Cyclotella atomus has a flat valve face with short striae arranged around the margin on the valve. A fultoportula can be seen toward the center of the valve (but slightly off center) appearing as a dark dot. Marginal fultoportulae occur on every fourth to fifth costa and can be seen in deep focus as dark spots.

The 15 response plots show an environmental variable (x axis) against the relative abundance (y axis) of Cyclotella atomus from all the stream reaches where it was present. Note that the relative abundance scale is the same on each plot. Explanation of each environmental variable and units are as follows:


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The 15 response plots show an environmental variable (x axis) against the relative abundance (y axis) of Achnanthidium atomus from all the stream reaches where it was present. Note that the relative abundance scale is the same on each plot. Explanation of each environmental variable and units are as follows:

The 15 response plots show an environmental variable (x axis) against the relative abundance (y axis) of Surirella atomus from all the stream reaches where it was present. Note that the relative abundance scale is the same on each plot. Explanation of each environmental variable and units are as follows:

Diatomaceous earth or diatomite is a fossil rock deposit of diatoms made up of silica and other minerals. A distinguishing feature of diatoms that placed them in the single class of microalgae Bacillariophyceae, is the frustule, a transparent, hard-shelled cell wall. It's interesting to note that the diatom has specific proteins and enzymes for heavy metal detoxification and can intake and store more heavy metals in its frustule. Consequently, an attempt has been made in this study to determine the bioaccumulation of metals in the frustules of the diatom. Hence, a centric diatom was isolated from the freshwater sample collected from the Adyar River, Chennai, Tamil Nadu. The diameter of the cell was 5-7.5 m and 20-23 striations with radial arrangement. A single, dark off-center fultoportula and marginal fultoportula on the striae are found in the diatom. Additionally, one rimoportula between two marginal fultoportula distributed on the striae between the costa was also seen. As a result, the isolated diatom was morphologically identified as Cyclotella atomus Hust. Simultaneously, the bioaccumulation study reveals that the Titanium (Ti) was found accumulated in the frustules of the diatom incubated in the Ti-supplemented culture medium based on the scanning electron microscope-energy-dispersive X-ray analysis (SEM-EDAX). Therefore, the biogenic accumulation and fabrication of Titanium frustules in diatom have advantages in enhancing the efficiency of solar cells.

Cyclotella atomus grows well at 15-20C and occurs at maximum abundance at the upper end of this range, although it can also tolerate higher temperatures. It has been recorded in the Great Lakes drainage in spring and summer (Klarer and Millie 1994, Poulickova 1993, Stoermer and Ladewski 1976).

Cyclotella atomus can tolerate turbulence as well as frequent osmotic stress. It has been recorded from fresh, brackish, and saltwater. In Lake Michigan, it occurs in littoral areas that have abnormally high chloride levels. It has made up 8.3% of the diatoms in the Lake Ontario and Oswego River regions during periods of high chloride concentration (Hakansson and Clarke 1997, Makarewicz 1987, Mills et al. 1993, Stoermer and Yang 1969, Tanimura et al. 2004).

Citation: von Alvensleben N, Stookey K, Magnusson M, Heimann K (2013) Salinity Tolerance of Picochlorum atomus and the Use of Salinity for Contamination Control by the Freshwater Cyanobacterium Pseudanabaena limnetica. PLoS ONE 8(5): e63569.

Industry aims for microalgae cultivation at various power-stations in Australia for CO2 and NOx remediation from flue gas with parallel production of value-adding biochemicals. However, these sites differ in the water quality for cultivation. A cosmopolitan marine microalga, Nannochloris atomus Butcher (Chlorophyta, synonym for Picochlorum atomus (Butcher) Henley [4]), has a suitable lipid and protein content for aquaculture [5], [6], high biomass production and a potentially broad tolerance to variations of salinity [7], [8]. However, the influence of salinity on growth patterns, nutrient requirements and biochemical profiles below 36 ppt, which are commonly encountered at potential production sites, have to date not been determined. Establishing species-specific growth parameters will identify optimal inoculation cell numbers and culture durations for achieving highest biomass productivity in the shortest possible timeframe. Understanding species-specific daily nutritional requirements will ensure minimal environmental impact (e.g. eutrophication through discharge of nutrient-rich harvest water effluent [9]), whilst also minimising expenses associated with fertilisation.

Given the potential importance of P. atomus in aquaculture, this study firstly aimed to determine the influence of salinity on growth, nutrient utilisation, biomass and lipid production and effects of nutrient limitation on biochemical profiles to determine end-product choice and industrial-scale cultivation protocols. Additionally, the effectiveness of salinity manipulations for contamination control of the freshwater cyanobacterial contaminant P. limnetica was investigated.

