As suggested, we used publicly available RNA-seq data to evaluate whether inactivated SERPINE3 genes are still expressed. RNA-seq data of eyes, where SERPINE3 expression is expected, is available for several bat species. We blasted the full-length SERPINE3 sequences as annotated by TOGA against these RNA-Seq data sets and obtained a very low number of aligning reads (

In this section, we derived, from the eye and hand movements' data, a second internal measure of executive functioning, in this case, inhibitory control. When the eyes fixate on a new item, it could be either the next item in the sequence or not, i.e. it could be a Correct or a False Detection of the target. In the latter case, the hand has to avoid following the eye and wait until the correct item is found. This behaviour is evident when aligning all the paths explored by the hand after fixating a new item (Fig. 5B,C, Fig. S3). The spatial distribution of these paths shows that, when a correct item was identified, the hand moved directly towards the target (Fig. 5B). When a false detection occurred, the hand stayed still (Fig. 5C) or moved in other directions (Fig. S3) showing an inhibition of early motor actions. In order to quantify this behaviour, we estimated the displacement in the direction of the new item, for Correct and False detections, and for TMT-A and TMT-B separately.


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