Open Valve to CO2 tank.

Cool stage until completely frozen.

Cut straight across the cerebellum to the brain stays level and upright.

Apply 1% Ethanol to frozen stage as the base.

When ethanol is halfway frozen, add a layer of 30% sucrose to the base.

When sucrose is halfway frozen, place brain so that the dorsal side will face towards the blade.

Add more sucrose around the tissue and allow to freeze.

Manually lower or raise the stage so that the tissue is below where the blade will run.

Place blade in its designated space and tighten screws to hold the blade in place.

Obtain a brush to handle the tissue and sectioned dish full of distilled H2O to place tissue.

Begin cutting tissue into sections of the desired thickness (50 microns) and fully extend cutting arm for automatic adjustments.

When done cutting, remove blade, wash with soap and water, then dry. Coat blade in oil and store until next use.

For mounting, place one section in dish of water, float to the top and place slide under tissue in water.

Using a brush, guide the tissue to its spot on the slide.

When done mounting, allow your tissue to dry before staining.