Plant tissue culture generally refers to in vitro culture of all plant parts under sterile conditions. Basic facilities are must in the laboratory where tissue culture technology is to be performed. This includes general cleaning and media preparation, sterilization, storage, sterile transfer, observation / data acquisition, and environmentally controlled incubator or culture room areas. The most important work area is the Cultural Transfer Office, where core activities take place. Plant tissue culture should be cultured for a specified period of time under well-controlled temperature, humidity, airflow, and light quality. Plant culture techniques require a variety of organic and inorganic chemicals to prepare the culture medium. The growing room is an equally important area where plant cultures are maintained under controlled environmental conditions for optimal growth. After in vitro culture of plants they are transferred to the pots. The potted plants are immediately transferred to the greenhouse or growth cabinets and are maintained under proper conditions of light, temperature, and humidity. Research and training organization

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