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Your one stop solution for single objective light-sheet microscopy
Let's make single objective light-sheet microscopy easy and open
Let's make single objective light-sheet microscopy easy and open
Find out what goes behind such a microscope and build yourself one
Preprint doi: 10.1101/2021.04.30.442190
A parts selection GUI tool (Crossbill Design) at zenodo doi: 10.5281/zenodo.3543786
Software and 3D printable files available at github: https://github.com/KozorovitskiyLaboratory/crossbill
Software and 3D printable files available at github: https://github.com/KozorovitskiyLaboratory/crossbill
An interactive parts list 3D blender file at zenodo doi: 10.5281/zenodo.6380689
An optimized design
An optimized design
A new design offering highest performance to cost ratio when compared to other existing designs.
A tailored software
A tailored software
A first of its kind software to control such microscopes. Completely written in python (free to own and modify).
Some random imaging examples
Structural_imaging_Supp_Video2.mp4Structural imaging of thy1GFP mouse brain section (uncleared) and GFP expressing zebrafish larva. These are non-deconvolved 3D data. Only linear contrast adjustments have been applied prior to visualization.
Blood circulation in zebrafish (60x, brightfield mode). No post-processing or contrast mechanism is applied here. Oblique imaging provides a directional shadow providing perceptional depth contrast in the imaging.
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