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The song begins with Joseph warning about the dangers of sniffing cocaine and the detrimental effects it has on those who engage in its use. The chorus emphasizes how cocaine "gets them off" or alters their state of mind, making them weak and susceptible to its control.

In the second verse, the lyrics highlight the neglectful behavior of those addicted to cocaine. They prioritize the drug over basic personal hygiene, clothing, and cleanliness. The addiction consumes them to the point where they prioritize cocaine over taking care of themselves and maintaining their well-being.

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The fourth verse mentions a father who leaves behind a truck for his child, symbolizing the potential for a better future or opportunity. However, the addiction hinders the individual from making use of the gift, pushing them towards criminal activities rather than pursuing a legitimate path.

The fifth verse expresses the disappointment of a mother who worked hard to send her child to school, hoping they would learn the right values and morals. However, the child's addiction leads them to disrespect education and engage in disruptive behavior.

The final verse questions the addicted individual's thought process and the example they set for the younger generation. It raises concerns about the negative influence they have on others and what the youth may think of them.

This is aided by the spread of certain technologies. Rich families have solar panels, due to frequent power outages, that allow them to enjoy foreign media uninterrupted. A lucky minority have mobile phones. While the North Korean regime jams foreign broadcasts, some make it through, especially in border areas. And USB drives have become increasingly popular in North Korea, for being easier to smuggle than previous digital media like DVDs.

Illinois researchers developed a soft growth medium for stem cells that allows them to culture homogenious pluripotent colonies, without expensive growth factor chemicals. The team: front row from left, graduate students Farhan Chowdhury and Yanzhen Li and visiting scholar Tamaki Yokohama-Tamaki; back row from left, graduate student Yeh Chuin Poh; Tetsuya Tanaka, professor of animal sciences; and Ning Wang, professor of mechanical science and engineering.

In a paper published in the journal PLoS One, the researchers demonstrated that culturing mouse embryonic stem cells (mESCs) on a soft gel rather than on a hard plate or dish keeps them in their pluripotent state, a ground state with the ability to become any type of tissue. The soft substrate maintains homogeneous pluripotent colonies over long periods of time - without the need for expensive growth chemicals.

The difficulty of maintaining mESC colonies that are homogeneously pluripotent has been one of the main obstacles in stem cell research. Pluripotent stem cells spontaneously differentiate, beginning to turn into specialized tissue types such as skin or muscle. Scientists use chemicals called growth factors to keep mESCs in their unchanged state, but even then it's not long before the culture is a mixture of cells in various stages of differentiation, with diverse gene expression and morphologies.

"If we start from a homogenous population of undifferentiated cells, differentiation toward the tissue of our interest might become much more homogenous than we've been able to achieve," said Tanaka, who also is affiliated with the U. of I. Institute for Genomic Biology. "So then, in generating a specific cell type - the main application of pluripotent stem cells - I think that there is an advantage to having a homogeneous culture to start with."

After noticing that pluripotent mESCs tend to stick together in round colonies while cells on the colony edges in contact with the rigid growth plate tend to differentiate more quickly, the team decided to focus on mESC mechanics rather than chemistry. Since stem cells are 10 times softer than mature cells, the researchers wondered if the mechanical forces between the plate and the cells were spurring differentiation. Wang and Tanaka's earlier research found that even small mechanical forces could be used to direct cell differentiation; could mechanics also hamper differentiation?

The team did side-by-side comparisons of mESCs grown on a traditional medium with growth factor and mESCs grown on a soft gel with the same stiffness as the cells, both with and without growth factor. They found that cells grown on the soft gel had greater homogeneity and pluripotency, even without growth factor, and even more than three months and 20 passages later.

Next, the researchers want to try their soft-substrate method with induced pluripotent stem cells (iPSCs), mature cells that have been genetically reprogrammed to a pluripotent state. These cells hold a lot of promise for medical applications, but are notoriously hard to culture and not as well understood as embryonic cells.

"We can try culturing mouse iPSCs on the same soft substrate and see if the same benefit applies to achieve homogenous stem cell cultures," Tanaka said. "If that's the case, the impact would be significant."

This work was supported by the National Institutes of Health, the United States Department of Agriculture, and the University of Illinois. Co-authors were graduate students Farhan Chowdhury, Yanzhen Li and Yeh-Chuin Poh, and visiting scholar Tamaki Yokohama-Tamaki.

Shouldnt come as a surprise as we've had tons of red flags, but so so sooooo bummed and saddened to hear how dismissive dax was and how eager they seemed to reduce standing for basic human rights as being 'cancel culture ' or some shit?!? As if jk Rowling was just a victim of a rabid twitter mob rather than her being a vile TERF and trans hating pos.

Cancer cells respond to matrix mechanical stiffness in a complex manner using a coordinated, hierarchical mechano-chemical system composed of adhesion receptors and associated signal transduction membrane proteins, the cytoskeletal architecture, and molecular motors. Mechanosensitivity of different cancer cells in vitro are investigated primarily with immortalized cell lines or murine derived primary cells, not with primary human cancer cells. Hence, little is known about the mechanosensitivity of primary human colon cancer cells in vitro. Here, an optimized protocol is developed that describes the isolation of primary human colon cells from healthy and cancerous surgical human tissue samples. Isolated colon cells are then successfully cultured on soft (2 kPa stiffness) and stiff (10 kPa stiffness) polyacrylamide hydrogels and rigid polystyrene (~3.6 GPa stiffness) substrates functionalized by an extracellular matrix (fibronectin in this case). Fluorescent microbeads are embedded in soft gels near the cell culture surface, and traction assay is performed to assess cellular contractile stresses using free open access software. In addition, immunofluorescence microscopy on different stiffness substrates provides useful information about primary cell morphology, cytoskeleton organization and vinculin containing focal adhesions as a function of substrate rigidity.

But many companies neglect the soft costs of turnover. Especially in knowledge-intensive domains with low capital intensity like software development, investment management, accounting, teaching, or public relations, these soft costs matter. Each employee leaving their job takes with them a unique set of knowledge, a professional network, and established trust with clients and colleagues. The costs of rebuilding these qualities are significant, if impossible to quantify.

The good news, however, is that auditors who valued learning and who reported to work in a learning culture had the lowest turnover rate in our study. We found that turnover is, to some extent, a question of fit. Auditors who value learning but feel their environment does not, left after less than 2.5 years on average; but auditors whose own values match those of their firm left after an average of 3.3 years, a significant difference.

To dive more deeply into what it takes to create a learning culture, we interviewed several top-level auditors, such as members of the board of management, partners responsible for HR, strategy, and quality. This group of people has been involved in a multi-year effort to build a learning culture within their firm, and they shared the following wisdom:

Becoming aware of the answers to these questions might not only help you to decrease turnover behaviour. In fact, the firm we worked with in creating a learning culture also observed an increase in the wellbeing of their employees: they reported to be less stress and to experience more professional development and purpose in their work!

The cause of young-onset dementia is often assumed to be genetic. Researchers from Maastricht University (UM) and the University of Exeter have now identified 15 factors associated with an increased risk of developing dementia at a young age, some of which people can influence themselves.

Work on popular culture and IR has identified various ways in which films, television series, popular literature, and other cultural artifacts (often in the science fiction genre) might reflect and even affect real-world politics. It stands to reason that video games could have similar effects, but with few notable exceptions, these products have received much less attention than those in more established media. I will more systematically consider how video games might affect our political world in my next post. For now, I want to focus on TotK. 17dc91bb1f