FS baskets

Storing high pressure frozen sample prior to freeze substitutionHigh Pressure Freezing is, in 90% of the cases, followed by Freeze Substitution.However, we never considered the FS procedure to be reliable, reproducible an...

Opening the HPF sandwich

This video illustrates how we open the HPF sandwich to select the carrier containing the sample. Then we deposit the sandwich in the FS basket for freeze-substitution processing.

Handling the freeze substitution basket

FS baskets

Opening the HPF sandwich

Exchanging Fluids during FS

Routine HPF manipulation