It is shown that salinity had no effect on P. atomus growth and nutrient utilisation (except at 11 ppt for the latter) and had only a marginal effect on total lipid at 2 ppt and carbohydrate at 8 ppt, respectively, under nutrient-replete conditions. Nutrient status, however, significantly affected total lipid and fatty acid profiles, carbohydrate and protein contents. It is further shown that salinity can be used to control the establishment of P. limnetica.

Growth of Picochlorum atomus was determined daily using turbidity, from triplicate 250 l samples per culture for 20 days and obtained data were transformed to cell numbers and dry weights as described above. Specific growth rates [], (eq. 1) were calculated from culture cell numbers [31], as were the derived parameters divisions per day and generation time [days]. Biomass productivies were determined using equation 2 (modified from Su et al. [32]).

Irrespective of salinity, Picochlorum atomus exhibited growth patterns typical of aerated batch cultures [11]. The data established that P. atomus is a euryhaline microalga tolerating freshwater to marine salinities without adverse effects on growth and biomass productivities.

The observed growth patterns for P. atomus have direct implications for industrial cultivation, as optimal productivities are achieved in relatively dilute cultures for a brief period. Harvest effort and costs inversely correlate with culture cell densities. Consequently, future studies should investigate whether higher inoculation densities and/or semi-continuous culturing would improve biomass yield and overall productivity. In addition, the accumulation of microalgal/bacterial exudates and their effects on culture development require further investigation, as these may affect water treatment and recycling capacity on industrial-scales.

Initial nitrate uptake by P. atomus was similar at all salinities (except 11 ppt) and comparable to Nannochloris maculata [47]. With the exception of cultures at 11 ppt, patterns of nitrite secretion until day 10 can be grouped into high (28 and 36 ppt), intermediate (18 and 8 ppt) and low (2 ppt) salinity patterns, where medium nitrite was highest in low salinity cultures. Medium nitrate depletion resulted in expected nitrite resorption as intracellular nitrogen stores became depleted [33]. Nitrogen fluxes can provide insight into possible osmoregulatory mechanisms, often reflected in changes of biochemical profiles. The production of osmoregulatory solutes, such as proline in response to hyperosmotic stress has been reported for Nannochloris sp. [48], which would require higher nitrogen provisions. However, despite the variable nitrite secretion, total nitrogen uptake patterns (except for 11 ppt) were not significantly different. This may indicate that higher nitrite secretion in the lower salinity cultures was potentially due to a slight swelling of cells, increasing cell surface area [49], thereby increasing nitrate uptake. In contrast to nitrate [50], nitrite cannot be stored and is cytotoxic in higher concentrations [11]. Reduction of nitrite to ammonium is limited by nitrite reductase activity (a reaction directly linked to photosynthesis and under circadian control [51]). Thus, when nitrate reduction exceeds the reducing capacity of nitrite reductase, nitrite is secreted.

As for nitrate uptake, initial phosphate uptake across all salinities was comparable to Nannochloris maculata [47] and uptake rates were comparable to Chlorella stigmatophora, showing potential for urban waste-water remediation [56]. Remediation studies using Neochloris oleabundans have shown phosphate uptake to correlate with increasing medium phosphate availability [54]. Consequently, further studies should investigate P.atomus phosphate uptake when exposed to higher concentrations.

The N:P ratio of P. atomus was similar to Nannochloris atomus [57]. The N:P ratio decreased over culture time as nutrient availability per cell decreased and cell numbers increased. Downstream effects of the decreased N availability resulted in reduced total protein contents (Fig. 8).

Nutrient status also affected total carbohydrate and protein contents which increased and decreased, respectively, following nutrient limitation. Both carbohydrate and protein contents were similar under nutrient-replete conditions and slightly higher than reported for Nannochloris atomus under nutrient limitation [58]. Similar patterns of protein decrease and concurrent carbohydrate increase as a result of nutrient depletion have been observed in a number of microalgal species e.g. Chlorella vulgaris and Scendesmus obliquus [59], as N-limitation prevents the synthesis of proteins, channelling the photosynthetically acquired carbon into storage. Nutrient-replete Picochlorum atomus has been shown to be a promising replacement for Nannochloropsis oculata in aquaculture for grouper larval rearing [6], which is rapidly expanding, and already one of the most valuable aquaculture species in Southeast Asia [66]. 2351a5e196

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