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The conflict between India and Pakistan over the Kashmir problem still seems to be an obstacle in their relations. It has been 58 years since the dispute passed, but there is no final agreement that can be accepted by both parties. If 1947 is the independence day of India and Pakistan, then this year is the beginning of the suffering of the people of Kashmir. Pakistan, as the party that has the right to make Kashmir part of its territory, feels the need to continue to raise this issue even though it has to develop nuclear power.

Phylogenetic placement of Melicope stonei K.R. Wood, Appelhans & W.L. Wagner based on four nuclear and two plastid markers (modified from Appelhans et al. 2014c). The phylogenetic tree only shows the Hawaiian radiation of Melicope. The terms Apocarpa, Cubicarpa, Megacarpa and Pelea refer to the former Hawaiian sections of Melicope/Pelea (Hartley & Stone 1989). The support values are displayed above the branches and the first value represents the Bayesian posterior probability values (pp), followed by the bootstrap values (bs) from the Maximum Likelihood analysis.

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Many technologies have been developed over the past several decades in an attempt to improve examination of the oral cavity when screening or potentially diagnosing oral pre-cancer or cancer [7, 14, 15]. Several of these are aids to visual inspection of the oral cavity, effectively increasing the contrast between dysplastic or malignant tissue and normal tissue. These include toluidine blue staining [16] for detecting the increased nuclear content in malignant lesions and use of acetic acid with blue light to produce chemiluminescence, which can potentially improve visualization of oral lesions [17]. While these are potentially simple ways to improve the standard visual screening, studies report poor specificity of these techniques and moreover they are not validated with gold standard biopsy data [7]. Other potential techniques include autofluorescence imaging of metabolic intermediates that indicate cancer phenotypes, broad-spectrum reflectance and scattering that can probe architecture [18] and optical coherence tomography (OCT) [19]. While promising, these techniques have had limited acceptance and require custom instrumentation that is often unavailable at the point of care.

A screening tool that has been successfully evaluated across a range of medical setting is brush biopsy [20, 21], which involves minimally invasive sampling of the suspicious region with a brush followed by slide preparation and pathologist interpretation with a transmission light microscope. Cytological information gathered from these samples can indicate cellular changes including nucleus-to-cytoplasm ratio and nuclear structure. This technique can be a useful aid for differentiating benign lesions from potentially cancerous lesions, enabling a frontline health worker to make a more effective referral to a central facility for final diagnosis. However, this technique has not been available in peripheral clinics due to challenges associated with the need for microscopes and trained personnel to interpret the images. ff782bc1db