Introduction:  To understand the impact of e-cigarette devices, flavours, nicotine levels and prices on adult e-cigarette users' choices among closed-system and open-system e-cigarettes, cigarettes and heated tobacco products (HTPs).

Results:  On average, participants preferred non-tobacco and non-menthol flavours most, preferred open-system over closed-system e-cigarettes and preferred regular nicotine level over low nicotine level. However, the preference varied by demographics, smoking status and the primarily used e-cigarette device and flavour. The differences in preference among products/devices were larger than the difference among flavours or nicotine levels. Participants who primarily used closed-system e-cigarettes exhibited similar preferences for closed-system and open-system e-cigarettes, but those who primarily used open-system e-cigarettes preferred much more open-system over closed-system e-cigarettes. HTP was the least preferred product, much lower than cigarettes in general, but participants living in states where IQOS is being sold had similar preferences to cigarettes and HTPs.


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Conclusions:  People are unlikely to switch to another product/device because of the restriction of flavour or nicotine level. If non-tobacco and non-menthol flavours were banned from open-system e-cigarettes, users may switch to menthol flavour e-cigarettes. Intervention strategies should be tailored to specific groups.

During fermentation, the yeast Saccharomyces cerevisiae produces a broad range of aroma-active substances, which are vital for the complex flavour of beer. In order to obtain insight into the influence of high-gravity brewing and fermentation temperature on flavour formation, we analysed flavour production and the expression level of ten genes (ADH1, BAP2, BAT1, BAT2, ILV5, ATF1, ATF2, IAH1, EHT1 and EEB1) during fermentation of a lager and an ale yeast. Higher initial wort gravity increased acetate ester production, while the influence of higher fermentation temperature on aroma compound production was rather limited. In addition, there is a good correlation between flavour production and the expression level of specific genes involved in the biosynthesis of aroma compounds. We conclude that yeasts with desired amounts of esters and higher alcohols, in accordance with specific consumer preferences, may be identified based on the expression level of flavour biosynthesis genes. Moreover, these results demonstrate that the initial wort density can determine the final concentration of important volatile aroma compounds, thereby allowing beneficial adaptation of the flavour of beer.

The qualitative sensory perception of individual and of complex mixtures of five compounds, guaiacol ('burnt note'), o-cresol ('phenolic/tar'), 4-ethylphenol (4-EP, 'leather/barnyard'), 2-iso-butyl-3-methoxypyrazine (IBMP, 'green pepper/herbaceous'), and 2,4,6-trichloroanisole (TCA, 'cork taint/ mouldy') were tested in a partially de-aromatised red wine matrix using descriptive analysis by a trained panel of eleven judges. Compounds were characterised at peri- and sub-threshold concentrations using a partial D-optimal statistical design and response surface methodology. Results indicated that complex mixtures in red wine elicit an olfactory response that could not be predicted from the attributes or descriptors of single compounds. Positive sweet/fruity attributes were more intense in solutions containing fewer off-flavour compounds. Novel findings of this study include that IBMP at sub- and peri-threshold levels shows perceptual interaction with volatile phenols at the same levels, and samples containing combinations of these compounds manifested herbaceous and burnt characteristics. Olfactory interactions of this many off-flavour compounds have not been investigated previously in one study. The findings have direct implications for wines made from cultivars that are known to contain these compounds, and add to the understanding of the behaviour and impact of very low levels (peri- and sub-threshold) of volatile phenols, IBMP, and TCA derived from various sources during winemaking.

"Because coffee beans vary, color is not an especially accurate way of judging a roast. But combined with the typical roasting temperature that yields a particular shade of brown [and some other factors], color is a convenient way to categorize roasting levels." (Lokker, 2013)

Information on spicy food flavour and intake frequency was obtained using a two-item questionnaire survey. Dietary data were collected using a validated thirteen-item FFQ. Fasting blood samples were collected and measured for total cholesterol (TC), TAG, HDL-cholesterol and LDL-cholesterol. Multivariate linear and logistic regression models were employed to examine the relationship between spicy food and serum lipids levels according to the spicy food flavour and intake frequency, respectively.

Spicy food intake was mildly associated with increased risk of abnormal TAG level, significantly associated with decreased risk of abnormal TC and non-HDL levels. Spicy food intake may be contribute to the management of lipid levels.

