(Project in Independent Study | Aug'17 - May'18)
Cisplatin is an integral part of the current treatment regimen for OS which is the most prevalent primary bone cancer in adolescents. But some recent therapeutic failure of this drug has been linked to microRNAs helping in the development of drug resistance.
To target the miRNAs responsible, this study was performed by doing a comparative study between cells at 4 stages:
Untreated OS cells (G1: OS)
Persister sub-population of cells post drug shock (G2: OS-P)
Cells which evade the negligible growth bottleneck (G3: OS-EP)
Drug-resistant cells obtained after several rounds of drug shock and survival (G4: OS-R)
Furthermore, to better understand the series of genetic events leading to the emergence of drug-resistant cells, a similar study was performed on mRNAs and implications from both these studies can have a future potential therapeutic impact.
(Click here for a detailed report along with the findings!)
(Project in Independent Study | Jan'17 - May'17)
It has been widely known that microRNAs play a key role in the regulation of gene expression but through which mRNA targets and what pathways still remain largely unidentified.
The aim of this study was to unravel some of the key miRNAs, mRNAs, pathways which might be leading to HCC. This was done by deep sequencing miRNAs in HCC cell line and compare it with a control (non-cancerous cell).
The following analyses were done :
Differential analysis
Pathway analysis (w/ Target prediction)
Network analysis
Principal Component Analysis
K-means clustering
Chromosomal Biasedness
(Click here for a detailed report along with the findings!)