Fish act aggressively towards their mirror image suggesting that they consider it another individual, whereas in some mammals behavioural response to mirrors may be an evidence of self-recognition. Since fish cannot self-recognize, we asked whether they could distinguish between fighting a mirror image and fighting a real fish. We compared molecular, physiological and behavioural responses in each condition and found large differences in brain gene expression levels. Although neither levels of aggressive behaviour nor circulating androgens differed between these conditions, males fighting a mirror image had higher immediate early gene (IEG) expression in brain areas homologous to the amygdala and hippocampus than controls. Since amygdalar responses are associated with fear and fear conditioning in other species, higher levels of brain activation when fighting a mirror suggest fish experience fear in response to fights with a mirror image. Clearly, the fish recognize something unusual about the mirror image and the differential brain response may reflect a cognitive distinction.

The method for nuclei segmentation in fluorescence in-situ hybridization (FISH) images, based on the inverse multifractal analysis (IMFA) is proposed. From the blue channel of the FISH image in RGB format, the matrix of Holder exponents, with one-by-one correspondence with the image pixels, is determined first. The following semi-automatic procedure is proposed: initial nuclei segmentation is performed automatically from the matrix of Holder exponents by applying predefined hard thresholding; then the user evaluates the result and is able to refine the segmentation by changing the threshold, if necessary. After successful nuclei segmentation, the HER2 (human epidermal growth factor receptor 2) scoring can be determined in usual way: by counting red and green dots within segmented nuclei, and finding their ratio. The IMFA segmentation method is tested over 100 clinical cases, evaluated by skilled pathologist. Testing results show that the new method has advantages compared to already reported methods.


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View the Official Rules. Entrants are responsible for complying with the Official Rules or may result in disqualification. This contest is for images of Arizona wildlife. All photos must depict wildlife native to and found in Arizona, and all photos must have been taken in Arizona settings.

As long as there is an Internet, there will be trolls. Nothing I say will change that. You might ask yourself this question though, if you are one of the folks complaining about forced perspective making fish look bigger than they actually are.

The good news is there are still fish to catch, there are lots of folks paying for fishing licenses, there are slightly less folks working with their local chapters of TU, CCA, or support organizations like ASF, CCF, BTT, etc, but thank goodness they are working to conserve & protect the watersheds, habitats and species for new generations of anglers.

And, I agree with you 100%; it is as much, if not more, the friends I have made through fishing that I treasure beyond that 27-pound bonefish I caught in Lake Minnetonka last winter, ice fishing. Why? Because they laugh and know it was only 23-pounds and was actually taken on worms in Lake Erie last summer.

First of all Fantastic fish and Photography! Second, my guess is the trolls never caught a fish of this magnitude. And last but not least have they seen a super model or a actress without all the make up!!

you know what? as a fly fishing guide in colorado, i can relate to when you said that you were as happy taking the pic as you would have been catching that brown. i get as excited seeing others hook up as i do when its me. and i love that pic of the baby brookie with a huge hopper in his mouth as much as the pic of the brown! (well, almost) it has its own story too, just like the pic in question.

A great fish, to be sure. My complaint with almost all modern fish photography is the amount of time the fish spends out of the water to achieve these magazine-cover-style shots. In my mind, a photograph of a fish should be a small memento to help recall the fish and share the moment with friends. If you can get a photograph of that quality without keeping the fish out of the water more than a few seconds, more power to you. If the fish is sacrificed to get the hero shot, then count me out.

Our Fly Fishing club has just adopted a policy to not take pictures of fish out of the water. We are all fishing a valuable resource that should be protected in every way possible.

Congrats on that fish, however.

My brother and I have had discussions about the new trend in fly fishing photography (enhanced perspective / forced perspective). We agree that the pictures are more interesting than the old school hero shots. They look more alive, less wooden and stiff. The fish always looks better. You can see the details, the spots, the colors; all the things that make the fish beautiful, much better with a shot that is zoomed in on the fish.

One thing from my perspective: I love the shots during the fight. While most of my fly fishing is currently from a boat (inshore salt water), this is easier, but the series of pictures with bent rod, excited smiles, then one of the fish, tell a much better story. I know this is impossible if fishing solo.

Following behavioural trials, blood samples were collected and plasma testosterone and 11-ketotestosterone were measured using commercially available kits (Parikh et al. 2006). Brains were removed and frozen in a mounting medium (Tissue Tek) on dry ice. Brains were sliced coronally in 300 m sections using a cryostat and mounted on glass slides. A frozen stage (Physitemp) viewed through a dissection microscope was used for microdissection, performed with a modified 27G needle with an internal diameter of 190 m following an established protocol (Korzan et al. 2000). Brain atlases from A. burtoni (Fernald & Shelton 1985; Burmeister et al. 2009) and from other fish species (e.g. Reiner & Northcutt 1992) were used to target the Cce, Dl, Dm and POA and for all of these brain areas, the entire nucleus was collected and treated identically (e.g. multiple subdivisions of each nucleus are included). Micro-dissected tissue was collected in a lysis buffer (RNeasy, Qiagen Inc.).

The original artwork for most images on this web site was prepared by Ellen Edmonson and Hugh Chrisp as part of the 1927-1940 New York Biological Survey conducted by the Conservation Department (the predecessor to today's New York State Department of Environmental Conservation). Permission for use of these images can be requested from the New York State Department of Environmental Conservation by filling out a form available on the agency usage and policies web page

Caledonia is very well known for challenging its adversaries while seemingly creating picturesque views throughout. Discover the images below for a taste of what Mike Strantz calculated architecture has to offer.

We invite you to share photos of your experiences fishing, hunting, and enjoying the outdoors here in Washington. We also welcome fish and wildlife photography submissions. Let's show the world that Washington is a great place to recreate and volunteer outdoors!

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This artwork is in public domain in its country of origin {{::country}} and other countries and areas where the copyright term is the author's life plus 50 years or less. If you are a copyright owner of this artwork, or his/hers legal representative, and you do not agree that this artwork is in public domain, please let us know wikipaintings@gmail.com 


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This artwork is public domain by a decision of its author. If you are a copyright owner of this artwork, or his/hers legal representative, and you do not agree that this artwork is public domain, please let us know wikipaintings@gmail.com 


 WikiArt.org allows unlimited copying, distributing and displaying of the images of public domain artworks. Artworks protected by copyright are supposed to be used only for contemplation. Images of that type of artworks are prohibited for copying, printing, or any kind of reproducing and communicating to public since these activities may be considered copyright infringement. ff782bc1db

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