Results for 16 products without explicit flavour names in bar plot form; the values for three products with flavour names examined in this study (A, B and C) are included for comparison. Additional values for reference are for products with explicit flavour names as derived from Brown et al12 (see also table 1b). NYC, New York City.

Because the products examined here were obtained as a convenience sample, all study-relevant tobacco products available in NYC in 2015 were not analysed. Nevertheless, the results indicate that the samples ranged from some that were clearly overtly flavoured to others that had low overall flavour levels, and so the study-relevant range of flavour levels has probably been correctly identified. Second, the number of flavour-related target analyte chemicals was capped at 93. Nevertheless, although some added flavour compounds present in the samples may have thus escaped being reported on this work, as noted above, none of these contributed in a major way to any given sample.

With thousands of different flavours ranging from the simple sounding banana and mango to the more ambiguous such as Unicorn Puke and Stoned Smurf, and ingredients not required to be listed on the bottle, it is impossible for users to know what they are inhaling.

Further study into a number of chocolate and banana flavoured E-liquids made by several companies showed that the toxicity and immune effects were not the same between brands, and mass spectrometry analysis showed that the flavouring chemical compositions and their concentrations were different.

The effect of the addition of selected flavour enhancers (dried garlic, dried onion, caraway seeds, poppy seeds, kelp, sesame, Herbs de Provence) on levels of chloropropanediol esters (2-MCPDE, 3-MCPDE) and glycidyl esters (GE) was investigated in classic savoury shortbread with standard and reduced salt level. The changes in the amount of esters after a 2-month storage period were also analysed. Finally, selected products with reduced salt level were evaluated by consumers. The obtained results suggested that the MCPDE and GE present in bakery fat were transferred to the dough and partially decomposed during baking. Ester degradation depended on the presence of flavour additives and the amount of salt. The addition of poppy seeds or sesame accelerated the degradation, whereas onion and garlic inhibited the decomposition and, in some cases, promoted the endogenous formation of 3-MCPDE. The added salt also partially inhibited the decomposition of 3-MCPD esters but had no impact on 2-MCPDE changes. After a 2-month storage period, the level of 3-MCPDE increased in all samples that did not contain salt, while in salty products as well as for 2-MCPDE no such trends were observed. GE levels decreased after 2 months in almost all investigated samples. The consumer acceptability tests showed that the addition of poppy seeds or sesame seeds to the shortbread with reduced salt was highly evaluated by consumers. Hence, the addition of these flavour enhancers can mask the reduced salt amount, thereby lowering not only the level of sodium in the diet, but also the levels of toxic contaminants such as MCPDE or GE.

Salt, the common name of sodium chloride, due to its function in regulating flavour, taste, colour, and bacteriostatic properties, has been used as a seasoning and preservative in food since ancient times [1, 2]. In the human body, sodium is essential for the maintenance of cellular membrane potential and the absorption of nutrients in the small intestine. Its presence determines the volume of extracellular fluid, thereby maintaining blood pressure [3]. However, excessive consumption of sodium has been associated with negative health effects, including most of all cardiovascular disease and hypertension, stroke, stomach cancer, kidney disease and bone demineralization [3,4,5]. Therefore, numerous national and international organisations have introduced recommendations and actions for lowering sodium chloride levels in foods. Currently, the WHO/FAO provides a guideline of less than 2 g of sodium intake per day [5].

In most European countries, a major source (nearly 40%) of sodium in the diet comes from cereal products such as bread, biscuits, breakfast cereals, pastries, cakes, and cereal snacks [2, 3]. In bakery products, apart from regulating the flavour, salt improves the texture and the water-binding capacity of the dough. Due to its ionic nature it also controls the water activity of product and results in an increased shelf life [6].

On the other hand, in the case of some spices such as garlic or onion, their presence in food that is subjected to heat treatment may lead to an interaction with fat which results in an increase in the level of free 3-MCPD [21]. Increased levels of 3-MCPD esters in deep-fried garlic and onion have also been reported recently [22]. However, no such research has been performed for chloropropanediol esters and glycidyl esters in bakery products with the addition of spices or other flavour additives. There is also a gap in the data concerning changes of esters during storage of bakery products with different salt levels. 2351a5e196

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