Embedded Files
Welcome to Fluorescence Spectroscopy
Welcome to Fluorescence Spectroscopy
Fluorescence spectroscopy is one of the most powerful and sensitive techniques used in modern science to study the structure and dynamics of molecules. This site will take you on a journey to explore the science behind fluorescence, its instrumentation, applications in real-world research, and the exciting advances that are transforming the field
Principle of Fluorescence
Principle of Fluorescence
Molecules absorb light at one wavelength (excitation) and emit it at a longer wavelength (emission).
This process happens in nanoseconds.
The difference between excitation and emission is called the Stokes Shift.
Instrumentation
Light Source – Xenon lamp, LED, or laser
Monochromator – Selects the excitation and emission wavelengths
Sample Holder – Usually a cuvette
Detector – PMT or CCD to capture fluorescence signal
Types of Fluorescence Spectroscopy
Types of Fluorescence Spectroscopy
Steady-State Fluorescence – Measures constant emission
Time-Resolved Fluorescence – Observes decay over time
Fluorescence Lifetime Imaging (FLIM)
TIRF Microscopy – For surface-level imaging
FRET – Energy transfer between two fluorophores
Fluorescence Spectroscopy.pptxPage updated
Google Sites
Report abuse