its view of the Pacific Ocean, and did my best to recreate the life that I'd left behind.

Early on, during one of my unpacking sprees, I found a coffee mug that dear friends had given to me before I left. It had a gorgeous sketch of the Atlanta skyline on it, dancing around the perimeter. I love coffee almost as much as I love Atlanta, and this was a treasured item to discover. In my sentimentality, I began using it almost exclusively each day.

A large piece broke off of the side, marring the beauty of the skyline. I held the sharp fragment in my palm, enormous tears welling up in my eyes. I hated the symbolism of what I was holding. I ached at the pain of losing so much of what I loved about life: my friends, my job, my home. I let the dangerously jagged edge rest on the softness of my hand and thought about how deep the wound of loss is. How mystifying grief can be, with its ebbing and flowing and refusal to ever be complete.

The late Derek Walcott, a Nobel Prize-winning writer, offered this wisdom: "Break a vase, and the love that reassembles the fragments is stronger than that love which took its symmetry for granted when it was whole."

But slowly, over time, we can open ourselves up to the awareness of the new thing that's happening in the space that was created. I believe that God works to lovingly bring us to new life, even in the wake of our loss. This is what holds me up on my worst days: the idea of God relentlessly working to bring good into the world. This is the sort of love that reassembles our fragments, and makes us into more than what we could ask or imagine.

This fragment at the University of Illinois was discovered in 1953 in the binding of a collection of sermons by Thomas Gataker (1637). The creases where it went around the spine of the book are still visible.


The spiritual journey, life itself is the path to understand this God Source. What is the Source? Where does Source reside? What is God? Who is God? How can I believe in something that is unseen, only felt? These are only a few questions that I asked myself on the Life Journey. Upon reflection, Love has always been there underneath it all. All the fear, hatred, anger, death, loss, confusion, pain, resentment, uncertainty, trauma, abuse, suffering, hope bitterness, tears, underneath it all, at the root of it, was love, unexpressed love. Love is consciousness, Love is aware of the energy given and received. When Love is expressed, more love comes, and when it is withheld or suppressed, it lessens our connection to this infinite/eternal Energy.

We find that current computational methods for estimating transcript abundance from RNA-seq data can lead to hundreds of false-positive results. We show that these systematic errors stem largely from a failure to model fragment GC content bias. Sample-specific biases associated with fragment sequence features lead to misidentification of transcript isoforms. We introduce alpine, a method for estimating sample-specific bias-corrected transcript abundance. By incorporating fragment sequence features, alpine greatly increases the accuracy of transcript abundance estimates, enabling a fourfold reduction in the number of false positives for reported changes in expression compared with Cufflinks. Using simulated data, we also show that alpine retains the ability to discover true positives, similar to other approaches. The method is available as an R/Bioconductor package that includes data visualization tools useful for bias discovery.

A sentence fragment is a string of words that does not form a complete sentence; there is a necessary component of a complete sentence missing. This missing component may be a subject (usually a noun) or a predicate (verb or verb phrase) and/or when the sentence does not express a complete idea.

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Although harboring the apolipoprotein E4 (APOE4) allele is a well known risk factor in Alzheimer's disease (AD), the mechanism by which it contributes to disease risk remains elusive. To investigate the role of proteolysis of apoE4 as a potential mechanism, we designed and characterized a site-directed cleavage antibody directed at position D151 of the mature form of apoE4 and E3. Characterization of this antibody indicated a high specificity for detecting synthesized recombinant proteins corresponding to the amino acid sequences 1-151 of apoE3 and E4 that would generate the 17 kDa (p17) fragment. In addition, this antibody also detected a ~17 kDa amino-terminal fragment of apoE4 following incubation with collagenase and matrix metalloproteinase-9 (MMP-9), but did not react with full-length apoE4. Application of this amino-terminal apoE cleavage-fragment (nApoECFp17) antibody, revealed nuclear labeling within glial cells and labeling of a subset of neurofibrillary tangles in the human AD brain. A quantitative analysis indicated that roughly 80% of labeled nuclei were microglia. To confirm these findings, cultured BV2 microglia cells were incubated with the amino-terminal fragment of apoE4 corresponding to the cleavage site at D151. The results indicated efficient uptake of this fragment and trafficking to the nucleus that also resulted in significant cell death. In contrast, a similarly designed apoE3 fragment showed no toxicity and primarily localized within the cytoplasm. These data suggest a novel cleavage event by which apoE4 is cleaved by the extracellular proteases, collagenase and MMP-9, generating an amino-terminal fragment that is then taken up by microglia, traffics to the nucleus and promotes cell death. Collectively, these findings provide important mechanistic insights into the mechanism by which harboring the APOE4 allele may elevate dementia risk observed in AD.

The fragments below have been chosen with diligence. Though with a heavy heart, fragments from Tamil, Swahili, French and other languages, had to be left out. The rationale behind picking these 27 pieces of poems is subjective. Precision, wonder, and boldness of expression, scored. Simplicity is a virtue in love poetry, unless you are John Donne. 27, for I wanted no less, but no further.

Drummers had lit a fire in the shed and they eased the skins of their tables nearer the flames to tighten them. The saffron flames, the bright grass, and the hand-woven armatures of the fragmented god who would be burnt were not in any desert where imperial power had finally toppled but were part of a ritual, evergreen season that, like the cane-burning harvest, is annually repeated, the point of such sacrifice being its repetition, the point of the destruction being renewal through fire.

Break a vase, and the love that reassembles the fragments is stronger than that love which took its symmetry for granted when it was whole. The glue that fits the pieces is the sealing of its original shape. It is such a love that reassembles our African and Asiatic fragments, the cracked heirlooms whose restoration shows its white scars. This gathering of broken pieces is the care and pain of the Antilles, and if the pieces are disparate, ill-fitting, they contain more pain than their original sculpture, those icons and sacred vessels taken for granted in their ancestral places. Antillean art is this restoration of our shattered histories, our shards of vocabulary, our archipelago becoming a synonym for pieces broken off from the original continent. ff782bc1db

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