We investigate how cells sense and repair DNA and RNA damage, and how cancer cells exploit these pathways to survive therapy. Using CRISPR-based functional genomics, molecular and biochemical approaches, we study how poly(ADP-ribose) signaling and RNA-binding proteins regulate DNA repair, R-loop dynamics, and resistance to PARP inhibitors. We also discovered that the chemotherapeutic drug 5-fluorouracil kills cancer cells through RNA damage during ribosome biogenesis, redefining its mechanism beyond DNA damage. By revealing vulnerabilities hidden within nucleic acid damage signaling, our goal is to inspire more precise and effective cancer therapies. Students who are passionate about nucleic acid and cancer research are welcome to join our team!
本實驗室研究細胞如何偵測並修復 DNA 與 RNA 損傷，以及癌細胞如何利用這些機制在治療中存活。我們運用 CRISPR 功能基因體學，分子和生化方法，探討 RNA 結合蛋白與 poly(ADP-ribose) 訊號如何調控 DNA 修復、R-loop 動態，以及 PARP 標靶治療的抗藥性。此外，我們發現化療藥物 5-FU 是透過造成 RNA 損傷與核糖體生成錯誤來殺死癌細胞，顛覆了其以 DNA 損傷為主的傳統觀點。透過解析核酸損傷的核心訊號，我們致力找出癌症的致命弱點，發展更精準有效的治療策略。歡迎對核酸與癌症研究充滿熱忱的同學加入我們的研究團隊。
2025/Nov - Hsiang-Pei (Gina) Chen joined the lab as a research assistant. Welcome!
2025/Oct - Chun-Yu Yen joined the lab as the first research assistant. Welcome!
2025/Aug - Cheng-Chuan Huang joined the lab as the first master student. Welcome!
2025/Jun - Wen-Chien (Claire) Shih join the lab as the first PhD student. Welcome!