Research

STED microscopy

Visualizing 3D structure and dynamics at the molecular scale is a current and critical need in biomedical research. Many sub-cellular features, for example the morphology of many organelles or the 3D organization of chromatin, cannot be resolved by conventional light microscopy. Improving the resolution of light microscopy has therefore been an urgent need in biological research for many decades.

STED microscopy is a laser-scanning microscopy technique that, in addition to an excitation laser, uses a separate laser beam to prevent fluorescence emission through STED. To quench fluorescence only in the periphery of the excitation focus, STED microscope shape the focal spot of the depletion beam to have zero intensity at its center. This is usually achieved by using phase masks in a conjugate plane of the objective's back pupil and/or adjusting the polarization.

Currently, we are focusing on the development of fast easySTED and adaptive STED microscopy.

Polarizing Confocal microscopy

Circularly Polarized Illumination Confocal Microscopy (CPICM) is a confocal microscopy with a rotating analyzer to measure the three-dimensional orientation of polarized fluorescence emitters.

We design and implement this novel microscopy systems, and devleop computational algorithms for anlayzing the measured signals. Our resarch aims to visualize three-dimensional director fields in liquid crystal.

Spectrally encoded confocal microscopy

With the increase in semiconductor wafer size to 12 inches, surface warpage is inevitable issue that critically impacts manufacturing quality. Therefore, advanced inspection technique is required for non-contact, fast, and accurate measurement. To meet this demand, we are developing a Spectrally Encoded Confocal Microscopy (SECM) to measure not only wafer warpage but also surface defects. This technique can achieve confocal images without point scanning mechanisms.

Point Spread Function engineering

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