Applicants will be notified soon
Course description
Cellular signaling is carried out by dynamic multi-molecular complexes that may coalesce, split apart, relocate, gain and lose individual regulatory proteins. The state of these complexes can be switched by post translational modifications, and once the signal has been transmitted and complexes are no longer needed, they can be fully dismantled. Despite their central importance, for the most part these regulatory complexes are poorly understood. A protein’s modular architecture determines the types of interactions it can establish. Many of these interactions are mediated by flexible or “natively unstructured” regions that code important functions through modular elements termed “linear motifs”. This Workshop will present a variety of bioinformatics tools that can be used to explore a protein’s functional architecture as a tool towards predicting and testing its possible functions and interactions.
Hands-on workshop (24 hours). Targeted to undergraduate students, graduate students or postdocs with a molecular biology background.
Organizers:
Toby Gibson (EMBL,Heidelberg) and Lucía Chemes (IIB-UNSAM)
Speakers:
Toby Gibson (EMBL, Heidelberg)
Lucía Chemes (IIB-UNSAM)
Gonzalo de Prat Gay (FIL)
Ignacio Sánchez, (FCEN, UBA)
Teaching team:
Toby Gibson (EMBL, Heidelberg)
Lucía Chemes (IIB-UNSAM)
Hugo Samano (EMBL, Heidelberg)
Nicolás Palopoli (UNQ)
Juliana Glavina (FCEN, UBA)
The course will be taught in english
Course limited to 25 students. More information: lchemes@iib.unsam.edu.ar
Fee: Free for UNSAM students, $300 for external applicants*.
Computers will be provided.
The course will have a final examination when required.
*Two fellowships will be available for students who are not able to pay the registration. If you need a fellowship, please indicate it in the box provided upon registration
SCHEDULE:
Tuesday, November 21st
9:00-9:30 am: Registration
9:15 am-12:30 pm. Course Intro (Aula 1).
9:30-10:15 am: Welcome. Presentation of teachers and students.
10:15-11:00 am: Course Introduction I (Lucia Chemes).
Coffee Break 11:00-11:30 am
11:30-12:30: Lecture by Toby Gibson. Title: warm up and experimental tips.
12:30-1:30 pm: Lunch on own
1:30-3:00 pm: Practical Session 1: Protein databases and related annotation resources (UNIPROT, Pfam).
Coffee Break 3-3:30 pm
3:30-5 pm: Practical Session 2: Tools and resources for the analysis of the structural architecture of a protein (PDB, InterPro, TMHMM).
Wednesday, November 22nd
9:00-11:00 am: Practical Session 3: Analysis and prediction of intrinsically disordered regions (JPred, IUPRED, Anchor, Disprot, MobiDB).
Coffee break at IIB-INTECH 11-11:30pm
11:30hs-12:30hs: Lecture by Toby Gibson. Title: Complexity of PPIs. (IIB-UNSAM)
12:30-1:30 pm: Lunch on own
1:30 - 3:30 pm: Practical Session 4: Understanding linear motifs & intrinsically disordered regions (ELM, SLiMSearch, ProViz).
Coffee Break 3:30-4pm
4:00-6:00 pm: Practical Session 5: Revealing interactive features in protein multiple sequence alignments with Jalview.
Thursday, November 23rd
9:00-10:45 am: Talks:
Gonzalo de Prat Gay. Talk title: Experimental investigation of IDPs.
Ignacio Sánchez. Talk title: Bioinformatics approaches to the study of IDPs.
Coffee break 10:45-11:15 am
11:15-1:15 pm: Practical Session 6: Visualizing protein structures and interaction interfaces with UCSF Chimera.
1:15-2:00 pm: Lunch on own
2:00-3:15 pm: Practical Session 7: Protein association networks with STRING. Protein networks (Reactome/KEGG).
Coffee Break 3:15-3:45 pm
3:45-5:00 pm: Practical Session 8: Practical with you own protein and QA session.
5:00-5:30 pm: Discussion and feedback.
Total duration: 24 hours.
Databases and Software: