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The 2024 version of MediaTek Universal Tool V5 boasts an intuitive user interface, ensuring both novice and experienced users can navigate the tool effortlessly. The streamlined design enhances the overall user experience.

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In 2008, the WHO recommended universal early antiretroviral treatment for HIV-infected children aged less than 12 months to improve their survival, irrespective of clinical status or symptomatology [7], [8]. In 2010, these guidelines were revised to include therapy for all children less than 24 months of age [9]. To improve early access to pediatric antiretroviral therapy in resource-limited settings, it is crucial to assess public health strategies for early HIV infant diagnosis (EID) at the community level [10].

While adult HIV counseling and testing practices have been scaled up in low income countries, the routine offering of HIV testing to pediatric patients in such settings often occurs too late [2], [11], [12]. Only 6% of infants born to HIV infected mothers had access to EID in 2009 [6]. EID including both children exposed and non-exposed to PMTCT interventions is the main operational challenge in Africa [13]. EID requires active investment in key areas such as training and support for providers, improvement of laboratory tools and referral networks. The use of dried blood spot (DBS) specimens collected on filter paper can improve testing networks for early infant diagnosis as in South Africa, but still needs to be generalized in many African countries [14], [15].

Sub-samples of parents who refused and accepted infant HIV testing were interviewed at each pediatric health center by a public health sociologist, using a semi-directive grid tool to further understand the local interpretation of the acceptability of HIV testing in children, the mother's perceptions of EID, the reasons for accepting or refusing HIV testing for their infant, and the constraints and difficulties related to HIV early infant diagnosis.

Conceived and designed the experiments: VL HB MTK MO. Performed the experiments: MTK HB MO CAB DKE. Analyzed the data: HB CN VL MO. Contributed reagents/materials/analysis tools: HM CN VL. Wrote the paper: CN VL. 006ab0faaa

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