Research
Since the earliest days of my time as a retina researcher, I used immunohistochemistry and single-cell dye injections in combination with fluorescence and electron microscopy. Later, I 'went whole-cell' in patch-clamp experiments to record light responses from individual ganglion and amacrine cells before I have put the patch pipettes aside to use 4096 electrodes at once, with CMOS MEAs to record the electrical activity from whole populations of spiking cells. Today, I explore the ultrastructure of retinal circuits in datasets of unprecendented size from multi-beam electron microscopic imaging.
Please visit the publication page for more details of the outcome of my efforts.Â
If you would like to learn more about the retina https://webvision.med.utah.edu/ is a great place to start.
Ganglion cell bodies and axons
Clinched axon bundles
Cone pedicles
Bipolar cell types
Electrical synapses between horizontal cells (Puller et al., 2009)
Components of an electrical synapse (Puller et. al., 2009)
Tight junctions of the OLM
Ganglion and amacrine cells
Ganglion cells (Puller, Duda et al., 2020)
Synapses in the outer plexiform layer