ESI-HR-MS Lab

Electrospray ionization (ESI) is a frequently used technique for the analysis of polar species of various molecular weights. Direct infusion with ESI coupled with a high-resolution time of flight mass spectrometer (HR-ESI-TOF-MS) allows for a relatively easy and accurate identification, often used in synthetic chemistry. The trainings offered aim to provide practical experience in sample preparation and HR-ESI-TOF-MS analysis, focusing on the main optimization parameters for small molecular weight species and polymers.

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Requirements

Assignment Materials

MassHunter Data Processing

To arrange for a training session and sample analysis using electrospray ionization with high resolution time of flight mass spectrometer (HR TOF MS), each student need prior approval from their advisor. Please contact Dr. Alena Kubatova once you are granted approval to schedule the training.

Requirements

Graduate students are required to accomplish minimum two training sessions while running their own samples under the supervision of TOF operators. Guidelines for the sample preparation and operation of the instrument are available below.

After two sessions, students are expected to proceed with future analysis independently following the approval of the lead instructor.

Instrument will be calibrated by the operators at the beginning of each session.
Prior to each measurement, students need to provide a sheet with chemical structures and exact molecular weights of compounds contained in the sample (if applicable).
All students are expected to backup their own data and share copy in common OneDrive folder.

Before the start of the lab assignment, student must hand-write answers to a take-home test. In addition to providing handwritten answers, students must have knowledge of the content prior to the lab. The lab takes a minimum of 6 hours but it can be broken into several three hour sessions.